FastQCFastQC Report
Wed 25 May 2016
SRR1781158_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781158_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences210761
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCAA200.00694420252.512
TTAAGCA200.00694420252.514
CATCGTT200.00694420252.532
ACGGTTA200.00694420252.545
CGGTATA352.0816227E-550.02
GCCGTTA305.86842E-446.66666862
TCGGTCA305.86842E-446.66666829
CTTCGGA350.001253437440.066
ATTCCAG350.001253437440.033
TTCGGTC350.001253437440.028
TCGTTCC501.7057569E-435.00000462
GCGGTAT501.7057569E-435.0000041
CGGCAAA601.2118468E-535.00000434
TTCCCTA501.7057569E-435.00000426
AGCGTTA400.002414968335.010
CGGAATA400.002414968335.069
GCTTGGG400.002414968335.01
GAGCGTT400.002414968335.09
TTCGGAA400.002414968335.067
GCTTTTA652.0959276E-532.30769327