##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781158_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	210761
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.250060495063128	34.0	32.0	34.0	23.0	34.0
2	31.407143636631066	34.0	33.0	34.0	23.0	34.0
3	31.40977220643288	34.0	33.0	34.0	23.0	34.0
4	31.6143309246018	34.0	34.0	34.0	23.0	34.0
5	31.601833356266102	34.0	34.0	34.0	23.0	34.0
6	34.38062544778209	37.0	34.0	38.0	25.0	38.0
7	34.265495039404826	37.0	34.0	38.0	24.0	38.0
8	33.99329572359213	37.0	34.0	38.0	24.0	38.0
9	34.37030570171901	37.0	34.0	38.0	25.0	38.0
10-11	34.25838271786526	37.0	34.0	38.0	24.0	38.0
12-13	34.21740027804006	37.0	34.0	38.0	24.0	38.0
14-15	34.11727739002947	37.0	34.0	38.0	24.0	38.0
16-17	34.110836445072856	37.0	34.0	38.0	23.5	38.0
18-19	34.14201156760501	37.0	34.0	38.0	24.0	38.0
20-21	34.06958830144097	37.0	34.0	38.0	23.5	38.0
22-23	34.143800323589275	37.0	34.0	38.0	24.0	38.0
24-25	34.12245386954892	37.0	34.0	38.0	24.0	38.0
26-27	34.06030764705045	37.0	34.0	38.0	23.5	38.0
28-29	34.137731838433105	37.0	34.0	38.0	24.0	38.0
30-31	34.20809115538454	37.0	34.0	38.0	24.0	38.0
32-33	34.165163384117555	37.0	34.0	38.0	24.0	38.0
34-35	34.18974335859101	37.0	34.0	38.0	24.0	38.0
36-37	34.03008621139585	37.0	34.0	38.0	23.5	38.0
38-39	34.04960832412068	37.0	34.0	38.0	24.0	38.0
40-41	34.047150563908886	37.0	34.0	38.0	24.0	38.0
42-43	34.11441158468597	37.0	34.0	38.0	24.0	38.0
44-45	34.161277465944835	37.0	34.0	38.0	24.0	38.0
46-47	34.14524271568269	37.0	34.0	38.0	24.0	38.0
48-49	34.111023861150784	37.0	34.0	38.0	24.0	38.0
50-51	33.99467406208929	37.0	34.0	38.0	23.5	38.0
52-53	34.07857715611522	37.0	34.0	38.0	24.0	38.0
54-55	34.049703218337356	37.0	34.0	38.0	24.0	38.0
56-57	33.998567097328255	37.0	34.0	38.0	23.5	38.0
58-59	34.01013707469598	37.0	34.0	38.0	24.0	38.0
60-61	33.97840207628546	37.0	34.0	38.0	23.5	38.0
62-63	33.95217094244191	37.0	34.0	38.0	23.5	38.0
64-65	33.973386916934345	37.0	34.0	38.0	23.5	38.0
66-67	33.84477441272342	37.0	34.0	38.0	23.0	38.0
68-69	33.61520395139518	37.0	34.0	38.0	22.5	38.0
70-71	33.79898795317919	37.0	34.0	38.0	23.0	38.0
72-73	33.78344902519916	37.0	34.0	38.0	23.0	38.0
74-75	33.76741664729243	37.0	34.0	38.0	23.0	38.0
76	32.38059697951709	37.0	31.0	38.0	21.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	26.0
14	93.0
15	295.0
16	659.0
17	1111.0
18	1518.0
19	1718.0
20	1738.0
21	1669.0
22	1740.0
23	2022.0
24	2326.0
25	2846.0
26	3467.0
27	4066.0
28	4779.0
29	5635.0
30	6764.0
31	8451.0
32	10188.0
33	12454.0
34	15858.0
35	21258.0
36	32531.0
37	67546.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.22136448394153	18.39382048861032	10.110029844231143	26.27478518321701
2	22.71909888451848	19.574779015092926	32.599484724403474	25.10663737598512
3	20.117099463373204	21.83895502488601	28.44027120767125	29.60367430406954
4	14.267819947713287	15.72397170254459	37.091776941654295	32.91643140808783
5	13.976494702530355	34.12490925740531	35.68639359274249	16.212202447321847
6	30.992925636147106	36.099657906348895	17.979132761753835	14.928283695750164
7	30.421662451781877	28.16128221065567	22.086628930399836	19.330426407162616
8	26.504903658646523	33.44641560820076	19.88982781444385	20.15885291870887
9	24.883161495722643	15.985879740559211	21.719862782962693	37.411095980755455
10-11	25.807668401649263	24.206091259768172	27.228472060770258	22.75776827781231
12-13	25.48194400292274	22.654570817181547	26.801210850204733	25.062274329690975
14-15	23.59615868210912	23.882501980916775	23.847153885206467	28.67418545176764
16-17	24.05592116188479	26.530999568231312	25.23972651486755	24.173352755016346
18-19	23.636014253111345	26.03494005057862	26.389607185390084	23.939438510919953
20-21	23.388814818680874	26.46552255872766	26.32982382888675	23.815838793704717
22-23	23.734467002908506	25.67837503143371	26.82113863570585	23.766019329951938
24-25	23.34682412780353	26.19151550808736	26.46172679006078	23.99993357404833
26-27	22.634643031680433	26.48568757977045	26.677848368531183	24.201821020017935
28-29	23.48157391547772	25.90066473398779	27.443881932615614	23.173879417918876
30-31	23.474694084769006	26.061273195705088	26.86834850850015	23.59568421102576
32-33	23.08942356508083	26.38059223480625	26.68259307936478	23.847391120748146
34-35	23.92022243204388	25.490958953506578	26.81473327608049	23.77408533836905
36-37	23.845967707498065	25.554063607593434	26.399333842598967	24.200634842309533
38-39	23.13093978487481	26.253671220007497	27.14045767480701	23.47493132031068
40-41	23.507195353979153	25.12585345486119	27.644820436418506	23.72213075474115
42-43	23.124534425249454	25.290257685245372	27.388606051404196	24.196601838100975
44-45	22.37154881595741	24.932269252850386	27.985016203187495	24.71116572800471
46-47	22.67188901172418	24.663718619668725	28.152267260071834	24.51212510853526
48-49	21.954488733684123	24.558860510246202	28.972390527659293	24.51426022841038
50-51	21.910600158473343	24.75434259659045	28.542282490593614	24.7927747543426
52-53	21.381090429443777	24.659448379918487	29.266562599342382	24.692898591295354
54-55	20.824298613121023	25.252774469659943	29.078672050331893	24.844254866887137
56-57	20.58990989794127	25.42666812171132	28.91806358861459	25.06535839173282
58-59	21.126536693221233	25.2769724949113	28.261158373702916	25.335332438164553
60-61	20.822875199870943	25.021707052063714	28.387842152959987	25.767575595105356
62-63	20.45800693676724	24.695270946712153	28.88271549290429	25.964006623616324
64-65	21.236613984560712	24.819819606094107	27.991421562812857	25.952144846532327
66-67	21.274808906771177	24.410825532237936	27.67400040804513	26.640365152945755
68-69	21.148836834139143	24.70594654608775	27.305573611816225	26.83964300795688
70-71	21.781069552716108	24.86774118551345	27.103211694763264	26.24797756700718
72-73	21.751889581089483	24.611052329415784	26.68022072394798	26.956837365546754
74-75	21.59602583020578	25.12846304581967	26.584140329567614	26.691370794406932
76	22.32481341424647	24.869401834305208	25.943604367031853	26.862180384416472
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.5
2	0.5
3	0.0
4	0.0
5	0.0
6	0.0
7	1.0
8	2.0
9	1.0
10	1.0
11	2.5
12	3.0
13	4.0
14	7.5
15	10.0
16	20.0
17	30.0
18	48.5
19	76.5
20	128.5
21	171.0
22	210.0
23	316.0
24	504.0
25	625.0
26	739.0
27	934.5
28	1228.5
29	1441.0
30	1681.0
31	2182.0
32	2931.0
33	3419.0
34	3909.5
35	4978.0
36	6125.5
37	6695.0
38	7297.5
39	8767.5
40	10496.0
41	12023.5
42	12690.0
43	12926.5
44	13406.5
45	13566.5
46	13483.0
47	13323.0
48	12357.5
49	11021.0
50	10490.0
51	9620.0
52	7915.5
53	6486.0
54	5891.0
55	5386.5
56	4454.0
57	3634.0
58	3242.0
59	2950.0
60	2596.5
61	2342.5
62	2150.0
63	2118.0
64	1991.0
65	1908.0
66	1908.0
67	1896.0
68	1904.5
69	1885.0
70	1834.5
71	1812.0
72	1748.5
73	1657.0
74	1532.0
75	1435.0
76	1320.0
77	1102.0
78	912.5
79	826.0
80	724.0
81	533.5
82	375.5
83	306.0
84	253.0
85	173.5
86	113.5
87	80.0
88	61.5
89	40.0
90	24.5
91	12.0
92	12.0
93	7.5
94	2.0
95	3.0
96	5.0
97	2.5
98	0.0
99	1.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	210761.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.837764478459334
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.84341423210662	34.598339791175576
2	14.298048160393629	13.965694171179454
3	5.567549290454907	8.157199829083503
4	2.4726804570113474	4.830407431600375
5	1.346682073556878	3.2884470967867028
6	0.8811347660958787	2.581959130822373
7	0.7372758302724608	2.52048323481549
8	0.5644455758329849	2.205300807475174
9	0.41459276734853456	1.8223005533616374
>10	2.8232268923931945	24.451516604518982
>50	0.04895191710085915	1.4641831217593093
>100	0.0019980374326881286	0.114168227421419
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	9.489421667196493E-4	0.0
2	0.0	0.0	0.0	9.489421667196493E-4	0.0
3	0.0	0.0	0.0	9.489421667196493E-4	0.0
4	0.0	0.0	0.0	0.001423413250079474	0.0
5	0.0	0.0	0.0	0.001423413250079474	0.0
6	0.0	0.0	0.0	0.001423413250079474	0.0
7	0.0	0.0	0.0	0.001423413250079474	0.0
8	0.0	0.0	0.0	0.001423413250079474	0.0
9	0.0	0.0	0.0	0.001423413250079474	0.0
10	0.0	0.0	0.0	0.0018978843334392986	0.0
11	0.0	0.0	0.0	0.002372355416799123	0.0
12	0.0	0.0	0.0	0.002372355416799123	0.0
13	0.0	4.7447108335982465E-4	0.0	0.002846826500158948	0.0
14	0.0	4.7447108335982465E-4	0.0	0.002846826500158948	0.0
15	0.0	4.7447108335982465E-4	0.0	0.002846826500158948	0.0
16	0.0	4.7447108335982465E-4	0.0	0.002846826500158948	0.0
17	0.0	4.7447108335982465E-4	0.0	0.002846826500158948	0.0
18	0.0	4.7447108335982465E-4	0.0	0.0033212975835187723	0.0
19	0.0	4.7447108335982465E-4	0.0	0.003795768666878597	0.0018978843334392986
20	0.0	4.7447108335982465E-4	0.0	0.004270239750238422	0.0018978843334392986
21	0.0	4.7447108335982465E-4	0.0	0.004270239750238422	0.0018978843334392986
22	0.0	4.7447108335982465E-4	0.0	0.004270239750238422	0.0018978843334392986
23	0.0	4.7447108335982465E-4	0.0	0.005219181916958071	0.0018978843334392986
24	0.0	4.7447108335982465E-4	0.0	0.005693653000317896	0.0018978843334392986
25	0.0	4.7447108335982465E-4	0.0	0.007591537333757194	0.0018978843334392986
26	0.0	4.7447108335982465E-4	0.0	0.009014950583836667	0.0018978843334392986
27	0.0	4.7447108335982465E-4	0.0	0.018029901167673335	0.0018978843334392986
28	0.0	4.7447108335982465E-4	0.0	0.03511086016862702	0.0018978843334392986
29	0.0	4.7447108335982465E-4	0.0	0.05598758783645931	0.0018978843334392986
30	0.0	4.7447108335982465E-4	0.0	0.07496643117085229	0.0018978843334392986
31	0.0	9.489421667196493E-4	0.0	0.09489421667196493	0.0018978843334392986
32	0.0	9.489421667196493E-4	0.0	0.11387306000635791	0.0018978843334392986
33	0.0	9.489421667196493E-4	0.0	0.15277968884186352	0.0018978843334392986
34	0.0	9.489421667196493E-4	0.0	0.2097162188450425	0.0018978843334392986
35	0.0	9.489421667196493E-4	0.0	0.2875294765160537	0.0018978843334392986
36	0.0	9.489421667196493E-4	0.0	0.43176868585744044	0.0018978843334392986
37	0.0	9.489421667196493E-4	0.0	0.6690042275373528	0.0018978843334392986
38	0.0	9.489421667196493E-4	0.0	0.9940169196388327	0.0018978843334392986
39	0.0	9.489421667196493E-4	0.0	1.4115514729954783	0.0018978843334392986
40	0.0	9.489421667196493E-4	0.0	1.8988332756060182	0.0018978843334392986
41	0.0	9.489421667196493E-4	0.0	2.4492197323034146	0.0018978843334392986
42	0.0	9.489421667196493E-4	0.0	3.0427830575865555	0.0018978843334392986
43	0.0	9.489421667196493E-4	0.0	3.7563875669597317	0.0018978843334392986
44	0.0	9.489421667196493E-4	0.0	4.548754276170639	0.0018978843334392986
45	0.0	9.489421667196493E-4	0.0	5.4848857236395725	0.0018978843334392986
46	0.0	9.489421667196493E-4	0.0	6.4067830386077125	0.0018978843334392986
47	0.0	9.489421667196493E-4	0.0	7.30543127049122	0.0018978843334392986
48	0.0	9.489421667196493E-4	0.0	8.251052139627351	0.0018978843334392986
49	0.0	9.489421667196493E-4	0.0	9.161087677511494	0.0018978843334392986
50	0.0	9.489421667196493E-4	0.0	9.979550296307192	0.0018978843334392986
51	0.0	9.489421667196493E-4	0.0	10.798961857269608	0.0018978843334392986
52	0.0	9.489421667196493E-4	0.0	11.626913897732504	0.0018978843334392986
53	0.0	9.489421667196493E-4	0.0	12.439208392444522	0.0018978843334392986
54	0.0	9.489421667196493E-4	0.0	13.233472985988868	0.0018978843334392986
55	0.0	9.489421667196493E-4	0.0	14.166283135874284	0.0018978843334392986
56	0.0	9.489421667196493E-4	0.0	15.055916417173956	0.0018978843334392986
57	0.0	9.489421667196493E-4	0.0	15.872955622719573	0.0018978843334392986
58	0.0	9.489421667196493E-4	0.0	16.70043319209911	0.0018978843334392986
59	0.0	9.489421667196493E-4	0.0	17.51130427356105	0.0018978843334392986
60	0.0	9.489421667196493E-4	0.0	18.368199050108892	0.0018978843334392986
61	0.0	9.489421667196493E-4	0.0	19.198048974905223	0.0018978843334392986
62	0.0	9.489421667196493E-4	0.0	19.986145444365892	0.0018978843334392986
63	0.0	9.489421667196493E-4	0.0	20.737707640407855	0.0018978843334392986
64	0.0	9.489421667196493E-4	0.0	21.50635079545077	0.0018978843334392986
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACCAA	20	0.006944202	52.5	12
TTAAGCA	20	0.006944202	52.5	14
CATCGTT	20	0.006944202	52.5	32
ACGGTTA	20	0.006944202	52.5	45
CGGTATA	35	2.0816227E-5	50.0	2
GCCGTTA	30	5.86842E-4	46.666668	62
TCGGTCA	30	5.86842E-4	46.666668	29
CTTCGGA	35	0.0012534374	40.0	66
ATTCCAG	35	0.0012534374	40.0	33
TTCGGTC	35	0.0012534374	40.0	28
TCGTTCC	50	1.7057569E-4	35.000004	62
GCGGTAT	50	1.7057569E-4	35.000004	1
CGGCAAA	60	1.2118468E-5	35.000004	34
TTCCCTA	50	1.7057569E-4	35.000004	26
AGCGTTA	40	0.0024149683	35.0	10
CGGAATA	40	0.0024149683	35.0	69
GCTTGGG	40	0.0024149683	35.0	1
GAGCGTT	40	0.0024149683	35.0	9
TTCGGAA	40	0.0024149683	35.0	67
GCTTTTA	65	2.0959276E-5	32.307693	27
>>END_MODULE