FastQCFastQC Report
Wed 25 May 2016
SRR1781158_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781158_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences191355
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT34901.8238352799770061No Hit
TCTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCG3500.18290611690313816No Hit
CTTATACACATCTCCGAGCCCACGAGACAAATACAGACCTCGTATGCCGT2840.14841524914426069No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCC2510.1311698152648219No Hit
CTTATACACATCTCCGAGCCCACGAGACAAATACAGAACTCGTATGCCGT2120.11078884795275795No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG5200.056.5384640
CGTATGC5250.056.041
CTCGTAT5050.055.44554539
TATGCCG5250.055.33333243
CCGTCTT5050.054.75247647
GTATGCC5400.054.44444742
GCCGTCT5250.053.33333246
CGTCTTC5150.053.00971248
ATGCCGT5350.052.99065444
TCTTCTG5000.052.50000450
TAGTTTC200.006943229652.535
AGCGCTG200.006943229652.561
TCTCGTA4150.052.28915438
ATCTCGT4200.051.66666837
CTTGAAA5450.051.3761557
TGCCGTC5500.050.9090945
ACAGATC4250.050.23529433
AGATCTC4350.049.08046335
CAGATCT4350.049.08046334
ACTCGTA506.165101E-848.99999638