##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781158_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	191355
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.193880483917326	34.0	34.0	34.0	32.0	34.0
2	33.239314363356065	34.0	34.0	34.0	32.0	34.0
3	33.30190483656032	34.0	34.0	34.0	33.0	34.0
4	33.369162028690134	34.0	34.0	34.0	33.0	34.0
5	33.353196937629015	34.0	34.0	34.0	33.0	34.0
6	36.7623161140289	38.0	37.0	38.0	34.0	38.0
7	36.65449557105902	38.0	37.0	38.0	34.0	38.0
8	36.44042747772465	38.0	37.0	38.0	34.0	38.0
9	36.37314938203862	38.0	37.0	38.0	34.0	38.0
10-11	36.29828852133469	38.0	37.0	38.0	34.0	38.0
12-13	36.23525384756081	38.0	37.0	38.0	34.0	38.0
14-15	36.24756604217292	38.0	37.0	38.0	34.0	38.0
16-17	36.191763998850305	38.0	37.0	38.0	33.5	38.0
18-19	36.177685453737816	38.0	37.0	38.0	33.5	38.0
20-21	36.12156463118288	38.0	37.0	38.0	32.5	38.0
22-23	36.18326670324789	38.0	37.0	38.0	33.5	38.0
24-25	36.18390426171253	38.0	37.0	38.0	33.5	38.0
26-27	35.96012907945964	38.0	36.5	38.0	31.5	38.0
28-29	35.934898487105116	38.0	36.0	38.0	32.0	38.0
30-31	35.97266076141204	38.0	37.0	38.0	32.0	38.0
32-33	35.92706487941261	38.0	36.5	38.0	32.0	38.0
34-35	35.915264821927835	38.0	36.5	38.0	32.0	38.0
36-37	35.85890622142092	38.0	36.0	38.0	31.0	38.0
38-39	35.675892973792166	38.0	36.0	38.0	31.0	38.0
40-41	35.77873847038227	38.0	36.0	38.0	31.0	38.0
42-43	35.84325207075854	38.0	36.0	38.0	31.0	38.0
44-45	35.81410206161323	38.0	36.0	38.0	31.0	38.0
46-47	35.83282903503958	38.0	36.0	38.0	31.0	38.0
48-49	35.821619503017956	38.0	36.0	38.0	31.0	38.0
50-51	35.71687178281205	38.0	36.0	38.0	31.0	38.0
52-53	35.74361788299234	38.0	36.0	38.0	31.0	38.0
54-55	35.75476470434533	38.0	36.0	38.0	31.0	38.0
56-57	35.70481043087455	38.0	36.0	38.0	31.0	38.0
58-59	35.689030858874865	38.0	36.0	38.0	31.0	38.0
60-61	35.6908024352643	38.0	36.0	38.0	31.0	38.0
62-63	35.670100075775395	38.0	36.0	38.0	31.0	38.0
64-65	35.61582399205665	38.0	36.0	38.0	31.0	38.0
66-67	35.5641033680855	38.0	36.0	38.0	31.0	38.0
68-69	35.46531838729064	38.0	36.0	38.0	31.0	38.0
70-71	35.31200125421337	38.0	36.0	38.0	29.0	38.0
72-73	35.16850095372475	38.0	36.0	38.0	27.0	38.0
74-75	35.20123069687231	38.0	36.0	38.0	27.0	38.0
76	34.2587024117478	37.0	34.0	38.0	25.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	4.0
21	7.0
22	24.0
23	72.0
24	147.0
25	297.0
26	601.0
27	1044.0
28	1776.0
29	2951.0
30	4380.0
31	6356.0
32	9078.0
33	12213.0
34	16473.0
35	22217.0
36	32626.0
37	81088.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.17501502443103	19.292937210943013	10.278278592145488	28.25376917248047
2	22.198008936270284	19.34415092367589	33.154085338768255	25.303754801285567
3	19.665543100519976	22.144704867915653	29.224739358783413	28.965012672780954
4	14.033079877714197	17.798332941391653	36.02362101852577	32.14496616236837
5	13.818818426484805	33.91131666274725	36.75158736380026	15.51827754696768
6	30.323221238013115	38.63395260118628	16.63818557132032	14.404640589480286
7	29.303127694599045	28.357764364662536	21.12722426902877	21.21188367170965
8	25.503383763162706	35.53029709179274	18.516370097462833	20.44994904758172
9	24.273209479762745	15.630634161636747	19.926314964333308	40.169841394267195
10-11	25.232944004598785	25.115361500875338	27.117138303153826	22.534556191372058
12-13	24.69702908207259	22.352172663374358	26.908102740978805	26.042695513574248
14-15	22.700739463301193	23.4762613989705	22.92649787045021	30.896501267278097
16-17	24.46029630790938	27.362755088709466	24.1681691097698	24.00877949361135
18-19	24.47179326382901	25.85168926863683	25.003527475111703	24.672989992422462
20-21	22.58733767082125	26.026756551958403	25.039324815134172	26.346580962086176
22-23	23.2005957513522	26.823704632750644	25.41611141595464	24.559588199942514
24-25	24.19978573854877	26.767265030963394	25.27553500039194	23.757414230095893
26-27	23.406495780094588	27.292205586475397	25.63089545608947	23.670403177340546
28-29	23.037809307308404	26.86525045073293	26.12291291055891	23.974027331399757
30-31	22.874761568811895	28.255859528102217	25.610253194324685	23.259125708761204
32-33	22.187557158161532	27.727783439157587	26.80593660996577	23.27872279271511
34-35	23.156959577748164	26.53471296804369	25.904993336991456	24.40333411721669
36-37	24.404379295027567	26.69802200099292	25.143320007316245	23.75427869666327
38-39	22.454338794387397	26.213059496746883	26.985184604530847	24.347417104334877
40-41	22.60641216586972	24.821143947114003	27.749732173185965	24.822711713830316
42-43	23.644796320974105	25.054218598939144	27.450027435917534	23.850957644169217
44-45	21.734472577147187	26.085286509367407	28.075305061273546	24.104935852211856
46-47	23.318700843981084	24.38922421676988	27.109038175119544	25.183036764129497
48-49	22.335188523947636	24.48224504193776	27.571006767526324	25.611559666588278
50-51	21.40079956102532	24.42528285124507	28.641007551409686	25.53291003631993
52-53	20.708369261320584	24.217292466880927	30.4844399153406	24.589898356457894
54-55	19.848971806328553	24.905542055342163	29.269943299103762	25.975542839225525
56-57	20.921846829191818	24.86399623735988	27.910689556060724	26.30346737738758
58-59	19.96603172114656	24.648167019414178	29.604400198583786	25.78140106085548
60-61	20.833006715267434	25.888531786470175	27.448198374748507	25.830263123513888
62-63	19.290585560868543	27.07559248517154	27.348645188262655	26.285176765697265
64-65	19.680175589872224	27.534425544145698	26.144339055681847	26.641059810300227
66-67	19.644900838755195	27.22583679548483	25.764939510334194	27.364322855425776
68-69	19.737399075017635	27.658017820281678	25.364375114316324	27.240207990384363
70-71	19.956886415301405	27.44062083561966	25.0424603485668	27.560032400512135
72-73	20.06114290193619	26.543596979436128	24.979488385461575	28.415771733166107
74-75	19.66502051161454	27.237856340309897	24.839173264351597	28.25794988372397
76	20.395077212510778	27.53573201640929	24.190117843798177	27.879072927281754
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	1.0
14	5.0
15	15.0
16	30.5
17	39.0
18	53.5
19	79.5
20	109.0
21	127.0
22	177.0
23	283.0
24	443.5
25	548.0
26	639.5
27	818.0
28	1117.0
29	1329.0
30	1536.0
31	1887.5
32	2505.5
33	2979.0
34	3338.0
35	4229.0
36	5297.0
37	5833.0
38	6678.0
39	8624.0
40	10610.0
41	11849.0
42	12203.0
43	12221.5
44	12188.0
45	11999.5
46	11863.0
47	11690.5
48	10837.5
49	9713.5
50	9270.0
51	8649.5
52	7020.5
53	5632.5
54	5253.0
55	4865.0
56	4026.5
57	3206.5
58	2837.0
59	2602.5
60	2194.5
61	1873.0
62	1725.0
63	1748.0
64	1735.5
65	1722.0
66	1751.5
67	1759.0
68	1773.0
69	1787.5
70	1715.0
71	1642.0
72	1609.0
73	1580.0
74	1445.5
75	1307.0
76	1263.5
77	1114.5
78	882.0
79	755.0
80	678.5
81	518.0
82	374.0
83	314.0
84	262.0
85	160.0
86	86.0
87	62.0
88	48.5
89	27.5
90	14.5
91	7.0
92	5.0
93	4.0
94	1.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	191355.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.14237934728646
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.21329133218168	25.18460453084581
2	19.153287267554113	15.760236210185258
3	7.614825729092573	9.398761464294113
4	3.2961589269383196	5.42447283844164
5	1.775734173356366	3.6528964490083875
6	1.1584188598719642	2.8596064905542056
7	0.8815161060867799	2.5387369026155575
8	0.6630423737425059	2.182331269107157
9	0.5372929580327203	1.9894959630007054
>10	3.6048165836805204	26.030675968749183
>50	0.09018392439792704	2.3380627629275432
>100	0.0101615689462453	0.8162838702934336
>500	0.0	0.0
>1k	0.0012701961182806625	1.8238352799770061
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT	3490	1.8238352799770061	No Hit
TCTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCG	350	0.18290611690313816	No Hit
CTTATACACATCTCCGAGCCCACGAGACAAATACAGACCTCGTATGCCGT	284	0.14841524914426069	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCC	251	0.1311698152648219	No Hit
CTTATACACATCTCCGAGCCCACGAGACAAATACAGAACTCGTATGCCGT	212	0.11078884795275795	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	5.225889054375376E-4	0.0
2	0.0	0.0	0.0	5.225889054375376E-4	0.0
3	0.0	0.0	0.0	5.225889054375376E-4	0.0
4	0.0	0.0	0.0	0.0010451778108750752	0.0
5	0.0	0.0	0.0	0.0010451778108750752	0.0
6	0.0	0.0	0.0	0.0010451778108750752	0.0
7	0.0	0.0	0.0	0.0010451778108750752	0.0
8	0.0	0.0	0.0	0.0010451778108750752	0.0
9	0.0	0.0	0.0	0.0015677667163126127	0.0
10	0.0	0.0	0.0	0.0020903556217501504	0.0
11	0.0	0.0	0.0	0.002612944527187688	0.0
12	0.0	0.0	0.0	0.002612944527187688	0.0
13	0.0	0.0	0.0	0.0031355334326252254	0.0
14	0.0	0.0	0.0	0.0031355334326252254	0.0
15	0.0	0.0	0.0	0.0031355334326252254	0.0
16	0.0	0.0	0.0	0.0031355334326252254	0.0
17	0.0	0.0	0.0	0.0031355334326252254	0.0
18	0.0	0.0	0.0	0.003658122338062763	0.0
19	0.0	0.0	0.0	0.004180711243500301	0.0
20	0.0	0.0	0.0	0.004703300148937838	0.0
21	0.0	0.0	0.0	0.004703300148937838	0.0
22	0.0	0.0	0.0	0.004703300148937838	0.0
23	0.0	0.0	0.0	0.007838833581563063	0.0
24	0.0	0.0	0.0	0.008361422487000602	0.0
25	0.0	0.0	0.0	0.010451778108750752	0.0
26	0.0	0.0	0.0	0.012019544825063363	0.0
27	0.0	0.0	0.0	0.022471322933814115	0.0
28	0.0	0.0	0.0	0.041284523529565464	0.0
29	0.0	0.0	0.0	0.0653236131796922	0.0
30	0.0	0.0	0.0	0.08570458049175617	0.0
31	0.0	0.0	0.0	0.10869849233100781	0.0
32	0.0	0.0	0.0	0.12960204854850932	0.0
33	0.0	0.0	0.0	0.17036398317263723	0.0
34	0.0	0.0	0.0	0.23464241854145437	0.0
35	0.0	0.0	0.0	0.31616628778971023	0.0
36	0.0	0.0	0.0	0.47816884847534685	0.0
37	0.0	0.0	0.0	0.7384181233832405	0.0
38	0.0	0.0	0.0	1.091688223459016	0.0
39	0.0	0.0	0.0	1.537979148702673	0.0
40	0.0	0.0	0.0	2.044367798071647	0.0
41	0.0	0.0	0.0	2.6124219382822504	0.0
42	0.0	0.0	0.0	3.2416189804290454	0.0
43	0.0	0.0	0.0	3.9972825376917247	0.0
44	0.0	0.0	0.0	4.83446996420266	0.0
45	0.0	0.0	0.0	5.822685584385043	0.0
46	0.0	0.0	0.0	6.78477175929555	0.0
47	0.0	0.0	0.0	7.7317028559483685	0.0
48	0.0	0.0	0.0	8.7052859867785	0.0
49	0.0	0.0	0.0	9.653262261242194	0.0
50	0.0	0.0	0.0	10.510308066159755	0.0
51	0.0	0.0	0.0	11.348018081576129	0.0
52	0.0	0.0	0.0	12.213947897886127	0.0
53	0.0	0.0	0.0	13.060541924694938	0.0
54	0.0	0.0	0.0	13.89198087324606	0.0
55	0.0	0.0	0.0	14.86660918188707	0.0
56	0.0	0.0	0.0	15.791068955606072	0.0
57	0.0	0.0	0.0	16.669540905646574	0.0
58	0.0	0.0	0.0	17.51456716573907	0.0
59	0.0	0.0	0.0	18.38258733767082	0.0
60	0.0	0.0	0.0	19.26785294348201	0.0
61	0.0	0.0	0.0	20.13848605994095	0.0
62	0.0	0.0	0.0	20.962086174910507	0.0
63	0.0	0.0	0.0	21.73185963262	0.0
64	0.0	0.0	0.0	22.527239946695932	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTATG	520	0.0	56.53846	40
CGTATGC	525	0.0	56.0	41
CTCGTAT	505	0.0	55.445545	39
TATGCCG	525	0.0	55.333332	43
CCGTCTT	505	0.0	54.752476	47
GTATGCC	540	0.0	54.444447	42
GCCGTCT	525	0.0	53.333332	46
CGTCTTC	515	0.0	53.009712	48
ATGCCGT	535	0.0	52.990654	44
TCTTCTG	500	0.0	52.500004	50
TAGTTTC	20	0.0069432296	52.5	35
AGCGCTG	20	0.0069432296	52.5	61
TCTCGTA	415	0.0	52.289154	38
ATCTCGT	420	0.0	51.666668	37
CTTGAAA	545	0.0	51.37615	57
TGCCGTC	550	0.0	50.90909	45
ACAGATC	425	0.0	50.235294	33
AGATCTC	435	0.0	49.080463	35
CAGATCT	435	0.0	49.080463	34
ACTCGTA	50	6.165101E-8	48.999996	38
>>END_MODULE