FastQCFastQC Report
Wed 25 May 2016
SRR1781157_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781157_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences284657
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTGT509.786163E-1055.99999634
ACGGTAA200.006946691352.55
CAATACG506.179471E-848.99999669
GGTCTAA305.87191E-446.6666681
CATCGTT659.978976E-943.07692332
GAACACC753.526111E-837.33333254
CCACGAC951.8735591E-1036.8421029
AACCACG904.483809E-935.0000047
TGCTTCG501.7072588E-435.052
GCTCTAC501.7072588E-435.01
TTGCGAT501.7072588E-435.016
CATGCAA652.0984982E-532.30769337
CTTCGCA552.9878403E-431.8181854
CATCGCA552.9878403E-431.8181826
CGACCAA1001.2532837E-831.49999812
CTTCGTC450.0043030731.11111327
GGTATTG450.0043030731.1111131
TTCGGGC450.0043030731.11111346
AACTACA901.7508319E-731.11111346
GCTAAAC450.0043030731.11111356