FastQCFastQC Report
Wed 25 May 2016
SRR1781157_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781157_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences257809
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGT27841.0798692054970926No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCG2930.11365002773370983No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCG150.002222941770.04
AACTCGT150.002222941770.037
GCATATA150.002222941770.01
CGTATGC4250.058.4705941
TATGCCG4200.058.33333243
TCGTATG4250.057.64705740
ATGCCGT4150.057.349444
CTCGTAT4100.057.1951239
CCGTCTT4050.057.03703747
GCCGTCT4200.056.66666446
TCTCGTA3600.056.38888538
CCAACGT252.3875473E-456.00000422
ATCTCGT3650.055.61644437
CGTCTTC4050.055.3086448
TGCCGTC4350.054.71264645
CATCTCG3750.054.13333536
CAACTCG200.00694595352.536
GTATGCC4700.052.1276642
CTTGAAA4400.050.90909657
GCATCTC4000.050.75000435
Produced by FastQC (version 0.11.5)