##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781157_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	257809
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.21868902947531	34.0	34.0	34.0	32.0	34.0
2	33.235127555671056	34.0	34.0	34.0	32.0	34.0
3	33.32155588051619	34.0	34.0	34.0	33.0	34.0
4	33.39223611278117	34.0	34.0	34.0	33.0	34.0
5	33.3745059326866	34.0	34.0	34.0	33.0	34.0
6	36.79546873848469	38.0	37.0	38.0	34.0	38.0
7	36.70309802993689	38.0	37.0	38.0	34.0	38.0
8	36.469611223812976	38.0	37.0	38.0	34.0	38.0
9	36.418488105535495	38.0	37.0	38.0	34.0	38.0
10-11	36.330952371717046	38.0	37.0	38.0	34.0	38.0
12-13	36.27779286215764	38.0	37.0	38.0	34.0	38.0
14-15	36.27392565814227	38.0	37.0	38.0	34.0	38.0
16-17	36.21931352280177	38.0	37.0	38.0	34.0	38.0
18-19	36.22894080501456	38.0	37.0	38.0	34.0	38.0
20-21	36.16812640365542	38.0	37.0	38.0	33.0	38.0
22-23	36.23016651862425	38.0	37.0	38.0	34.0	38.0
24-25	36.22570973084726	38.0	37.0	38.0	34.0	38.0
26-27	35.9979442145154	38.0	37.0	38.0	32.0	38.0
28-29	35.99841743306091	38.0	37.0	38.0	32.0	38.0
30-31	36.02474506320571	38.0	37.0	38.0	32.0	38.0
32-33	35.96513504183329	38.0	37.0	38.0	32.0	38.0
34-35	35.97814661241462	38.0	37.0	38.0	32.0	38.0
36-37	35.933516673196046	38.0	37.0	38.0	32.0	38.0
38-39	35.872376449231794	38.0	37.0	38.0	32.0	38.0
40-41	35.91529388035328	38.0	37.0	38.0	32.0	38.0
42-43	35.933625280731086	38.0	37.0	38.0	32.0	38.0
44-45	35.90837014999477	38.0	36.5	38.0	32.0	38.0
46-47	35.93682338475383	38.0	37.0	38.0	32.0	38.0
48-49	35.90478610133859	38.0	37.0	38.0	32.0	38.0
50-51	35.81166095830635	38.0	36.0	38.0	31.0	38.0
52-53	35.841818943481414	38.0	36.0	38.0	31.0	38.0
54-55	35.846064334449146	38.0	36.0	38.0	31.0	38.0
56-57	35.814106567264915	38.0	36.0	38.0	31.0	38.0
58-59	35.80534426649186	38.0	36.0	38.0	31.0	38.0
60-61	35.79279815677498	38.0	36.0	38.0	31.0	38.0
62-63	35.776978305644874	38.0	36.0	38.0	31.0	38.0
64-65	35.73838966056266	38.0	36.0	38.0	31.0	38.0
66-67	35.70646292410272	38.0	36.0	38.0	31.0	38.0
68-69	35.639925293531256	38.0	36.0	38.0	31.0	38.0
70-71	35.55487783591729	38.0	36.0	38.0	31.0	38.0
72-73	35.49738178263753	38.0	36.0	38.0	31.0	38.0
74-75	35.493085966742825	38.0	36.0	38.0	31.0	38.0
76	34.60214344728074	37.0	34.0	38.0	27.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	5.0
21	9.0
22	40.0
23	95.0
24	200.0
25	366.0
26	710.0
27	1316.0
28	2171.0
29	3477.0
30	5362.0
31	7912.0
32	11315.0
33	15931.0
34	21393.0
35	29816.0
36	44789.0
37	112902.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.616681341613365	17.817066122594635	9.176948826456796	26.389303709335206
2	24.401785818183228	20.49889646986723	30.808466733124135	24.29085097882541
3	20.23009282065405	22.17377981373808	29.7107548611569	27.885372504450967
4	13.88004297755315	17.708070703505307	37.43740521083438	30.974481108107167
5	13.289683447823778	33.92744240891513	37.11546144626448	15.667412696996614
6	29.27748837317549	38.98234739671617	18.304248494040163	13.435915736068175
7	28.869434348684493	30.0039952057531	21.463951995469515	19.662618450092896
8	25.426575488055107	35.78657067829285	18.927578168333923	19.859275665318123
9	24.84125845102382	16.023490258291993	20.166867719901166	38.96838357078302
10-11	24.63994662715421	25.26191870726003	28.006392329204953	22.09174233638081
12-13	25.04509152124247	23.150471860951324	26.788242458564287	25.016194159241916
14-15	23.121380556923924	23.74277081094919	23.668491014665896	29.467357617460987
16-17	24.06626611173388	27.62898114495615	24.58253978720681	23.72221295610316
18-19	23.4633003502593	26.802788110577985	25.82861730971378	23.905294229448934
20-21	22.74416331470197	26.604385417111114	25.955261453246397	24.696189814940517
22-23	23.073864760345838	26.980245065145127	26.045444495731335	23.9004456787777
24-25	23.363226264405043	27.2065754104783	26.178876610203677	23.25132171491298
26-27	22.81107331396499	27.473245697396138	26.29175086982999	23.423930118808887
28-29	22.57252462094031	26.89238932698238	27.296952394990086	23.238133657087225
30-31	23.632999623752468	27.03183364428705	26.39395831797959	22.941208413980892
32-33	22.28180552269316	26.931565616405944	26.18294939276751	24.603679468133386
34-35	23.904518461341535	26.581888141996597	26.595658025902896	22.917935370758975
36-37	23.27983119285983	26.5952701418492	25.93315206218557	24.1917466031054
38-39	22.434825781877283	26.797745617879905	27.27639454014406	23.491034060098755
40-41	22.61577369292771	25.597438413709373	27.887893750800014	23.898894142562906
42-43	23.334134960377646	25.85518736739214	27.47537905969148	23.33529861253874
44-45	22.093681756649303	26.85185544337087	27.541707232873954	23.51275556710588
46-47	23.0988832818094	25.448490937089087	27.101070947872262	24.35155483322925
48-49	22.539748418402773	25.346477430966335	27.566143928256963	24.54763022237393
50-51	21.863860454832842	25.40078119848415	28.148745776912364	24.586612569770644
52-53	21.57546866090788	25.113553056720285	29.307937271390838	24.003041010980997
54-55	20.833834350236028	25.699257977805274	28.79670608861599	24.670201583342706
56-57	21.437381937791155	25.55205597942663	27.981761691795086	25.028800390987126
58-59	21.017885333716045	25.48281867584142	28.75908133540722	24.740214655035317
60-61	21.260700751331413	25.87749070047981	27.848329577322744	25.013478970866025
62-63	19.840851172767437	26.713768720254144	27.9778828512581	25.46749725572032
64-65	20.462435368819555	27.039591325361023	27.038427673199926	25.459545632619495
66-67	20.4347016589801	26.87105570402895	26.701744314589483	25.99249832240147
68-69	20.35169447148858	27.301413061607626	26.190707073841484	26.1561853930623
70-71	20.741905829509445	26.88948019657964	26.15114290036422	26.217471073546694
72-73	20.528957484028872	26.001807539690237	26.048741510187774	27.420493466093117
74-75	20.58190365735874	26.362345767603145	25.831332498089672	27.224418076948435
76	21.158299361154963	26.080935886644763	25.536734559305536	27.224030192894737
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	1.0
9	0.5
10	0.0
11	0.0
12	0.0
13	0.0
14	5.0
15	11.5
16	26.0
17	39.0
18	41.0
19	69.5
20	118.5
21	141.0
22	174.5
23	313.5
24	540.5
25	662.0
26	786.5
27	1002.5
28	1446.0
29	1798.0
30	1958.0
31	2332.0
32	3384.0
33	4222.0
34	4633.0
35	5694.5
36	7148.5
37	7952.0
38	8890.0
39	10891.5
40	13178.5
41	15173.0
42	15944.0
43	16567.0
44	17463.5
45	17867.5
46	17998.0
47	17352.5
48	15726.5
49	14187.0
50	13628.0
51	12556.5
52	10229.0
53	8508.5
54	8044.0
55	7409.5
56	6139.0
57	4906.0
58	4309.0
59	3804.5
60	3083.0
61	2631.0
62	2396.0
63	2270.0
64	2075.5
65	1895.5
66	1767.5
67	1751.0
68	1706.5
69	1637.0
70	1574.0
71	1536.0
72	1465.5
73	1318.0
74	1164.0
75	1087.0
76	1043.5
77	929.5
78	761.0
79	663.0
80	587.0
81	461.0
82	331.0
83	251.0
84	212.0
85	144.0
86	97.5
87	80.0
88	63.5
89	31.0
90	13.0
91	7.0
92	3.0
93	2.5
94	1.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	257809.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.34621754865036
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.32186931013555	24.28154176153664
2	17.817115112279993	13.664379443696689
3	6.641715557353834	7.64054008975637
4	2.975925551284645	4.56461954392593
5	1.5820352012947605	3.033253299923587
6	1.1288691078292536	2.5972716235662836
7	0.8001213837750354	2.147714005329527
8	0.6564839166498078	2.0138940068034863
9	0.6119765324701598	2.112028672389249
>10	4.239328343111471	30.82630939959427
>50	0.20837548047744286	5.219367826569282
>100	0.01517297187942545	0.8192111214115876
>500	0.0	0.0
>1k	0.0010115314586283633	1.0798692054970926
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGT	2784	1.0798692054970926	No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCG	293	0.11365002773370983	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	3.878840536986684E-4	0.0
2	0.0	0.0	0.0	3.878840536986684E-4	0.0
3	0.0	0.0	0.0	3.878840536986684E-4	0.0
4	0.0	0.0	0.0	7.757681073973368E-4	0.0
5	0.0	0.0	0.0	7.757681073973368E-4	0.0
6	0.0	0.0	0.0	7.757681073973368E-4	0.0
7	0.0	0.0	0.0	0.0011636521610960053	0.0
8	0.0	0.0	0.0	0.0015515362147946736	0.0
9	0.0	0.0	0.0	0.001939420268493342	0.0
10	0.0	0.0	0.0	0.0023273043221920106	0.0
11	0.0	0.0	0.0	0.0023273043221920106	0.0
12	0.0	0.0	0.0	0.0023273043221920106	0.0
13	0.0	0.0	0.0	0.0023273043221920106	0.0
14	0.0	0.0	0.0	0.0023273043221920106	0.0
15	0.0	0.0	0.0	0.0027151883758906787	0.0
16	0.0	0.0	0.0	0.0031030724295893473	0.0
17	0.0	0.0	0.0	0.0034909564832880154	0.0
18	0.0	0.0	0.0	0.0034909564832880154	0.0
19	0.0	0.0	0.0	0.0034909564832880154	0.0
20	0.0	0.0	0.0	0.0034909564832880154	0.0
21	0.0	0.0	0.0	0.0034909564832880154	0.0
22	0.0	0.0	0.0	0.0034909564832880154	0.0
23	0.0	0.0	0.0	0.004266724590685353	0.0
24	0.0	0.0	0.0	0.004654608644384021	0.0
25	0.0	0.0	0.0	0.004654608644384021	0.0
26	0.0	0.0	0.0	0.004654608644384021	0.0
27	0.0	0.0	0.0	0.013188057825754725	0.0
28	0.0	0.0	0.0	0.02482457943671478	0.0
29	0.0	0.0	0.0	0.035685332940277495	0.0
30	0.0	0.0	0.0	0.047709738604936214	0.0
31	0.0	0.0	0.0	0.06594028912877363	0.0
32	0.0	0.0	0.0	0.0810677672230217	0.0
33	0.0	0.0	0.0	0.10783176692822981	0.0
34	0.0	0.0	0.0	0.14390498392220596	0.0
35	0.0	0.0	0.0	0.2098452730509796	0.0
36	0.0	0.0	0.0	0.3118587791737294	0.0
37	0.0	0.0	0.0	0.4763216179419648	0.0
38	0.0	0.0	0.0	0.7051732096241792	0.0
39	0.0	0.0	0.0	0.966219177763383	0.0
40	0.0	0.0	0.0	1.2804052612593044	0.0
41	0.0	0.0	0.0	1.6128218952790632	0.0
42	0.0	0.0	0.0	1.988681543313073	0.0
43	0.0	0.0	0.0	2.4273784080462666	0.0
44	0.0	0.0	0.0	2.990973938070432	0.0
45	0.0	0.0	0.0	3.65270413368036	0.0
46	0.0	0.0	0.0	4.342749865210291	0.0
47	0.0	0.0	0.0	5.082832639667351	0.0
48	0.0	0.0	0.0	5.823691182231808	0.0
49	0.0	0.0	0.0	6.505203464580368	0.0
50	0.0	0.0	0.0	7.150254645881253	0.0
51	0.0	0.0	0.0	7.768153943423232	0.0
52	0.0	0.0	0.0	8.400017066898362	0.0
53	0.0	0.0	0.0	9.038474219286371	0.0
54	0.0	0.0	0.0	9.725416878386712	0.0
55	0.0	0.0	0.0	10.50079710173035	0.0
56	0.0	0.0	0.0	11.274625788859193	0.0
57	0.0	0.0	0.0	12.088406533519	0.0
58	0.0	0.0	0.0	12.823446815277977	0.0
59	0.0	0.0	0.0	13.52202599598928	0.0
60	0.0	0.0	0.0	14.257842045855654	0.0
61	0.0	0.0	0.0	14.912978212552703	0.0
62	0.0	0.0	0.0	15.573156871947837	0.0
63	0.0	0.0	0.0	16.24264474863174	0.0
64	0.0	0.0	0.0	16.910581089100845	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGCG	15	0.0022229417	70.0	4
AACTCGT	15	0.0022229417	70.0	37
GCATATA	15	0.0022229417	70.0	1
CGTATGC	425	0.0	58.47059	41
TATGCCG	420	0.0	58.333332	43
TCGTATG	425	0.0	57.647057	40
ATGCCGT	415	0.0	57.3494	44
CTCGTAT	410	0.0	57.19512	39
CCGTCTT	405	0.0	57.037037	47
GCCGTCT	420	0.0	56.666664	46
TCTCGTA	360	0.0	56.388885	38
CCAACGT	25	2.3875473E-4	56.000004	22
ATCTCGT	365	0.0	55.616444	37
CGTCTTC	405	0.0	55.30864	48
TGCCGTC	435	0.0	54.712646	45
CATCTCG	375	0.0	54.133335	36
CAACTCG	20	0.006945953	52.5	36
GTATGCC	470	0.0	52.12766	42
CTTGAAA	440	0.0	50.909096	57
GCATCTC	400	0.0	50.750004	35
>>END_MODULE