FastQCFastQC Report
Wed 25 May 2016
SRR1781156_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781156_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences214993
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGA150.002222437970.022
CACGGTT252.3866518E-455.99999649
TAACCGG200.00694438952.522
TACCGAA200.00694438952.511
GCACAAG350.001253492940.01
ACGGTTA350.001253492940.050
AAGCGGA459.176954E-538.8888945
TGACTAC459.176954E-538.8888934
GGTCGGA654.8455695E-737.69230725
TGCCATG400.00241507535.019
TTACTCG501.7058704E-435.019
CTAATGC400.00241507535.03
GTCGGAG708.656243E-735.026
ACAAGGA751.4843736E-632.6666683
CAACGGC552.985423E-431.8181848
ACGGCTA552.985423E-431.8181850
AGCCTTA552.985423E-431.818186
GTCTAAC450.00430074831.11111321
CCCACGA450.00430074831.1111132
GTCCCTA450.00430074831.11111312
Produced by FastQC (version 0.11.5)