FastQCFastQC Report
Wed 25 May 2016
SRR1781156_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781156_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences195865
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGT28041.4315982947438288No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCG3100.1582722793761009No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTAT150.002222141770.0000139
ATATCGT150.002222141770.0000137
ATCGATA207.912231E-570.024
AATCGAT207.912231E-570.023
TAATCGA252.3861259E-455.99999622
TCGTATG3900.047.56410240
CGTATGC3950.046.9620341
TATGCCG3950.046.9620343
CCGATCA305.8673974E-446.66666835
CGTCTTC3900.046.66666848
CCGTCTT3900.046.66666847
GCGCTAA305.8673974E-446.66666811
CGATCAA305.8673974E-446.66666836
TATCGTA305.8673974E-446.66666838
GTTGCGG305.8673974E-446.66666833
CTCGTAT3750.046.66666439
TCTCGTA3500.046.038
GCCGTCT4000.045.49999646
CTTGAAA3850.045.45454457
GTATGCC4200.044.16666842
Produced by FastQC (version 0.11.5)