##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781156_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	195865
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.209016414367035	34.0	34.0	34.0	32.0	34.0
2	33.260965460904195	34.0	34.0	34.0	32.0	34.0
3	33.32730196819238	34.0	34.0	34.0	33.0	34.0
4	33.393796747759936	34.0	34.0	34.0	34.0	34.0
5	33.38384601638884	34.0	34.0	34.0	33.0	34.0
6	36.77230235110918	38.0	37.0	38.0	34.0	38.0
7	36.68928598779772	38.0	37.0	38.0	34.0	38.0
8	36.4536696193807	38.0	37.0	38.0	34.0	38.0
9	36.4238174252674	38.0	37.0	38.0	34.0	38.0
10-11	36.347818650601184	38.0	37.0	38.0	34.0	38.0
12-13	36.27655528042274	38.0	37.0	38.0	34.0	38.0
14-15	36.29172389145585	38.0	37.0	38.0	34.0	38.0
16-17	36.22540525361856	38.0	37.0	38.0	34.0	38.0
18-19	36.230120235876754	38.0	37.0	38.0	34.0	38.0
20-21	36.16517499297986	38.0	37.0	38.0	33.0	38.0
22-23	36.2311234779057	38.0	37.0	38.0	34.0	38.0
24-25	36.23304827304521	38.0	37.0	38.0	34.0	38.0
26-27	36.01143389579558	38.0	37.0	38.0	32.0	38.0
28-29	36.00443417660123	38.0	37.0	38.0	32.0	38.0
30-31	35.99815178822148	38.0	37.0	38.0	32.0	38.0
32-33	35.954813264238126	38.0	37.0	38.0	32.0	38.0
34-35	35.963194036708956	38.0	37.0	38.0	32.0	38.0
36-37	35.91494907206494	38.0	36.5	38.0	32.0	38.0
38-39	35.83726801623567	38.0	36.0	38.0	31.0	38.0
40-41	35.9046613739055	38.0	36.5	38.0	32.0	38.0
42-43	35.913261685344494	38.0	36.0	38.0	32.0	38.0
44-45	35.87935823143492	38.0	36.0	38.0	32.0	38.0
46-47	35.90077859750338	38.0	36.5	38.0	32.0	38.0
48-49	35.8662343961402	38.0	36.0	38.0	31.5	38.0
50-51	35.77641743037296	38.0	36.0	38.0	31.0	38.0
52-53	35.81437724963623	38.0	36.0	38.0	31.0	38.0
54-55	35.83156000306333	38.0	36.0	38.0	31.5	38.0
56-57	35.7649171623312	38.0	36.0	38.0	31.0	38.0
58-59	35.77104893676767	38.0	36.0	38.0	31.0	38.0
60-61	35.76569065427718	38.0	36.0	38.0	31.0	38.0
62-63	35.737467132974245	38.0	36.0	38.0	31.0	38.0
64-65	35.67818140045439	38.0	36.0	38.0	31.0	38.0
66-67	35.64643759732469	38.0	36.0	38.0	31.0	38.0
68-69	35.548638092563756	38.0	36.0	38.0	31.0	38.0
70-71	35.44469404947285	38.0	36.0	38.0	31.0	38.0
72-73	35.32492788400174	38.0	36.0	38.0	30.0	38.0
74-75	35.34223572358512	38.0	36.0	38.0	30.0	38.0
76	34.38803767901361	37.0	34.0	38.0	25.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	4.0
21	13.0
22	21.0
23	63.0
24	128.0
25	244.0
26	554.0
27	952.0
28	1716.0
29	2718.0
30	4173.0
31	6201.0
32	8826.0
33	12270.0
34	16557.0
35	22892.0
36	34692.0
37	83841.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.110637428841294	19.514461491333314	10.714522758022108	27.66037832180328
2	22.126975212518825	19.802925484389757	32.73887626681643	25.331223036274984
3	20.041355014933757	22.435861435172185	28.834656523625966	28.688127026268095
4	14.221019579812626	17.48551298087969	36.12743471268476	32.16603272662293
5	13.696168279171877	34.468128557935316	36.63850100834758	15.197202154545222
6	30.86207336685983	38.07775763919026	16.844254971536518	14.215914022413397
7	29.31508947489342	28.5701886503459	21.61948280703546	20.49523906772522
8	25.755494856150918	35.394787225895385	18.65621729252291	20.193500625430783
9	24.223827636382204	15.936997421693514	20.43039848875501	39.408776453169274
10-11	25.0317820948102	25.374875552038397	27.151354249100145	22.441988104051262
12-13	24.902100936869783	23.06180277231767	26.680366579021264	25.355729711791287
14-15	22.67403568784622	23.756158578612823	23.301508692211474	30.26829704132949
16-17	23.960636152451944	27.555459117249125	24.72621448446634	23.75769024583259
18-19	23.739054961325404	26.512649018456592	25.701120669849132	24.047175350368878
20-21	22.521379521609273	26.3456972915018	25.5476986700023	25.58522451688663
22-23	23.012278865545145	26.948918898220715	25.945676869272205	24.09312536696194
24-25	23.71403773006918	27.30988180634621	25.878794067342305	23.097286396242307
26-27	22.98292191049958	27.321879866234394	26.206060296632884	23.48913792663314
28-29	23.559849896612462	26.066678579633933	26.836596635437676	23.536874888315932
30-31	22.979603298190078	27.007888086181808	26.13458249304368	23.877926122584434
32-33	22.414928649835346	26.45572205345519	27.778571975595433	23.350777321114034
34-35	24.00913894774462	26.720955760345134	26.585403211395604	22.68450208051464
36-37	23.354606489163455	26.628545171419088	26.551451254690733	23.465397084726725
38-39	22.078727695096113	26.70257575370791	27.335664871212316	23.883031679983663
40-41	22.33885584458683	25.45503280320629	27.97922038138514	24.226890970821742
42-43	23.296403134812245	25.477241977892934	27.583794960814846	23.642559926479972
44-45	21.83723483011258	26.264774206724017	27.96926454445664	23.928726418706763
46-47	22.837158246751592	24.799479233145277	27.498021596507797	24.865340923595333
48-49	22.19462384805861	24.726725040206265	28.14617210833993	24.932479003395194
50-51	21.056850381640416	24.90669593852909	28.902305159165753	25.134148520664745
52-53	20.763536109054705	24.830367855410614	30.095473923365585	24.310622112169096
54-55	20.029101677175607	25.29727107957011	29.433283128685574	25.240344114568707
56-57	20.670359686518776	25.349092487172285	28.039721236566002	25.940826589742933
58-59	20.080157251167897	25.172695479028924	29.37610088581421	25.371046383988972
60-61	20.491154621805833	25.742986240522807	27.93224925331223	25.833609884359127
62-63	19.06389605085135	26.876164705281695	27.834222551247034	26.225716692619915
64-65	19.556327062006996	27.0456692109361	26.718147703775557	26.67985602328134
66-67	19.75238046613739	27.019375590330075	26.209378908942384	27.01886503459015
68-69	19.749061853827893	27.238404002757	25.667168713144257	27.345365430270853
70-71	20.204988129579046	26.73346437597325	25.597733132514744	27.463814361932965
72-73	20.33722206621908	25.803231817833712	25.441503076098332	28.41804303984888
74-75	20.333137620299695	26.036300513108518	25.33326525923468	28.297296607357108
76	21.030301483164422	25.984734383376306	25.00855180864371	27.976412324815563
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	1.0
12	2.0
13	1.0
14	3.0
15	9.0
16	25.5
17	39.0
18	49.5
19	69.5
20	100.5
21	122.0
22	157.5
23	262.0
24	443.0
25	555.0
26	673.5
27	887.0
28	1144.5
29	1307.0
30	1511.0
31	1923.0
32	2698.5
33	3266.0
34	3587.5
35	4465.5
36	5623.0
37	6224.0
38	6786.0
39	8369.5
40	10479.5
41	12053.5
42	12539.0
43	12702.5
44	12672.5
45	12485.0
46	12491.0
47	12410.0
48	11526.5
49	10285.0
50	9846.0
51	9176.0
52	7756.0
53	6291.5
54	5577.0
55	5174.0
56	4325.0
57	3509.5
58	3140.0
59	2742.5
60	2190.0
61	1959.5
62	1884.0
63	1780.0
64	1639.5
65	1638.5
66	1647.5
67	1621.0
68	1580.0
69	1477.0
70	1379.5
71	1344.0
72	1339.5
73	1293.5
74	1148.0
75	1044.0
76	1008.0
77	862.5
78	690.5
79	628.0
80	533.0
81	391.0
82	306.0
83	268.0
84	240.5
85	167.0
86	98.5
87	76.0
88	53.5
89	29.0
90	18.5
91	7.5
92	5.0
93	2.5
94	1.5
95	2.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	195865.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.332729175707755
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.92226979436772	26.636714063257855
2	18.523789422902972	15.68325121895183
3	7.0964240487245975	9.01232992111914
4	3.0368449617077733	5.14231741250351
5	1.639027920159199	3.4692262527761466
6	1.104745824036664	2.806014346616292
7	0.8611228366399324	2.551757588134685
8	0.6464451546764759	2.189263012789421
9	0.5379002593016945	2.049370740050545
>10	3.5445938611831393	26.468230669083297
>50	0.07598142676234698	1.9253056952492786
>100	0.009648435144425014	0.6346207847241723
>500	0.0	0.0
>1k	0.0012060543930531267	1.4315982947438288
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGT	2804	1.4315982947438288	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCG	310	0.1582722793761009	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.001021111479845812	0.0
2	0.0	0.0	0.0	0.001021111479845812	0.0
3	0.0	0.0	0.0	0.001021111479845812	0.0
4	0.0	0.0	0.0	0.0030633344395374364	0.0
5	0.0	0.0	0.0	0.004595001659306154	0.0
6	0.0	0.0	0.0	0.004595001659306154	0.0
7	0.0	0.0	0.0	0.004595001659306154	0.0
8	0.0	0.0	0.0	0.004595001659306154	0.0
9	0.0	0.0	0.0	0.006637224618997779	0.0
10	0.0	0.0	0.0	0.006637224618997779	0.0
11	0.0	0.0	0.0	0.006637224618997779	0.0
12	0.0	0.0	0.0	0.006637224618997779	0.0
13	0.0	0.0	0.0	0.007147780358920685	0.0
14	0.0	0.0	0.0	0.007147780358920685	0.0
15	0.0	0.0	0.0	0.007147780358920685	0.0
16	0.0	0.0	0.0	0.007658336098843591	0.0
17	0.0	0.0	0.0	0.007658336098843591	0.0
18	0.0	0.0	0.0	0.007658336098843591	0.0
19	0.0	0.0	0.0	0.009700559058535216	0.0
20	0.0	0.0	0.0	0.009700559058535216	0.0
21	0.0	0.0	0.0	0.009700559058535216	0.0
22	0.0	0.0	0.0	0.009700559058535216	0.0
23	0.0	0.0	0.0	0.010721670538381028	0.0
24	0.0	0.0	0.0	0.01174278201822684	0.0
25	0.0	0.0	0.0	0.012253337758149746	0.0
26	0.0	0.0	0.0	0.013785004977918465	0.0
27	0.0	0.0	0.0	0.02859112143568274	0.0
28	0.0	0.0	0.0	0.0505450182523677	0.0
29	0.0	0.0	0.0	0.0668828019299007	0.0
30	0.0	0.0	0.0	0.08322058560743369	0.0
31	0.0	0.0	0.0	0.10211114798458122	0.0
32	0.0	0.0	0.0	0.1337656038598014	0.0
33	0.0	0.0	0.0	0.1766522860133255	0.0
34	0.0	0.0	0.0	0.23077119444515354	0.0
35	0.0	0.0	0.0	0.3374773440890409	0.0
36	0.0	0.0	0.0	0.49268628902560435	0.0
37	0.0	0.0	0.0	0.7525591606463635	0.0
38	0.0	0.0	0.0	1.0833992801164067	0.0
39	0.0	0.0	0.0	1.4550838587802823	0.0
40	0.0	0.0	0.0	1.9273479182089706	0.0
41	0.0	0.0	0.0	2.451178107369872	0.0
42	0.0	0.0	0.0	3.104178898731269	0.0
43	0.0	0.0	0.0	3.843463610139637	0.0
44	0.0	0.0	0.0	4.706813366349271	0.0
45	0.0	0.0	0.0	5.625813698210502	0.0
46	0.0	0.0	0.0	6.641819620657086	0.0
47	0.0	0.0	0.0	7.672631659561433	0.0
48	0.0	0.0	0.0	8.587036989763357	0.0
49	0.0	0.0	0.0	9.438643963954764	0.0
50	0.0	0.0	0.0	10.264212595410104	0.0
51	0.0	0.0	0.0	11.084165113726291	0.0
52	0.0	0.0	0.0	11.902075409082787	0.0
53	0.0	0.0	0.0	12.729686263497817	0.0
54	0.0	0.0	0.0	13.644091593699741	0.0
55	0.0	0.0	0.0	14.614658055293187	0.0
56	0.0	0.0	0.0	15.566333954509483	0.0
57	0.0	0.0	0.0	16.50933040614709	0.0
58	0.0	0.0	0.0	17.387996834554414	0.0
59	0.0	0.0	0.0	18.198248793812063	0.0
60	0.0	0.0	0.0	19.01054297602941	0.0
61	0.0	0.0	0.0	19.766165471115308	0.0
62	0.0	0.0	0.0	20.55344242207643	0.0
63	0.0	0.0	0.0	21.29987491384372	0.0
64	0.0	0.0	0.0	22.103489648482373	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGTAT	15	0.0022221417	70.00001	39
ATATCGT	15	0.0022221417	70.00001	37
ATCGATA	20	7.912231E-5	70.0	24
AATCGAT	20	7.912231E-5	70.0	23
TAATCGA	25	2.3861259E-4	55.999996	22
TCGTATG	390	0.0	47.564102	40
CGTATGC	395	0.0	46.96203	41
TATGCCG	395	0.0	46.96203	43
CCGATCA	30	5.8673974E-4	46.666668	35
CGTCTTC	390	0.0	46.666668	48
CCGTCTT	390	0.0	46.666668	47
GCGCTAA	30	5.8673974E-4	46.666668	11
CGATCAA	30	5.8673974E-4	46.666668	36
TATCGTA	30	5.8673974E-4	46.666668	38
GTTGCGG	30	5.8673974E-4	46.666668	33
CTCGTAT	375	0.0	46.666664	39
TCTCGTA	350	0.0	46.0	38
GCCGTCT	400	0.0	45.499996	46
CTTGAAA	385	0.0	45.454544	57
GTATGCC	420	0.0	44.166668	42
>>END_MODULE