FastQCFastQC Report
Wed 25 May 2016
SRR1781155_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781155_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences241377
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGTAT150.002222769470.05
TATACCG252.3872411E-455.9999966
CGTCATA200.006945417752.523
ACATGTA305.870125E-446.66666453
TTACTCG350.00125379940.019
TAACCGG400.00241566135.022
TAACACG400.00241566135.04
TGTTATC400.00241566135.020
GGTACAA450.004301785531.11110930
ATTCGTT450.004301785531.11110968
CATACGG450.004301785531.11110933
TAAGGCC450.004301785531.11110963
TGATCCG500.007199264627.9999985
CCATACG500.007199264627.99999832
ATCCATG500.007199264627.99999815
CGGCTTT500.007199264627.99999826
ATACCGC500.007199264627.9999987
GTAACAC500.007199264627.9999983
GACGGTG657.9410314E-426.9230777
ATAGGCG700.001223748225.032
Produced by FastQC (version 0.11.5)