##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781155_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	241377
Sequences flagged as poor quality	0
Sequence length	76
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.51776267001413	34.0	33.0	34.0	23.0	34.0
2	31.710892089967146	34.0	34.0	34.0	24.0	34.0
3	31.646279471532086	34.0	34.0	34.0	23.0	34.0
4	31.803833836695294	34.0	34.0	34.0	23.0	34.0
5	31.81588966637252	34.0	34.0	34.0	23.0	34.0
6	34.68763801024953	38.0	35.0	38.0	26.0	38.0
7	34.72934040940106	38.0	35.0	38.0	26.0	38.0
8	34.453261909792566	38.0	34.0	38.0	25.0	38.0
9	34.706082186786645	38.0	35.0	38.0	26.0	38.0
10-11	34.59227888324074	38.0	35.0	38.0	26.0	38.0
12-13	34.55078984327422	38.0	35.0	38.0	26.0	38.0
14-15	34.423629840457046	38.0	34.0	38.0	25.0	38.0
16-17	34.39405784312507	38.0	34.0	38.0	25.0	38.0
18-19	34.457899882756024	38.0	34.0	38.0	25.0	38.0
20-21	34.38700041843258	38.0	34.0	38.0	24.5	38.0
22-23	34.43250392539471	38.0	34.0	38.0	25.5	38.0
24-25	34.434138298180855	38.0	34.0	38.0	25.0	38.0
26-27	34.35723370495118	38.0	34.0	38.0	24.5	38.0
28-29	34.44311181264163	38.0	34.0	38.0	25.0	38.0
30-31	34.49939720851614	38.0	34.0	38.0	25.5	38.0
32-33	34.45430591978523	38.0	34.0	38.0	25.5	38.0
34-35	34.47273766763196	38.0	34.0	38.0	25.0	38.0
36-37	34.31889119510144	37.5	34.0	38.0	24.5	38.0
38-39	34.32877614685741	37.0	34.0	38.0	24.5	38.0
40-41	34.32701127282218	37.0	34.0	38.0	24.5	38.0
42-43	34.38752035198051	37.5	34.0	38.0	25.0	38.0
44-45	34.40069476379274	37.5	34.0	38.0	25.0	38.0
46-47	34.40953984845284	37.5	34.0	38.0	25.0	38.0
48-49	34.35259780343612	37.0	34.0	38.0	24.5	38.0
50-51	34.23225079440046	37.0	34.0	38.0	24.0	38.0
52-53	34.31868405026162	37.0	34.0	38.0	24.0	38.0
54-55	34.28623273965623	37.0	34.0	38.0	24.0	38.0
56-57	34.26012627549435	37.0	34.0	38.0	24.0	38.0
58-59	34.261597003857034	37.0	34.0	38.0	24.0	38.0
60-61	34.22827154202761	37.0	34.0	38.0	24.0	38.0
62-63	34.191720835042275	37.0	34.0	38.0	24.0	38.0
64-65	34.2093737182913	37.0	34.0	38.0	24.0	38.0
66-67	34.08456480940603	37.0	34.0	38.0	24.0	38.0
68-69	33.86973282458561	37.0	34.0	38.0	23.5	38.0
70-71	34.026756898958894	37.0	34.0	38.0	24.0	38.0
72-73	34.01353070093671	37.0	34.0	38.0	24.0	38.0
74-75	34.0141065635914	37.0	34.0	38.0	24.0	38.0
76	32.57468192909847	37.0	31.0	38.0	21.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	4.0
13	17.0
14	91.0
15	289.0
16	745.0
17	1186.0
18	1561.0
19	1796.0
20	1612.0
21	1658.0
22	1824.0
23	2052.0
24	2451.0
25	2792.0
26	3464.0
27	4119.0
28	4908.0
29	6055.0
30	7280.0
31	8924.0
32	11050.0
33	13930.0
34	18299.0
35	24325.0
36	37612.0
37	83331.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.511059462997714	17.72372678424208	9.63927797594634	26.125935776813865
2	23.585097171644357	19.60211619168355	31.129726527382477	25.683060109289617
3	20.841256623456253	21.08775898283598	28.024211088877564	30.046773304830204
4	14.907385542118758	15.57936340247828	35.59452640475273	33.918724650650226
5	14.912357018274317	33.39506249559817	34.75103261702648	16.941547869101033
6	31.945048616893907	35.46112512791194	17.116792403584434	15.477033851609722
7	30.544335210065583	27.704379456203366	21.001586729472983	20.749698604258068
8	26.895271711886387	33.177560413792534	18.952924263703665	20.974243610617414
9	25.37855719476172	14.982786263811382	20.400038114650528	39.23861842677637
10-11	27.00195130439106	23.119021282060842	26.438310195254726	23.440717218293376
12-13	26.563425678502924	21.780244182337174	25.203934094797763	26.452396044362136
14-15	24.69000774721701	22.803332546182943	22.831504244397767	29.67515546220228
16-17	25.007353641813427	25.525215741350664	24.128645231318643	25.33878538551726
18-19	24.50813457785953	25.033661036469923	25.34251399263393	25.115690393036623
20-21	24.328954291419645	25.37006425632931	25.26856328482001	25.032418167431032
22-23	25.00176073113843	24.885345331162455	25.2975635623941	24.81533037530502
24-25	24.512898909175274	25.17451952754405	25.006110772774537	25.306470790506136
26-27	23.7066911926157	25.37814290508209	25.389328726432097	25.525837175870116
28-29	24.6773719119883	24.935681527237474	25.862033250889688	24.52491330988454
30-31	24.782394345774453	24.74179395717073	25.495179739577505	24.980631957477307
32-33	24.32957572593909	25.35680698658116	25.231070068813516	25.082547218666235
34-35	25.08917585354031	24.545006359346583	25.66420992886646	24.701607858246643
36-37	24.99803212402176	24.595963989941048	25.02351093931899	25.382492946718205
38-39	24.271782315630734	24.800415946838346	26.177514841927774	24.750286895603143
40-41	24.440398215240062	24.111452209614008	26.305530352933378	25.142619222212552
42-43	24.116837975449194	24.175459965116808	26.1681933241361	25.539508735297893
44-45	23.41378838911744	24.366654652265957	26.58828305928071	25.631273899335895
46-47	23.64061198871475	23.983022408928772	26.870000041428966	25.506365560927513
48-49	23.159621670664563	24.019894190415823	27.290918355932835	25.52956578298678
50-51	22.90069062089594	24.205288822050154	27.141774071266113	25.752246485787794
52-53	22.689195739444934	24.008501224226002	27.60204990533481	25.700253130994255
54-55	21.91799550081408	24.54210633158917	27.618828637359815	25.921069530236934
56-57	22.01825360328449	24.634907219826246	27.3864121270875	25.96042704980176
58-59	22.21628407014753	24.36893324550392	27.232917800784666	26.18186488356389
60-61	21.932702784440934	24.183745758709403	27.232917800784666	26.650633656064993
62-63	21.559427783094495	23.9875795954047	27.691743620974655	26.76124900052615
64-65	22.236998554129016	24.108137892176966	26.95513657059289	26.699726983101122
66-67	22.201369641680856	24.00787978970656	26.40123126892786	27.38951929968473
68-69	22.2311984986142	24.158888377931618	26.11309279674534	27.496820326708843
70-71	22.595566271848604	24.170074199281622	26.177929131607403	27.056430397262375
72-73	22.811251302425877	23.73470491120718	25.85607961006974	27.597964176297197
74-75	22.318613621016087	24.417405138020605	25.74188924379705	27.52209199716626
76	22.96366265219967	24.338275809211314	25.504915547048807	27.193145991540206
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	1.0
10	1.5
11	1.5
12	2.0
13	1.5
14	1.0
15	6.0
16	13.0
17	15.0
18	29.5
19	72.5
20	125.0
21	149.0
22	172.0
23	294.0
24	463.0
25	533.0
26	695.0
27	971.0
28	1262.5
29	1440.0
30	1714.5
31	2240.0
32	3131.5
33	3772.0
34	4088.5
35	4977.5
36	6249.0
37	6948.0
38	7747.5
39	9450.0
40	11080.0
41	12402.0
42	12997.0
43	13108.5
44	13409.0
45	13821.5
46	14045.0
47	13713.5
48	12633.0
49	11449.5
50	11015.0
51	10138.5
52	8328.5
53	7007.0
54	6619.0
55	6207.5
56	5373.0
57	4655.5
58	4361.0
59	4076.0
60	3664.5
61	3487.0
62	3436.0
63	3351.0
64	3288.0
65	3304.5
66	3354.0
67	3409.0
68	3355.5
69	3320.5
70	3294.5
71	3250.0
72	3192.0
73	3070.5
74	2848.0
75	2689.0
76	2493.0
77	2131.5
78	1713.5
79	1461.0
80	1331.0
81	1050.0
82	761.0
83	623.0
84	502.0
85	297.5
86	175.0
87	136.0
88	116.5
89	70.0
90	37.0
91	23.0
92	15.0
93	11.0
94	5.0
95	4.0
96	5.0
97	4.0
98	2.5
99	4.5
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	2.071448398148954E-4
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	241377.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.221211319970415
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.27504477229704	30.170916383408713
2	17.93566448275342	16.580162764428678
3	6.829229360403898	9.469657602593248
4	2.9059665258791165	5.3726917152567575
5	1.4876988676294842	3.4381621870591546
6	0.9455519073772274	2.6222732714930355
7	0.6700297681461478	2.1678711252907714
8	0.5397429549691225	1.995805854407447
9	0.4381030298898826	1.8224687452013622
>10	2.907845239761124	24.432634595558284
>50	0.06413637739515837	1.8803804493877034
>100	9.867134983870519E-4	0.04697530591485167
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0012428690388893722	0.0
2	0.0	0.0	0.0	0.001657158718519163	0.0
3	0.0	0.0	0.0	0.001657158718519163	0.0
4	0.0	0.0	0.0	0.001657158718519163	0.0
5	0.0	0.0	0.0	0.0024857380777787443	0.0
6	0.0	0.0	0.0	0.0024857380777787443	0.0
7	0.0	0.0	0.0	0.0024857380777787443	0.0
8	0.0	0.0	0.0	0.0024857380777787443	0.0
9	0.0	0.0	0.0	0.0024857380777787443	0.0
10	0.0	0.0	0.0	0.0024857380777787443	0.0
11	0.0	0.0	0.0	0.0024857380777787443	0.0
12	0.0	0.0	0.0	0.0024857380777787443	0.0
13	0.0	0.0	0.0	0.003314317437038326	0.0
14	0.0	0.0	0.0	0.0037286071166681167	4.1428967962979074E-4
15	0.0	0.0	0.0	0.0037286071166681167	4.1428967962979074E-4
16	0.0	0.0	0.0	0.0037286071166681167	4.1428967962979074E-4
17	0.0	0.0	0.0	0.0037286071166681167	4.1428967962979074E-4
18	0.0	0.0	0.0	0.004142896796297907	4.1428967962979074E-4
19	0.0	0.0	0.0	0.005385765835187279	4.1428967962979074E-4
20	0.0	0.0	0.0	0.00580005551481707	4.1428967962979074E-4
21	0.0	0.0	0.0	0.00580005551481707	4.1428967962979074E-4
22	0.0	0.0	0.0	0.008700083272225606	4.1428967962979074E-4
23	0.0	0.0	0.0	0.009528662631485187	4.1428967962979074E-4
24	0.0	0.0	0.0	0.010357241990744769	4.1428967962979074E-4
25	0.0	0.0	0.0	0.01118582135000435	4.1428967962979074E-4
26	0.0	0.0	0.0	0.012428690388893722	4.1428967962979074E-4
27	0.0	0.0	0.0	0.02278593237963849	4.1428967962979074E-4
28	0.0	0.0	0.0	0.03977180924445991	4.1428967962979074E-4
29	0.0	0.0	0.0	0.06090058290557924	4.1428967962979074E-4
30	0.0	0.0	0.0	0.08161506688706878	4.1428967962979074E-4
31	0.0	0.0	0.0	0.10522957862596685	4.1428967962979074E-4
32	0.0	0.0	0.0	0.13340127684079262	4.1428967962979074E-4
33	0.0	0.0	0.0	0.17400166544451212	4.1428967962979074E-4
34	0.0	0.0	0.0	0.2539595736130617	8.285793592595815E-4
35	0.0	0.0	0.0	0.3819750846186671	8.285793592595815E-4
36	0.0	0.0	0.0	0.6019629045020859	8.285793592595815E-4
37	0.0	0.0	0.0	0.9101944261466502	8.285793592595815E-4
38	0.0	0.0	0.0	1.2706264474245683	8.285793592595815E-4
39	0.0	0.0	0.0	1.6820160992969504	8.285793592595815E-4
40	0.0	0.0	0.0	2.1273775048989756	8.285793592595815E-4
41	0.0	0.0	0.0	2.5648674065880344	8.285793592595815E-4
42	0.0	0.0	0.0	3.070715105416009	8.285793592595815E-4
43	0.0	0.0	0.0	3.654449264014384	8.285793592595815E-4
44	0.0	0.0	0.0	4.325598545014645	8.285793592595815E-4
45	0.0	0.0	0.0	5.16702088434275	8.285793592595815E-4
46	0.0	0.0	0.0	6.013828989506043	8.285793592595815E-4
47	0.0	0.0	0.0	6.931894919565659	8.285793592595815E-4
48	0.0	0.0	0.0	7.810603330060445	8.285793592595815E-4
49	0.0	0.0	0.0	8.575796368336668	8.285793592595815E-4
50	0.0	0.0	0.0	9.257717181007305	8.285793592595815E-4
51	0.0	0.0	0.0	9.963666795096467	8.285793592595815E-4
52	0.0	0.0	0.0	10.637301814174506	8.285793592595815E-4
53	0.0	0.0	0.0	11.32626555139885	0.001657158718519163
54	0.0	0.0	0.0	12.123773184686197	0.001657158718519163
55	0.0	0.0	0.0	12.98715287703468	0.001657158718519163
56	0.0	0.0	0.0	13.841832486110938	0.001657158718519163
57	0.0	0.0	0.0	14.632297194844579	0.001657158718519163
58	0.0	0.0	0.0	15.473719534172684	0.001657158718519163
59	0.0	0.0	0.0	16.170140485630363	0.001657158718519163
60	0.0	0.0	0.0	16.936990682625105	0.001657158718519163
61	0.0	0.0	0.0	17.628440157927226	0.001657158718519163
62	0.0	0.0	0.0	18.298346569888597	0.001657158718519163
63	0.0	0.0	0.0	18.916881061575875	0.001657158718519163
64	0.0	0.0	0.0	19.604601929761326	0.001657158718519163
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGGTAT	15	0.0022227694	70.0	5
TATACCG	25	2.3872411E-4	55.999996	6
CGTCATA	20	0.0069454177	52.5	23
ACATGTA	30	5.870125E-4	46.666664	53
TTACTCG	35	0.001253799	40.0	19
TAACCGG	40	0.002415661	35.0	22
TAACACG	40	0.002415661	35.0	4
TGTTATC	40	0.002415661	35.0	20
GGTACAA	45	0.0043017855	31.111109	30
ATTCGTT	45	0.0043017855	31.111109	68
CATACGG	45	0.0043017855	31.111109	33
TAAGGCC	45	0.0043017855	31.111109	63
TGATCCG	50	0.0071992646	27.999998	5
CCATACG	50	0.0071992646	27.999998	32
ATCCATG	50	0.0071992646	27.999998	15
CGGCTTT	50	0.0071992646	27.999998	26
ATACCGC	50	0.0071992646	27.999998	7
GTAACAC	50	0.0071992646	27.999998	3
GACGGTG	65	7.9410314E-4	26.923077	7
ATAGGCG	70	0.0012237482	25.0	32
>>END_MODULE