FastQCFastQC Report
Wed 25 May 2016
SRR1781155_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781155_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences219200
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGT34141.5574817518248174No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG3590.16377737226277372No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCC2890.13184306569343066No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACAG150.002222496870.049
TATGCCG6600.053.56060443
CGTATGC6750.053.4074141
TCGTATG6850.052.6277440
AGCCGAC200.006944570752.521
TTAGCGT200.006944570752.526
ATCGTAT407.4563286E-752.539
ACAGCGT200.006944570752.58
CTCGTAT6500.052.23076639
CTTGAAA6300.052.2222257
ATCTCGT5900.051.0169537
CCGTCTT6700.050.6716447
AATCTCG5950.050.58823436
ATGCCGT6850.050.58394244
TGCCGTC6950.050.35971545
TCTCGTA6050.049.75206838
CGTCTTC6850.049.56204648
GTATGCC7200.049.09722542
GCCGTCT7000.049.046
TTGAAAA6650.048.9473758
Produced by FastQC (version 0.11.5)