##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781155_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	219200
Sequences flagged as poor quality	0
Sequence length	76
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.22573905109489	34.0	34.0	34.0	32.0	34.0
2	33.245086678832116	34.0	34.0	34.0	32.0	34.0
3	33.330602189781025	34.0	34.0	34.0	33.0	34.0
4	33.386733576642335	34.0	34.0	34.0	33.0	34.0
5	33.38282390510949	34.0	34.0	34.0	34.0	34.0
6	36.77988138686131	38.0	37.0	38.0	34.0	38.0
7	36.67040145985401	38.0	37.0	38.0	34.0	38.0
8	36.45148266423357	38.0	37.0	38.0	34.0	38.0
9	36.41440693430657	38.0	37.0	38.0	34.0	38.0
10-11	36.340814324817515	38.0	37.0	38.0	34.0	38.0
12-13	36.27451414233577	38.0	37.0	38.0	34.0	38.0
14-15	36.268953010948906	38.0	37.0	38.0	34.0	38.0
16-17	36.20535812043796	38.0	37.0	38.0	34.0	38.0
18-19	36.206443886861315	38.0	37.0	38.0	33.5	38.0
20-21	36.15072308394161	38.0	37.0	38.0	32.5	38.0
22-23	36.192547901459854	38.0	37.0	38.0	33.5	38.0
24-25	36.19021669708029	38.0	37.0	38.0	33.5	38.0
26-27	35.973672445255474	38.0	36.5	38.0	31.5	38.0
28-29	35.940207572992705	38.0	36.0	38.0	32.0	38.0
30-31	35.96633895985401	38.0	37.0	38.0	32.0	38.0
32-33	35.919867700729924	38.0	36.0	38.0	31.5	38.0
34-35	35.931729014598545	38.0	36.0	38.0	32.0	38.0
36-37	35.881459854014594	38.0	36.0	38.0	31.5	38.0
38-39	35.76779197080292	38.0	36.0	38.0	31.0	38.0
40-41	35.84339416058394	38.0	36.0	38.0	31.0	38.0
42-43	35.84988138686131	38.0	36.0	38.0	31.0	38.0
44-45	35.824425182481754	38.0	36.0	38.0	31.0	38.0
46-47	35.847862682481754	38.0	36.0	38.0	31.0	38.0
48-49	35.82982892335767	38.0	36.0	38.0	31.0	38.0
50-51	35.74214872262773	38.0	36.0	38.0	31.0	38.0
52-53	35.76642563868613	38.0	36.0	38.0	31.0	38.0
54-55	35.7746875	38.0	36.0	38.0	31.0	38.0
56-57	35.71366560218978	38.0	36.0	38.0	31.0	38.0
58-59	35.71910127737226	38.0	36.0	38.0	31.0	38.0
60-61	35.704114963503656	38.0	36.0	38.0	31.0	38.0
62-63	35.68340328467153	38.0	36.0	38.0	31.0	38.0
64-65	35.61709397810219	38.0	36.0	38.0	31.0	38.0
66-67	35.57142107664234	38.0	36.0	38.0	31.0	38.0
68-69	35.472579835766425	38.0	36.0	38.0	31.0	38.0
70-71	35.31315009124087	38.0	36.0	38.0	29.5	38.0
72-73	35.20132071167883	38.0	36.0	38.0	27.0	38.0
74-75	35.207055200729926	38.0	36.0	38.0	27.5	38.0
76	34.206181569343066	37.0	34.0	38.0	24.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	2.0
21	9.0
22	43.0
23	86.0
24	161.0
25	367.0
26	695.0
27	1176.0
28	2096.0
29	3365.0
30	4914.0
31	7115.0
32	10096.0
33	13566.0
34	18824.0
35	25380.0
36	37741.0
37	93562.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.75547445255474	17.972627737226276	9.353558394160585	27.918339416058398
2	23.63229927007299	19.276003649635037	31.38184306569343	25.709854014598537
3	20.816149635036496	20.973996350364963	28.73859489051095	29.471259124087588
4	15.038321167883211	17.11131386861314	34.42244525547446	33.427919708029194
5	15.192974452554745	32.9507299270073	35.01505474452555	16.84124087591241
6	32.25821167883212	36.575273722627735	16.093978102189784	15.072536496350363
7	30.20985401459854	27.63001824817518	20.376368613138688	21.78375912408759
8	26.166058394160586	34.60903284671532	18.129105839416056	21.095802919708028
9	24.727189781021895	14.717153284671532	19.314324817518248	41.24133211678832
10-11	26.497490875912412	24.069114963503647	26.26117700729927	23.17221715328467
12-13	26.024406934306572	21.50022810218978	25.55839416058394	26.91697080291971
14-15	23.998859489051092	22.37933394160584	22.076414233576642	31.545392335766426
16-17	25.656478102189784	26.084397810218977	23.117244525547445	25.141879562043794
18-19	25.333257299270073	24.887089416058394	24.19434306569343	25.5853102189781
20-21	24.00638686131387	24.94183394160584	24.19890510948905	26.85287408759124
22-23	24.52919708029197	25.602874087591243	24.237454379562042	25.630474452554747
24-25	25.659671532846716	25.588047445255473	23.958713503649633	24.793567518248175
26-27	24.56181569343066	25.857436131386862	24.59329379562044	24.987454379562042
28-29	24.43362226277372	24.43316605839416	26.121806569343065	25.011405109489047
30-31	24.360857664233578	24.311131386861316	25.662180656934307	25.665830291970803
32-33	23.923585766423358	25.74224452554745	25.70301094890511	24.631158759124087
34-35	24.657618613138684	24.358804744525546	26.705063868613138	24.27851277372263
36-37	24.49589416058394	26.092837591240876	24.583029197080293	24.82823905109489
38-39	23.685447080291972	24.96167883211679	25.786724452554743	25.566149635036496
40-41	23.851277372262775	23.72308394160584	26.636861313868614	25.78877737226277
42-43	24.871806569343065	23.631386861313867	26.378421532846712	25.11838503649635
44-45	23.112682481751825	24.963731751824817	26.639826642335766	25.28375912408759
46-47	24.324817518248175	23.653284671532845	26.025547445255476	25.996350364963504
48-49	23.641195255474454	23.405109489051096	26.473540145985403	26.48015510948905
50-51	22.499315693430656	23.642563868613138	27.434306569343065	26.423813868613138
52-53	22.028284671532848	23.722627737226276	28.824589416058394	25.42449817518248
54-55	21.278284671532845	23.89484489051095	28.123175182481752	26.703695255474454
56-57	22.115419708029197	24.054972627737225	26.831432481751825	26.998175182481752
58-59	21.28581204379562	24.118613138686133	28.191377737226276	26.40419708029197
60-61	22.242244525547445	24.578923357664234	26.445255474452555	26.733576642335766
62-63	20.387773722627735	25.983804744525546	26.494297445255476	27.13412408759124
64-65	20.9603102189781	26.214872262773724	25.58485401459854	27.239963503649633
66-67	21.155109489051092	26.17632299270073	24.972171532846716	27.696395985401463
68-69	21.170392335766422	26.634580291970806	24.54676094890511	27.648266423357665
70-71	21.260264598540147	25.937728102189784	24.783987226277375	28.018020072992698
72-73	20.958485401459853	24.50821167883212	25.19411496350365	29.339187956204384
74-75	20.844890510948904	24.677691605839417	25.747718978102192	28.72969890510949
76	21.33211678832117	24.59352189781022	25.749087591240876	28.325273722627735
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	1.5
14	4.5
15	9.5
16	14.0
17	15.0
18	26.0
19	54.5
20	100.5
21	129.0
22	161.5
23	249.0
24	430.5
25	557.0
26	627.5
27	791.0
28	1088.5
29	1293.0
30	1471.0
31	1912.0
32	2695.0
33	3215.0
34	3533.5
35	4259.0
36	5443.5
37	6221.0
38	7272.0
39	9490.5
40	11158.5
41	11749.5
42	11840.0
43	11939.5
44	12130.0
45	12217.0
46	12213.0
47	11963.5
48	11053.0
49	10167.0
50	9942.0
51	9152.5
52	7583.0
53	6399.5
54	5996.0
55	5679.0
56	4818.5
57	4014.0
58	3753.0
59	3500.0
60	3176.5
61	3026.5
62	2947.0
63	2930.0
64	2881.5
65	2959.5
66	3079.5
67	3090.0
68	3117.0
69	3087.0
70	3034.5
71	3039.0
72	3028.0
73	2878.5
74	2640.0
75	2540.0
76	2386.5
77	2017.5
78	1661.5
79	1521.0
80	1327.5
81	996.5
82	693.5
83	528.0
84	452.5
85	300.5
86	174.0
87	124.0
88	92.0
89	47.0
90	24.0
91	14.5
92	15.0
93	8.0
94	2.5
95	3.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	219200.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.70164233576642
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.84877431469802	28.094434306569344
2	19.435429551160368	17.375912408759124
3	7.482701610434144	10.034671532846716
4	3.045333006756067	5.445255474452555
5	1.5747147551691056	3.519616788321168
6	0.8868613883616027	2.3786496350364965
7	0.6858122588941277	2.1459854014598543
8	0.5684485538750433	2.0328467153284673
9	0.4470026330292083	1.7983576642335766
>10	2.9555242585675505	23.371350364963504
>50	0.06327434531463678	1.7655109489051093
>100	0.0051027697834384505	0.47992700729927007
>500	0.0	0.0
>1k	0.00102055395668769	1.5574817518248174
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGT	3414	1.5574817518248174	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG	359	0.16377737226277372	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCC	289	0.13184306569343066	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0018248175182481751	0.0
2	0.0	0.0	0.0	0.002281021897810219	0.0
3	0.0	0.0	0.0	0.002281021897810219	0.0
4	0.0	0.0	0.0	0.002281021897810219	0.0
5	0.0	0.0	0.0	0.0031934306569343066	0.0
6	0.0	0.0	0.0	0.0031934306569343066	0.0
7	0.0	0.0	0.0	0.0031934306569343066	0.0
8	0.0	0.0	0.0	0.0031934306569343066	0.0
9	0.0	0.0	0.0	0.0031934306569343066	0.0
10	0.0	0.0	0.0	0.0036496350364963502	0.0
11	0.0	0.0	0.0	0.0036496350364963502	0.0
12	0.0	0.0	0.0	0.0036496350364963502	0.0
13	0.0	0.0	0.0	0.004562043795620438	0.0
14	0.0	0.0	0.0	0.004562043795620438	0.0
15	0.0	0.0	0.0	0.004562043795620438	0.0
16	0.0	0.0	0.0	0.004562043795620438	0.0
17	0.0	0.0	0.0	0.004562043795620438	0.0
18	0.0	0.0	0.0	0.005474452554744526	0.0
19	0.0	0.0	0.0	0.006386861313868613	0.0
20	0.0	0.0	0.0	0.006843065693430657	0.0
21	0.0	0.0	0.0	0.006843065693430657	0.0
22	0.0	0.0	0.0	0.006843065693430657	0.0
23	0.0	0.0	0.0	0.0072992700729927005	0.0
24	0.0	0.0	0.0	0.008211678832116789	0.0
25	0.0	0.0	0.0	0.009124087591240875	0.0
26	0.0	0.0	0.0	0.010492700729927007	0.0
27	0.0	0.0	0.0	0.020985401459854013	0.0
28	0.0	0.0	0.0	0.03923357664233577	0.0
29	0.0	0.0	0.0	0.06341240875912409	0.0
30	0.0	0.0	0.0	0.08622262773722628	0.0
31	0.0	0.0	0.0	0.1104014598540146	0.0
32	0.0	0.0	0.0	0.14005474452554745	0.0
33	0.0	0.0	0.0	0.1843065693430657	0.0
34	0.0	0.0	0.0	0.27007299270072993	0.0
35	0.0	0.0	0.0	0.4101277372262774	0.0
36	0.0	0.0	0.0	0.6446167883211679	0.0
37	0.0	0.0	0.0	0.9762773722627737	0.0
38	0.0	0.0	0.0	1.3608576642335766	0.0
39	0.0	0.0	0.0	1.8001824817518248	0.0
40	0.0	0.0	0.0	2.273266423357664	0.0
41	0.0	0.0	0.0	2.7317518248175183	0.0
42	0.0	0.0	0.0	3.260492700729927	0.0
43	0.0	0.0	0.0	3.8763686131386863	0.0
44	0.0	0.0	0.0	4.585766423357664	0.0
45	0.0	0.0	0.0	5.456204379562044	0.0
46	0.0	0.0	0.0	6.328923357664234	0.0
47	0.0	0.0	0.0	7.279653284671533	0.0
48	0.0	0.0	0.0	8.211678832116789	0.0
49	0.0	0.0	0.0	9.002737226277372	0.0
50	0.0	0.0	0.0	9.702554744525548	0.0
51	0.0	0.0	0.0	10.432481751824817	0.0
52	0.0	0.0	0.0	11.120437956204379	0.0
53	0.0	0.0	0.0	11.836678832116789	0.0
54	0.0	0.0	0.0	12.647810218978103	0.0
55	0.0	0.0	0.0	13.533759124087592	0.0
56	0.0	0.0	0.0	14.415602189781023	0.0
57	0.0	0.0	0.0	15.26779197080292	0.0
58	0.0	0.0	0.0	16.124543795620436	0.0
59	0.0	0.0	0.0	16.87043795620438	0.0
60	0.0	0.0	0.0	17.66788321167883	0.0
61	0.0	0.0	0.0	18.37226277372263	0.0
62	0.0	0.0	0.0	19.056569343065693	0.0
63	0.0	0.0	0.0	19.70027372262774	0.0
64	0.0	0.0	0.0	20.405565693430656	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCACAG	15	0.0022224968	70.0	49
TATGCCG	660	0.0	53.560604	43
CGTATGC	675	0.0	53.40741	41
TCGTATG	685	0.0	52.62774	40
AGCCGAC	20	0.0069445707	52.5	21
TTAGCGT	20	0.0069445707	52.5	26
ATCGTAT	40	7.4563286E-7	52.5	39
ACAGCGT	20	0.0069445707	52.5	8
CTCGTAT	650	0.0	52.230766	39
CTTGAAA	630	0.0	52.22222	57
ATCTCGT	590	0.0	51.01695	37
CCGTCTT	670	0.0	50.67164	47
AATCTCG	595	0.0	50.588234	36
ATGCCGT	685	0.0	50.583942	44
TGCCGTC	695	0.0	50.359715	45
TCTCGTA	605	0.0	49.752068	38
CGTCTTC	685	0.0	49.562046	48
GTATGCC	720	0.0	49.097225	42
GCCGTCT	700	0.0	49.0	46
TTGAAAA	665	0.0	48.94737	58
>>END_MODULE