FastQCFastQC Report
Wed 25 May 2016
SRR1781154_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781154_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104315
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACACG150.002219221370.04
ACCAGGT150.002219221370.030
TCGTGTA252.3809366E-455.99999613
GTCGTGT252.3809366E-455.99999612
TGATCAA200.00693441652.510
ACCGGGT200.00693441652.514
GCAACTC200.00693441652.525
TACGGCG200.00693441652.526
CTGGTCC200.00693441652.536
AGTCTAA200.00693441652.558
CGATGCC200.00693441652.563
GCGGACT200.00693441652.530
GCATTAC350.001250527440.043
GAAACCG350.001250527440.021
CGTGTAG350.001250527440.014
GTGGTAC350.001250527440.014
GTAACAC350.001250527440.03
TGGTACC350.001250527440.015
GGTACCC400.002409395535.016
TACCCAA601.2059934E-535.018
Produced by FastQC (version 0.11.5)