##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781154_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	104315
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.03847960504242	34.0	32.0	34.0	21.0	34.0
2	31.144456693668218	34.0	32.0	34.0	23.0	34.0
3	31.132723002444518	34.0	32.0	34.0	23.0	34.0
4	31.26442026554187	34.0	33.0	34.0	23.0	34.0
5	31.23821118726933	34.0	33.0	34.0	23.0	34.0
6	34.04416431002253	37.0	34.0	38.0	24.0	38.0
7	33.83799070124143	37.0	34.0	38.0	23.0	38.0
8	33.695029478023294	37.0	34.0	38.0	22.0	38.0
9	34.04109667833006	37.0	34.0	38.0	24.0	38.0
10-11	33.88323826870536	37.0	34.0	38.0	23.0	38.0
12-13	33.847452427742894	37.0	34.0	38.0	23.0	38.0
14-15	33.738057805684704	37.0	34.0	38.0	22.0	38.0
16-17	33.69844221828117	37.0	34.0	38.0	22.5	38.0
18-19	33.77496045631021	37.0	34.0	38.0	22.5	38.0
20-21	33.668571154675746	37.0	34.0	38.0	22.0	38.0
22-23	33.74226621291281	37.0	34.0	38.0	22.5	38.0
24-25	33.74074198341562	37.0	34.0	38.0	22.0	38.0
26-27	33.663964913962516	37.0	34.0	38.0	22.0	38.0
28-29	33.75996740641327	37.0	34.0	38.0	22.5	38.0
30-31	33.844341657479745	37.0	34.0	38.0	23.0	38.0
32-33	33.77993097828692	37.0	34.0	38.0	23.0	38.0
34-35	33.82273881992043	37.0	34.0	38.0	23.0	38.0
36-37	33.662991899535065	37.0	34.0	38.0	22.0	38.0
38-39	33.69676940037387	37.0	34.0	38.0	22.0	38.0
40-41	33.66192781479174	37.0	34.0	38.0	22.0	38.0
42-43	33.765666490916935	37.0	34.0	38.0	23.0	38.0
44-45	33.769831759574366	37.0	34.0	38.0	23.0	38.0
46-47	33.76532138235153	37.0	34.0	38.0	23.0	38.0
48-49	33.70069980347985	37.0	34.0	38.0	22.5	38.0
50-51	33.590940900158174	37.0	34.0	38.0	22.0	38.0
52-53	33.674495518381825	37.0	34.0	38.0	22.5	38.0
54-55	33.66003930403106	37.0	34.0	38.0	22.0	38.0
56-57	33.61386665388487	37.0	34.0	38.0	22.0	38.0
58-59	33.60187413123712	37.0	34.0	38.0	22.0	38.0
60-61	33.587715093706564	37.0	34.0	38.0	22.0	38.0
62-63	33.550932272444044	37.0	34.0	38.0	22.0	38.0
64-65	33.5827301922063	37.0	34.0	38.0	22.0	38.0
66-67	33.479235967981595	37.0	34.0	38.0	22.0	38.0
68-69	33.25640128457077	37.0	33.5	38.0	21.0	38.0
70-71	33.46094042084072	37.0	34.0	38.0	21.5	38.0
72-73	33.42659732540862	37.0	33.5	38.0	22.0	38.0
74-75	33.42489095527968	37.0	33.0	38.0	21.5	38.0
76	32.08330537314863	36.0	31.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	5.0
13	13.0
14	67.0
15	225.0
16	502.0
17	813.0
18	1098.0
19	1112.0
20	1021.0
21	1013.0
22	1009.0
23	1155.0
24	1257.0
25	1498.0
26	1838.0
27	2052.0
28	2548.0
29	2899.0
30	3526.0
31	4178.0
32	5056.0
33	6079.0
34	7723.0
35	10178.0
36	15358.0
37	32092.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.8754733259838	19.35100417006183	9.443512438287877	25.330010065666492
2	22.788668935435936	19.810190289028423	32.570579494799404	24.830561280736234
3	19.82936298710636	22.277716531658918	29.130997459617504	28.76192302161722
4	13.92704788381345	16.19230216172171	37.797056990845036	32.08359296361981
5	13.476489478981929	33.996069596894024	36.31309015961271	16.214350764511337
6	30.171116330345587	37.49700426592532	17.590950486507214	14.740928917221877
7	28.582658294588505	28.68043905478598	22.813593442937258	19.923309207688252
8	25.918611896659154	34.80995063030245	19.02219239802521	20.24924507501318
9	24.8708239466999	14.860758280208982	21.66514882806883	38.60326894502229
10-11	25.63725255236543	24.182044768250012	27.895796385946415	22.284906293438144
12-13	25.228394765853423	22.5724967646072	26.672098931122086	25.527009538417296
14-15	23.718544792215884	24.019556152039495	24.004697311029094	28.257201744715527
16-17	23.894933614532903	26.602597900589558	25.276326511048268	24.226141973829268
18-19	23.347553084407803	26.166419019316496	26.4228538561089	24.0631740401668
20-21	23.188899007812875	26.341849206729616	26.297272683698413	24.171979101759096
22-23	23.912189042803046	25.53419930019652	26.726741120644203	23.82687053635623
24-25	23.39165028998706	26.24023390691655	26.406557062742653	23.961558740353738
26-27	22.460336480851268	26.790490341753344	26.73153429516369	24.0176388822317
28-29	23.352825576379235	25.874514691079902	27.62210612088386	23.150553611657003
30-31	23.56372525523654	25.94353640416048	26.743037914010447	23.749700426592533
32-33	22.79442074485932	26.450174950869958	26.871974308584573	23.883429995686143
34-35	23.750659061496428	25.699563821118726	27.015769544169103	23.53400757321574
36-37	23.889181805109523	25.533240665292624	26.25796865263864	24.319608876959208
38-39	23.052772851459522	26.314528111968556	27.262138714470595	23.370560322101326
40-41	23.30872837079998	25.41293198485357	27.7639840866606	23.514355557685857
42-43	22.91329147294253	25.525571586061446	27.242966016392657	24.318170924603365
44-45	22.37118343478886	25.51694387192638	27.776446340411255	24.335426352873508
46-47	22.850980204189234	25.3578104778795	27.659972199587784	24.131237118343478
48-49	22.214925945453672	25.316109859559987	27.96625605138283	24.502708143603506
50-51	22.27052676987969	25.373148636341845	27.93941427407372	24.41691031970474
52-53	22.193356660115995	25.51694387192638	28.117241048746582	24.172458419211043
54-55	21.668504050232468	25.617600536835543	28.349230695489624	24.36466471744236
56-57	21.40987786874245	25.68734781525011	28.088271948156528	24.814502367850913
58-59	21.981977663806738	25.686142932464172	27.477352250395437	24.854527153333652
60-61	21.452914024416547	25.34115583164373	27.879153904778338	25.32677623916138
62-63	21.214590423237308	25.359248430235343	28.35881704452859	25.067344101998756
64-65	22.100369074438	25.232708622920963	27.527680582850024	25.139241719791016
66-67	21.718832382687054	25.496812538944543	27.2156449216316	25.568710156736806
68-69	21.458563006279057	25.76331304222787	27.128888462828932	25.64923548866414
70-71	21.882279633801467	25.596510568949814	27.38148876000575	25.139721037242968
72-73	21.91439390308201	25.353017303360016	26.9256578632028	25.806930930355175
74-75	21.632555241336338	25.761395772420077	26.84992570579495	25.75612328044864
76	21.99396060010545	25.420121746632795	26.76987969131956	25.816037961942197
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.5
12	1.0
13	1.5
14	1.5
15	4.0
16	15.5
17	24.0
18	30.5
19	48.0
20	76.0
21	93.0
22	111.5
23	164.5
24	255.0
25	311.0
26	386.0
27	506.5
28	633.5
29	715.0
30	868.5
31	1088.0
32	1462.5
33	1771.0
34	1973.5
35	2437.0
36	3075.0
37	3452.0
38	3805.5
39	4559.0
40	5387.0
41	6054.5
42	6294.0
43	6378.0
44	6524.5
45	6621.0
46	6655.0
47	6458.0
48	5995.5
49	5436.0
50	5142.0
51	4717.0
52	3854.0
53	3243.0
54	3070.0
55	2790.5
56	2317.5
57	1902.0
58	1680.0
59	1509.0
60	1259.5
61	1100.0
62	1019.0
63	985.5
64	922.5
65	866.0
66	814.5
67	790.0
68	832.0
69	859.5
70	842.5
71	840.0
72	849.5
73	791.5
74	728.5
75	733.0
76	670.0
77	563.5
78	465.5
79	411.0
80	342.0
81	246.5
82	184.5
83	149.0
84	124.0
85	82.5
86	51.0
87	36.0
88	24.0
89	10.0
90	4.0
91	1.0
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	9.586349038968509E-4
58-59	0.0
60-61	4.7931745194842545E-4
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	104315.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.44921631596607
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.76802241657468	42.7704548722619
2	11.845121847669187	13.37295690936107
3	4.405196569584784	7.460096822125293
4	2.165237326993292	4.889038009873939
5	1.3382015793495798	3.7770215213535927
6	0.9187399167869577	3.111728898049178
7	0.6775919164473125	2.6774672865839046
8	0.4890889020973083	2.208694818578344
9	0.3583255498004585	1.82044768250012
>10	2.0293792986329287	17.754877055073575
>50	0.005094676063513628	0.15721612423908354
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	9.586349038968509E-4	0.0
2	0.0	0.0	0.0	9.586349038968509E-4	0.0
3	0.0	0.0	0.0	9.586349038968509E-4	0.0
4	0.0	0.0	0.0	0.0019172698077937018	0.0
5	0.0	0.0	0.0	0.0019172698077937018	0.0
6	0.0	0.0	0.0	0.0019172698077937018	0.0
7	0.0	0.0	0.0	0.0019172698077937018	0.0
8	0.0	0.0	0.0	0.0019172698077937018	0.0
9	0.0	0.0	0.0	0.0028759047116905527	0.0
10	0.0	0.0	0.0	0.0038345396155874036	0.0
11	0.0	0.0	0.0	0.004793174519484254	0.0
12	0.0	0.0	0.0	0.004793174519484254	0.0
13	0.0	0.0	0.0	0.004793174519484254	0.0
14	0.0	0.0	0.0	0.004793174519484254	0.0
15	0.0	0.0	0.0	0.004793174519484254	0.0
16	0.0	0.0	0.0	0.004793174519484254	0.0
17	0.0	0.0	0.0	0.004793174519484254	0.0
18	0.0	0.0	0.0	0.004793174519484254	0.0
19	0.0	0.0	0.0	0.004793174519484254	0.0
20	0.0	0.0	0.0	0.004793174519484254	0.0
21	0.0	0.0	0.0	0.004793174519484254	0.0
22	0.0	0.0	0.0	0.012462253750659062	0.0
23	0.0	0.0	0.0	0.013420888654555912	0.0
24	0.0	0.0	0.0	0.015338158462349614	0.0
25	0.0	0.0	0.0	0.015338158462349614	0.0
26	0.0	0.0	0.0	0.016296793366246464	0.0
27	0.0	0.0	0.0	0.02013133298183387	0.0
28	0.0	0.0	0.0	0.028759047116905526	0.0
29	0.0	0.0	0.0	0.04026266596366774	0.0
30	0.0	0.0	0.0	0.0565594593299142	0.0
31	0.0	0.0	0.0	0.07669079231174807	0.0
32	0.0	0.0	0.0	0.09682212529358195	0.0
33	0.0	0.0	0.0	0.13133298183386857	0.0
34	0.0	0.0	0.0	0.18501653645209223	0.0
35	0.0	0.0	0.0	0.2732109476106025	0.0
36	0.0	0.0	0.0	0.3767435172314624	0.0
37	0.0	0.0	0.0	0.5608014187796577	0.0
38	0.0	0.0	0.0	0.8205914777357044	0.0
39	0.0	0.0	0.0	1.0957196951541006	0.0
40	0.0	0.0	0.0	1.3622201984374251	0.0
41	0.0	0.0	0.0	1.6536452092220677	0.0
42	0.0	0.0	0.0	1.9719119973158223	0.0
43	0.0	0.0	0.0	2.397545894646024	0.0
44	0.0	0.0	0.0	2.866318362651584	0.0
45	0.0	0.0	0.0	3.409864353161099	0.0
46	0.0	0.0	0.0	4.02434932655898	0.0
47	0.0	0.0	0.0	4.656089728227005	0.0
48	0.0	0.0	0.0	5.227436130949528	0.0
49	0.0	0.0	0.0	5.7633130422278676	0.0
50	0.0	0.0	0.0	6.257968652638643	0.0
51	0.0	0.0	0.0	6.724823850836409	0.0
52	0.0	0.0	0.0	7.226189905574462	0.0
53	0.0	0.0	0.0	7.766859991372286	0.0
54	0.0	0.0	0.0	8.295067823419451	0.0
55	0.0	0.0	0.0	8.875041940277045	0.0
56	0.0	0.0	0.0	9.514451421176245	0.0
57	0.0	0.0	0.0	10.080046014475387	0.0
58	0.0	0.0	0.0	10.623592004984902	0.0
59	0.0	0.0	0.0	11.189186598284044	0.0
60	0.0	0.0	0.0	11.76532617552605	0.0
61	0.0	0.0	0.0	12.259981785936827	0.0
62	0.0	0.0	0.0	12.807362316061928	0.0
63	0.0	0.0	0.0	13.387336432919522	0.0
64	0.0	0.0	0.0	13.894454297080957	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACACG	15	0.0022192213	70.0	4
ACCAGGT	15	0.0022192213	70.0	30
TCGTGTA	25	2.3809366E-4	55.999996	13
GTCGTGT	25	2.3809366E-4	55.999996	12
TGATCAA	20	0.006934416	52.5	10
ACCGGGT	20	0.006934416	52.5	14
GCAACTC	20	0.006934416	52.5	25
TACGGCG	20	0.006934416	52.5	26
CTGGTCC	20	0.006934416	52.5	36
AGTCTAA	20	0.006934416	52.5	58
CGATGCC	20	0.006934416	52.5	63
GCGGACT	20	0.006934416	52.5	30
GCATTAC	35	0.0012505274	40.0	43
GAAACCG	35	0.0012505274	40.0	21
CGTGTAG	35	0.0012505274	40.0	14
GTGGTAC	35	0.0012505274	40.0	14
GTAACAC	35	0.0012505274	40.0	3
TGGTACC	35	0.0012505274	40.0	15
GGTACCC	40	0.0024093955	35.0	16
TACCCAA	60	1.2059934E-5	35.0	18
>>END_MODULE