FastQCFastQC Report
Wed 25 May 2016
SRR1781154_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781154_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94531
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGT26192.7705197236885257No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCG2760.29196771429478163No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCC1530.1618516677068898No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTGCCTTACCTCGTATGCCGT1530.1618516677068898No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTAGG150.002218573670.000016
TACCACG150.002218573670.0000120
CACGCTA150.002218573670.0000123
TATCTAG150.002218573670.000015
CGTCTTC3700.055.8108148
CCGTCTT3850.055.45454447
TATGCCG3950.054.9367143
TGCCGTC4050.054.44444345
CGTATGC4000.054.24999641
TCGTATG4000.054.24999640
TCTCGTA3550.054.22535338
ATGCCGT4050.053.58024644
GCCGTCT4050.053.58024646
CTCGTAT3800.053.42105539
ATCTCGT3650.052.73972337
ATTTACT200.006932408552.517
ATCTACG200.006932408552.51
GCAACAG200.006932408552.527
TTAGACA200.006932408552.54
GCTTGCA200.006932408552.514
Produced by FastQC (version 0.11.5)