FastQCFastQC Report
Wed 25 May 2016
SRR1781153_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781153_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences237700
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTC601.2124989E-535.06
TCGTTGT400.002415587435.034
TTAGAGT601.2124989E-535.04
GATCAAG400.002415587435.011
CAATCGC400.002415587435.048
TCAATCG450.004301655631.11110947
GGTACTA500.00719904927.9999981
GACGTAA500.00719904927.99999868
CCATCGT500.00719904927.99999831
CTCGGGA500.00719904927.99999812
CATCGTT500.00719904927.99999832
GCGTAGC500.00719904927.99999824
AGACGTA657.9406775E-426.92307767
TCTACAC1101.0112326E-625.4545443
GGTCCTA700.001223693525.0000021
CCCCAAT700.001223693525.00000266
CTGTGCA1001.3927025E-524.4999989
GGTCCAA750.001828776223.3333341
GAGACGT750.001828776223.33333466
TATACAG750.001828776223.3333345
Produced by FastQC (version 0.11.5)