##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781153_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	237700
Sequences flagged as poor quality	0
Sequence length	76
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.18166596550274	34.0	34.0	34.0	27.0	34.0
2	32.46281026503997	34.0	34.0	34.0	31.0	34.0
3	32.451068573832565	34.0	34.0	34.0	31.0	34.0
4	32.568632730332354	34.0	34.0	34.0	31.0	34.0
5	32.60405973916702	34.0	34.0	34.0	31.0	34.0
6	35.7693605384939	38.0	37.0	38.0	31.0	38.0
7	35.68884728649558	38.0	37.0	38.0	31.0	38.0
8	35.643550694152296	38.0	37.0	38.0	31.0	38.0
9	35.80500631047539	38.0	37.0	38.0	31.0	38.0
10-11	35.721013883045856	38.0	37.0	38.0	31.0	38.0
12-13	35.67246318889356	38.0	37.0	38.0	31.0	38.0
14-15	35.592978544383676	38.0	37.0	38.0	31.0	38.0
16-17	35.58455826672276	38.0	37.0	38.0	31.0	38.0
18-19	35.60513462347497	38.0	37.0	38.0	31.0	38.0
20-21	35.570936053849394	38.0	36.5	38.0	31.0	38.0
22-23	35.60876314682373	38.0	37.0	38.0	31.0	38.0
24-25	35.61427429533025	38.0	37.0	38.0	31.0	38.0
26-27	35.548413967185525	38.0	37.0	38.0	31.0	38.0
28-29	35.63928060580564	38.0	37.0	38.0	31.0	38.0
30-31	35.70443626419857	38.0	37.0	38.0	31.0	38.0
32-33	35.66952881783761	38.0	37.0	38.0	31.0	38.0
34-35	35.68724021876315	38.0	37.0	38.0	31.0	38.0
36-37	35.60404501472445	38.0	37.0	38.0	31.0	38.0
38-39	35.59224863273033	38.0	37.0	38.0	31.0	38.0
40-41	35.583474968447625	38.0	37.0	38.0	31.0	38.0
42-43	35.65768615902398	38.0	37.0	38.0	31.0	38.0
44-45	35.66042911232647	38.0	37.0	38.0	31.0	38.0
46-47	35.629324779133356	38.0	37.0	38.0	31.0	38.0
48-49	35.5970929743374	38.0	37.0	38.0	31.0	38.0
50-51	35.53106226335717	38.0	36.5	38.0	31.0	38.0
52-53	35.57548801009676	38.0	37.0	38.0	31.0	38.0
54-55	35.542252839713925	38.0	37.0	38.0	31.0	38.0
56-57	35.464995793016406	38.0	36.0	38.0	31.0	38.0
58-59	35.473224652923854	38.0	36.0	38.0	31.0	38.0
60-61	35.48387673538073	38.0	36.0	38.0	31.0	38.0
62-63	35.45950147244426	38.0	36.0	38.0	31.0	38.0
64-65	35.48027555742533	38.0	36.0	38.0	31.0	38.0
66-67	35.384156499789654	38.0	36.0	38.0	30.5	38.0
68-69	35.138944047118216	38.0	36.0	38.0	27.5	38.0
70-71	35.34040176693311	38.0	36.0	38.0	30.0	38.0
72-73	35.35665544804375	38.0	36.0	38.0	30.5	38.0
74-75	35.38754312158183	38.0	36.0	38.0	30.5	38.0
76	34.30640302902819	37.0	34.0	38.0	25.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	14.0
14	70.0
15	157.0
16	287.0
17	452.0
18	567.0
19	675.0
20	721.0
21	859.0
22	935.0
23	1192.0
24	1397.0
25	1750.0
26	2068.0
27	2543.0
28	3075.0
29	3784.0
30	4623.0
31	5846.0
32	7637.0
33	10304.0
34	14370.0
35	21861.0
36	39802.0
37	112709.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.59150189314262	19.15271350441733	9.406394615061	24.849389987379052
2	22.179638199411023	21.99537231804796	32.125788809423646	23.699200673117375
3	19.348758939840135	22.965502734539335	30.655027345393353	27.03071098022718
4	13.379890618426588	17.469078670593184	37.80521665965503	31.3458140513252
5	13.207404291123265	34.09465713083719	36.55363904080774	16.144299537231806
6	28.36095919225915	38.10138830458561	19.715607909129155	13.822044594026083
7	27.122843920908707	30.971813209928484	23.014724442574675	18.890618426588134
8	24.301640723601178	36.040807740849814	20.896508203618005	18.761043331931006
9	24.005469078670593	17.179217501051745	22.304585612116114	36.51072780816155
10-11	24.068573832562056	25.416701724863273	28.79764408918805	21.717080353386624
12-13	24.586453512831298	23.953723180479596	27.758098443416067	23.701724863273032
14-15	22.456247370635253	25.022507362221287	25.00462768195204	27.516617585191415
16-17	22.22885990744636	28.369793857803955	26.0187210769878	23.382625157761886
18-19	21.739587715607907	28.06899453092133	27.66596550273454	22.525452250736222
20-21	21.872107698779974	28.114219604543543	27.68700042069836	22.326672275978122
22-23	21.640933950357592	27.839293226756418	28.023979806478756	22.495793016407237
24-25	21.803533866217922	27.863904080774084	27.683214135464873	22.64934791754312
26-27	21.526293647454775	27.70866638620109	27.744846445098865	23.020193521245265
28-29	21.683634833824147	27.532183424484646	28.28165755153555	22.50252419015566
30-31	21.663230963399243	28.03176272612537	27.942364324779135	22.362641985696253
32-33	21.2433740008414	28.182162389566678	28.027555742532606	22.546907867059318
34-35	21.727597812368533	27.59381573411864	28.24968447623054	22.42890197728229
36-37	22.03849389987379	27.722549432057214	27.627471602860748	22.611485065208246
38-39	21.495793016407237	28.058056373580143	27.98359276398822	22.4625578460244
40-41	21.401766933108963	27.741270509045012	28.358435002103494	22.49852755574253
42-43	21.260201935212454	27.816996213714766	28.31320992848128	22.609591922591502
44-45	21.285443836769037	27.696466133782078	28.113588557004626	22.90450147244426
46-47	21.520193521245268	27.675431215818257	28.19267984854859	22.611695414387885
48-49	21.18426588136306	27.65502734539335	28.53618005889777	22.624526714345812
50-51	21.181741691207403	27.555111485065208	28.520403870424904	22.742742953302482
52-53	21.118847286495583	27.456457719814892	28.599705511148503	22.82498948254102
54-55	20.987799747580986	27.566259991586033	28.726966764829616	22.71897349600337
56-57	20.9135464871687	27.449726546066472	28.634623474968446	23.002103491796383
58-59	20.84665544804375	27.3199411022297	28.801220025241903	23.032183424484646
60-61	20.885990744636096	27.496213714766508	28.448254101809	23.16954143878839
62-63	20.42385359697097	27.339713925115696	28.915229280605804	23.32120319730753
64-65	20.775978123685316	27.30269246949937	28.739167017248633	23.18216238956668
66-67	20.683003786285234	27.194783340344976	28.446150610012623	23.676062263357174
68-69	20.554690786705933	27.069415229280608	28.481278923012198	23.89461506100126
70-71	20.980858224652923	27.245477492637775	28.49347917543121	23.280185107278083
72-73	20.959823306689103	27.10433319310055	28.195204038704247	23.7406394615061
74-75	20.780395456457722	27.078460244005047	28.25031552376946	23.890828775767776
76	20.86705931846866	27.113167858645355	28.060580563735805	23.95919225915019
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.5
3	0.5
4	0.0
5	0.5
6	0.5
7	0.0
8	0.0
9	0.5
10	1.0
11	3.5
12	6.0
13	9.5
14	27.5
15	78.5
16	171.5
17	228.0
18	299.0
19	427.0
20	660.5
21	837.0
22	1017.5
23	1401.5
24	1825.5
25	2046.0
26	2315.5
27	2905.0
28	3632.0
29	4039.0
30	4459.5
31	5260.0
32	6299.5
33	6959.0
34	7284.0
35	8029.0
36	9011.5
37	9574.0
38	10049.5
39	11058.5
40	11983.5
41	12497.5
42	12620.0
43	12455.0
44	12359.5
45	12149.0
46	11869.0
47	11654.5
48	10844.5
49	9779.0
50	9309.0
51	8732.0
52	7608.5
53	6659.0
54	6256.0
55	6058.0
56	5522.0
57	4850.0
58	4516.0
59	4081.5
60	3443.0
61	2914.0
62	2589.0
63	2392.0
64	2052.5
65	1821.5
66	1604.0
67	1475.0
68	1411.0
69	1276.0
70	1150.0
71	1095.0
72	1062.5
73	955.5
74	812.5
75	744.0
76	712.5
77	635.5
78	540.5
79	491.0
80	429.0
81	308.5
82	222.0
83	194.0
84	169.5
85	113.0
86	70.0
87	59.0
88	45.5
89	32.5
90	25.5
91	15.0
92	12.0
93	10.5
94	5.5
95	1.5
96	1.0
97	1.0
98	1.0
99	2.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	237700.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.43838978461132
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.14473837122114	54.95683192421937
2	14.035743157334105	19.492388085513134
3	3.304310681889392	6.883380392954713
4	1.1210430501346158	3.1137369712230805
5	0.5665673304826544	1.9670761566640624
6	0.40278757055088027	1.678135219458519
7	0.2914859104545994	1.4168218586808148
8	0.2073202193175667	1.1516785759363442
9	0.1626485456689512	1.016464780085471
>10	0.7597030929684563	8.167525812207987
>50	0.0036520699776455712	0.15596022305648438
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.002524190155658393	0.0
2	0.0	0.0	0.0	0.002524190155658393	0.0
3	0.0	0.0	0.0	0.002524190155658393	0.0
4	0.0	0.0	0.0	0.002524190155658393	0.0
5	0.0	0.0	0.0	0.002524190155658393	0.0
6	0.0	0.0	0.0	0.002944888514934792	0.0
7	0.0	0.0	0.0	0.002944888514934792	0.0
8	0.0	0.0	0.0	0.002944888514934792	0.0
9	0.0	0.0	0.0	0.0033655868742111907	0.0
10	0.0	0.0	0.0	0.0037862852334875894	0.0
11	0.0	0.0	0.0	0.0037862852334875894	0.0
12	0.0	0.0	0.0	0.0037862852334875894	0.0
13	0.0	0.0	0.0	0.0037862852334875894	0.0
14	0.0	0.0	0.0	0.005469078670593185	0.0
15	0.0	0.0	0.0	0.006310475389145982	0.0
16	0.0	0.0	0.0	0.006310475389145982	0.0
17	0.0	0.0	0.0	0.006310475389145982	0.0
18	0.0	0.0	0.0	0.006310475389145982	0.0
19	0.0	0.0	0.0	0.006310475389145982	0.0
20	0.0	0.0	0.0	0.006731173748422381	0.0
21	0.0	0.0	0.0	0.006731173748422381	0.0
22	0.0	0.0	0.0	0.00715187210769878	0.0
23	0.0	0.0	0.0	0.00715187210769878	0.0
24	0.0	0.0	0.0	0.008413967185527976	0.0
25	0.0	0.0	0.0	0.008413967185527976	0.0
26	0.0	0.0	0.0	0.008834665544804375	0.0
27	0.0	0.0	0.0	0.010096760622633571	0.0
28	0.0	0.0	0.0	0.014724442574673959	0.0
29	0.0	0.0	0.0	0.018931426167437947	0.0
30	0.0	0.0	0.0	0.026924694993689526	0.0
31	0.0	0.0	0.0	0.03323517038283551	0.0
32	0.0	0.0	0.0	0.04122843920908709	0.0
33	0.0	0.0	0.0	0.04880100967606226	0.0
34	0.0	0.0	0.0	0.05931846865797223	0.0
35	0.0	0.0	0.0	0.07909129154396298	0.0
36	0.0	0.0	0.0	0.11442995372318047	0.0
37	0.0	0.0	0.0	0.1800588977702987	0.0
38	0.0	0.0	0.0	0.27471602860748845	0.0
39	0.0	0.0	0.0	0.4164913756836348	0.0
40	0.0	0.0	0.0	0.5818258308792595	0.0
41	0.0	0.0	0.0	0.7480016827934372	0.0
42	0.0	0.0	0.0	0.9255363904080774	0.0
43	0.0	0.0	0.0	1.106436684896929	0.0
44	0.0	0.0	0.0	1.3020614219604543	0.0
45	0.0	0.0	0.0	1.5473285654185949	0.0
46	0.0	0.0	0.0	1.8254101809002945	0.0
47	0.0	0.0	0.0	2.1438788388725283	0.0
48	0.0	0.0	0.0	2.4976861590239796	0.0
49	0.0	0.0	0.0	2.833403449726546	0.0
50	0.0	0.0	0.0	3.1931005469078673	0.0
51	0.0	0.0	0.0	3.5111485065208248	0.0
52	0.0	0.0	0.0	3.8287757677745056	0.0
53	0.0	0.0	0.0	4.139251156920488	0.0
54	0.0	0.0	0.0	4.46613378207825	0.0
55	0.0	0.0	0.0	4.802271771140092	0.0
56	0.0	0.0	0.0	5.153975599495162	0.0
57	0.0	0.0	0.0	5.48590660496424	0.0
58	0.0	0.0	0.0	5.86916281026504	0.0
59	0.0	0.0	0.0	6.2406394615061	0.0
60	0.0	0.0	0.0	6.659655027345393	0.0
61	0.0	0.0	0.0	7.053849389987379	0.0
62	0.0	0.0	0.0	7.425746739587716	0.0
63	0.0	0.0	0.0	7.790492217080353	0.0
64	0.0	0.0	0.0	8.135044173327724	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCTC	60	1.2124989E-5	35.0	6
TCGTTGT	40	0.0024155874	35.0	34
TTAGAGT	60	1.2124989E-5	35.0	4
GATCAAG	40	0.0024155874	35.0	11
CAATCGC	40	0.0024155874	35.0	48
TCAATCG	45	0.0043016556	31.111109	47
GGTACTA	50	0.007199049	27.999998	1
GACGTAA	50	0.007199049	27.999998	68
CCATCGT	50	0.007199049	27.999998	31
CTCGGGA	50	0.007199049	27.999998	12
CATCGTT	50	0.007199049	27.999998	32
GCGTAGC	50	0.007199049	27.999998	24
AGACGTA	65	7.9406775E-4	26.923077	67
TCTACAC	110	1.0112326E-6	25.454544	3
GGTCCTA	70	0.0012236935	25.000002	1
CCCCAAT	70	0.0012236935	25.000002	66
CTGTGCA	100	1.3927025E-5	24.499998	9
GGTCCAA	75	0.0018287762	23.333334	1
GAGACGT	75	0.0018287762	23.333334	66
TATACAG	75	0.0018287762	23.333334	5
>>END_MODULE