FastQCFastQC Report
Wed 25 May 2016
SRR1781153_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781153_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences224366
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGT6300.28079120722391093No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCA200.00694478352.53
CGTATGC1600.052.541
TATGCCG1600.052.543
ATTCCGC200.00694478352.546
CGAATGC200.00694478352.523
TCGTATG1600.052.540
TCGAACA200.00694478352.528
TGCCGTC1800.050.55555745
CGTCTTC1600.050.312548
CCGTCTT1600.050.312547
ATGCCGT1850.049.1891944
CTCGTAT1750.048.039
AATCTCG1550.047.41935336
GCCGTCT1700.047.3529446
ATCTCGT1650.044.54545637
GTATGCC1900.044.21052642
TCTCGTA1700.043.23529438
TAAACCG350.0012536140.05
GAGCGTC350.0012536140.049
TGAGCGT459.178239E-538.8888948
Produced by FastQC (version 0.11.5)