FastQCFastQC Report
Wed 25 May 2016
SRR1781152_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781152_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences810398
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAAC400.002419013335.043
GTCGTTG604.9830094E-429.1666681
GCGGGTA853.9571005E-628.823539
CGTTCAT901.9281899E-423.33333258
CGGGTAG1102.922086E-522.27272810
GTACCGT1003.9322497E-421.011
TCAACCG850.003789813520.58823625
GCTTCTA3250.020.4615381
ACTCGCA900.00527554919.44444321
TGAAGCG1451.1165039E-519.31034541
CGAACAG1651.5919686E-619.0909146
ATCGTCC1651.5919686E-619.0909129
AGCGAAC1501.4955836E-518.66666644
GCGTTTG950.00720881418.42105119
TATCCGA950.00720881418.4210512
TCGCCAG1150.001007668418.2608741
TTAATAC2306.0699676E-918.260873
TCGTCCC1351.4117912E-418.14814830
TAGTACC1752.7941187E-618.04
TCGTTCA1200.001340605417.50000257
Produced by FastQC (version 0.11.5)