##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781152_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	810398
Sequences flagged as poor quality	0
Sequence length	76
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.399997285284513	34.0	32.0	34.0	23.0	34.0
2	31.477037208877615	34.0	33.0	34.0	23.0	34.0
3	31.410297162628734	34.0	33.0	34.0	23.0	34.0
4	31.602363283226268	34.0	33.0	34.0	23.0	34.0
5	31.69805453616618	34.0	34.0	34.0	23.0	34.0
6	34.54848975441696	38.0	35.0	38.0	26.0	38.0
7	34.39109918830007	37.0	34.0	38.0	24.0	38.0
8	34.1658197576993	37.0	34.0	38.0	24.0	38.0
9	34.48712114294458	37.0	34.0	38.0	26.0	38.0
10-11	34.36013600724582	37.5	34.0	38.0	24.5	38.0
12-13	34.323243640778976	37.0	34.0	38.0	24.0	38.0
14-15	34.22194896828472	37.0	34.0	38.0	24.0	38.0
16-17	34.19030649137831	37.0	34.0	38.0	24.0	38.0
18-19	34.257108235706404	37.0	34.0	38.0	24.0	38.0
20-21	34.19981910123174	37.0	34.0	38.0	24.0	38.0
22-23	34.264302848723716	37.0	34.0	38.0	24.5	38.0
24-25	34.26176212182162	37.0	34.0	38.0	24.0	38.0
26-27	34.18316864059388	37.0	34.0	38.0	24.0	38.0
28-29	34.28482548081313	37.0	34.0	38.0	24.0	38.0
30-31	34.36567957966332	37.0	34.0	38.0	25.0	38.0
32-33	34.31175669239065	37.0	34.0	38.0	24.5	38.0
34-35	34.34534697765789	37.0	34.0	38.0	24.5	38.0
36-37	34.20351173127278	37.0	34.0	38.0	24.0	38.0
38-39	34.21074829898396	37.0	34.0	38.0	24.0	38.0
40-41	34.190331170609994	37.0	34.0	38.0	24.0	38.0
42-43	34.28073119627639	37.0	34.0	38.0	24.0	38.0
44-45	34.29286967638124	37.0	34.0	38.0	24.0	38.0
46-47	34.26568241777497	37.0	34.0	38.0	24.0	38.0
48-49	34.24006352434236	37.0	34.0	38.0	24.0	38.0
50-51	34.11412725599027	37.0	34.0	38.0	24.0	38.0
52-53	34.19621469944397	37.0	34.0	38.0	24.0	38.0
54-55	34.1593957536914	37.0	34.0	38.0	24.0	38.0
56-57	34.11801917082717	37.0	34.0	38.0	24.0	38.0
58-59	34.12201658937954	37.0	34.0	38.0	24.0	38.0
60-61	34.10093188778847	37.0	34.0	38.0	24.0	38.0
62-63	34.07430854962623	37.0	34.0	38.0	24.0	38.0
64-65	34.096228026229085	37.0	34.0	38.0	24.0	38.0
66-67	33.981572017699946	37.0	34.0	38.0	24.0	38.0
68-69	33.798685954308866	37.0	34.0	38.0	23.5	38.0
70-71	33.96408863299268	37.0	34.0	38.0	24.0	38.0
72-73	33.950531714046676	37.0	34.0	38.0	24.0	38.0
74-75	33.96467229682206	37.0	34.0	38.0	24.0	38.0
76	32.75975631726633	37.0	31.0	38.0	21.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	6.0
13	48.0
14	144.0
15	451.0
16	1086.0
17	2231.0
18	3413.0
19	4434.0
20	5320.0
21	6160.0
22	7470.0
23	8801.0
24	10253.0
25	12137.0
26	14257.0
27	16768.0
28	19617.0
29	23305.0
30	27558.0
31	32654.0
32	39476.0
33	47310.0
34	60067.0
35	78306.0
36	119853.0
37	269271.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	49.98729019568163	17.62220538550194	8.577883953316766	23.812620465499666
2	23.91874116175015	22.323228833239963	30.180602617479312	23.577427387530573
3	19.57618355425359	22.92910397113517	30.671966120350742	26.822746354260502
4	13.745221483764766	17.54927825586934	37.787358803945715	30.91814145642018
5	13.61701287515517	33.01834407291232	36.76625559293088	16.598387459001625
6	28.39382624340139	38.17901821080506	19.73141592155953	13.695739624234019
7	27.639135338438642	31.33188877563864	22.38480351629693	18.64417236962579
8	24.823605191523175	35.575482664073704	20.998941260960663	18.601970883442455
9	24.626664922667626	17.877512037295254	21.889491336355714	35.6063317036814
10-11	24.32428263643296	25.1182135197767	28.39543039346099	22.162073450329345
12-13	25.01382036974425	23.6424571630236	27.79831638281437	23.54540608441778
14-15	22.95057490270213	24.395975804481253	25.06533826588911	27.588111026927507
16-17	22.956374522148376	28.10199432871256	25.65295077233656	23.28868037680251
18-19	22.071994254674863	27.65671929101503	27.45354751615873	22.81773893815138
20-21	22.323599021715257	27.799488646319464	27.365689451356	22.51122288060928
22-23	22.429102737173586	27.48593900774681	27.631978361249658	22.452979893829944
24-25	22.43292801808494	27.537580300050095	27.316022497587607	22.713469184277354
26-27	22.096488392123376	27.30146175089277	27.442565258058387	23.159484598925467
28-29	22.124252527770306	27.4085696164107	27.86507370452543	22.602104151293563
30-31	22.284605835651124	27.56670179343977	27.725636045498632	22.423056325410478
32-33	21.879866435998114	27.94324517089134	27.6662207952142	22.51066759789634
34-35	22.189220605184122	27.3043615606159	28.121429223665405	22.384988610534577
36-37	22.578720579270925	27.361987566603073	27.29720458342691	22.762087270699087
38-39	22.190269472530783	27.5804604651048	27.727055101320587	22.502214961043833
40-41	22.107902536778226	27.047018872208472	28.217122944528487	22.627955646484814
42-43	21.826682691714442	27.268514976591753	28.19830503036779	22.706497301326014
44-45	21.822117033852503	27.03720887761322	28.296590070557926	22.84408401797635
46-47	21.838960609478306	27.2166268919716	28.2191589811426	22.725253517407495
48-49	21.51072682805239	26.95718646887085	28.5934195296632	22.938667173413556
50-51	21.633704447508784	27.005265934310014	28.402111309573385	22.958918308607814
52-53	21.337972206249276	26.804792213208817	28.763150945584766	23.09408463495714
54-55	21.0223248329833	27.160111451410295	28.75099642397933	23.066567291627077
56-57	21.11009857520362	26.822549208936614	28.840018435420195	23.227333780439572
58-59	21.096635352796223	26.7623769584537	28.6915548798922	23.449432808857882
60-61	21.00297755297712	26.8174117130246	28.584940467449904	23.59467026654837
62-63	20.793574013724136	26.817596807490652	28.643306922409632	23.74552225637558
64-65	20.861477940468756	26.673005116004727	28.653081572264494	23.812435371262023
66-67	21.029988369904892	26.505757976795337	28.343498098155536	24.120755555144235
68-69	21.116885376479292	26.57112907076968	28.10093577649953	24.2110497762515
70-71	21.49150354702695	26.72683882097293	27.88620885812756	23.89544877387256
72-73	21.404817638762236	26.503491498611176	27.977090240955977	24.11460062167061
74-75	21.234160541068142	26.661006889216026	27.960616833477914	24.144215736237918
76	21.33815730048692	26.625435896929655	27.465023358892793	24.57138344369063
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.5
2	0.5
3	0.0
4	0.0
5	0.0
6	0.5
7	1.0
8	1.0
9	2.0
10	3.0
11	5.5
12	8.0
13	18.0
14	38.0
15	92.0
16	212.0
17	288.0
18	410.5
19	692.5
20	1157.0
21	1462.0
22	1888.5
23	2914.5
24	4254.0
25	4994.0
26	5927.5
27	7878.5
28	10221.5
29	11547.0
30	12796.0
31	15496.5
32	18939.0
33	20930.0
34	22849.0
35	26794.5
36	31028.5
37	33236.0
38	34962.5
39	38140.0
40	40908.5
41	42827.5
42	43429.0
43	43573.5
44	43436.5
45	42629.0
46	42103.0
47	41155.5
48	38683.5
49	35440.0
50	33721.0
51	32111.5
52	28782.0
53	25903.0
54	24744.0
55	23356.5
56	20700.5
57	17995.0
58	16558.0
59	15231.5
60	12469.0
61	10199.0
62	9365.0
63	8551.5
64	7098.5
65	5965.5
66	5215.5
67	4959.0
68	4633.5
69	4082.5
70	3683.0
71	3509.0
72	3271.5
73	2872.0
74	2596.0
75	2482.0
76	2351.0
77	1981.5
78	1553.5
79	1364.0
80	1274.5
81	1010.5
82	722.5
83	609.0
84	522.0
85	362.5
86	210.0
87	130.0
88	115.0
89	74.0
90	39.0
91	27.0
92	24.0
93	18.0
94	9.0
95	6.5
96	7.0
97	6.0
98	4.5
99	9.0
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	6.169807921539786E-5
52-53	0.0
54-55	0.0
56-57	1.850942376461936E-4
58-59	1.2339615843079573E-4
60-61	1.2339615843079573E-4
62-63	1.2339615843079573E-4
64-65	0.0
66-67	6.169807921539786E-5
68-69	1.850942376461936E-4
70-71	1.2339615843079573E-4
72-73	1.2339615843079573E-4
74-75	1.2339615843079573E-4
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	810398.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.962349693632966
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.51507252045417	43.59726859357448
2	17.533870565058905	21.378118977400376
3	6.250830414184164	11.431959287552745
4	2.112739014733647	5.151901385102967
5	0.7323761252292718	2.23236847267474
6	0.3627145284713019	1.326715795417722
7	0.21669395790664187	0.9247120986881457
8	0.15911827046105745	0.7760178917194444
9	0.12489752627150336	0.6852642005189787
>10	0.9496211277711951	10.773567201106623
>50	0.039018010080155395	1.5178394691598023
>100	0.003047939377974325	0.20426662708389193
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.4679231686159145E-4	0.0
2	0.0	0.0	0.0	2.4679231686159145E-4	0.0
3	0.0	0.0	0.0	2.4679231686159145E-4	0.0
4	0.0	0.0	0.0	0.0011105654258771617	0.0
5	0.0	1.2339615843079573E-4	0.0	0.0012339615843079573	0.0
6	0.0	1.2339615843079573E-4	0.0	0.001357357742738753	0.0
7	0.0	1.2339615843079573E-4	0.0	0.0014807539011695487	0.0
8	0.0	1.2339615843079573E-4	0.0	0.0014807539011695487	0.0
9	0.0	1.2339615843079573E-4	0.0	0.0017275462180311402	0.0
10	0.0	1.2339615843079573E-4	0.0	0.001850942376461936	0.0
11	0.0	1.2339615843079573E-4	0.0	0.0024679231686159145	0.0
12	0.0	1.2339615843079573E-4	0.0	0.0030849039607698933	0.0
13	0.0	1.2339615843079573E-4	0.0	0.003208300119200689	0.0
14	0.0	1.2339615843079573E-4	0.0	0.003208300119200689	0.0
15	0.0	1.2339615843079573E-4	0.0	0.003578488594493076	0.0
16	0.0	1.2339615843079573E-4	0.0	0.003578488594493076	0.0
17	0.0	1.2339615843079573E-4	0.0	0.003948677069785463	0.0
18	0.0	1.2339615843079573E-4	0.0	0.004195469386647055	0.0
19	0.0	1.2339615843079573E-4	0.0	0.004935846337231829	0.0
20	0.0	1.2339615843079573E-4	0.0	0.005552827129385808	0.0
21	0.0	1.2339615843079573E-4	0.0	0.005676223287816604	0.0
22	0.0	1.2339615843079573E-4	0.0	0.0070335810305553565	0.0
23	0.0	1.2339615843079573E-4	0.0	0.007650561822709336	0.0
24	0.0	1.2339615843079573E-4	0.0	0.007897354139570927	0.0
25	0.0	1.2339615843079573E-4	0.0	0.008637731090155702	0.0
26	0.0	1.2339615843079573E-4	0.0	0.00950150419917127	0.0
27	0.0	1.2339615843079573E-4	0.0	0.012956596635233552	0.0
28	0.0	1.2339615843079573E-4	0.0	0.01900300839834254	0.0
29	0.0	1.2339615843079573E-4	0.0	0.03097243576612973	0.0
30	0.0	1.2339615843079573E-4	0.0	0.044422617035086466	0.0
31	0.0	1.2339615843079573E-4	0.0	0.05885996757148956	0.0
32	0.0	1.2339615843079573E-4	0.0	0.07280373347416948	0.0
33	0.0	1.2339615843079573E-4	0.0	0.08995579949605009	0.0
34	0.0	1.2339615843079573E-4	0.0	0.11981766983630265	0.0
35	0.0	1.2339615843079573E-4	0.0	0.15461538651378706	0.0
36	0.0	1.2339615843079573E-4	0.0	0.21619006957075412	0.0
37	0.0	1.2339615843079573E-4	0.0	0.33292283544628687	0.0
38	0.0	1.2339615843079573E-4	0.0	0.4991374608525687	0.0
39	0.0	1.2339615843079573E-4	0.0	0.7086641378680599	0.0
40	0.0	1.2339615843079573E-4	0.0	0.9581711702151289	0.0
41	0.0	1.2339615843079573E-4	0.0	1.2140948028005991	0.0
42	0.0	1.2339615843079573E-4	0.0	1.4765584317829017	0.0
43	0.0	1.2339615843079573E-4	0.0	1.7504979034992683	0.0
44	0.0	1.2339615843079573E-4	0.0	2.0357898217912678	0.0
45	0.0	1.2339615843079573E-4	0.0	2.375499445951249	0.0
46	0.0	1.2339615843079573E-4	0.0	2.760742252572193	0.0
47	0.0	1.2339615843079573E-4	0.0	3.177574475751421	0.0
48	0.0	1.2339615843079573E-4	0.0	3.645122520045706	0.0
49	0.0	1.2339615843079573E-4	0.0	4.131426780421472	0.0
50	0.0	1.2339615843079573E-4	0.0	4.636980841512442	0.0
51	0.0	1.2339615843079573E-4	0.0	5.087993800577	0.0
52	0.0	1.2339615843079573E-4	0.0	5.511489416311491	0.0
53	0.0	1.2339615843079573E-4	0.0	5.910799384993546	0.0
54	0.0	1.2339615843079573E-4	0.0	6.319610857874773	0.0
55	0.0	1.2339615843079573E-4	0.0	6.736196288737139	0.0
56	0.0	1.2339615843079573E-4	0.0	7.174005858849602	0.0
57	0.0	1.2339615843079573E-4	0.0	7.588987139652368	0.0
58	0.0	1.2339615843079573E-4	0.0	8.015814451664491	0.0
59	0.0	1.2339615843079573E-4	0.0	8.480524384314867	0.0
60	0.0	1.2339615843079573E-4	0.0	8.974725998830204	0.0
61	0.0	1.2339615843079573E-4	0.0	9.461523843839693	0.0
62	0.0	1.2339615843079573E-4	0.0	9.898716433160002	0.0
63	0.0	1.2339615843079573E-4	0.0	10.305800359823198	0.0
64	0.0	1.2339615843079573E-4	0.0	10.71695635971461	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGAAC	40	0.0024190133	35.0	43
GTCGTTG	60	4.9830094E-4	29.166668	1
GCGGGTA	85	3.9571005E-6	28.82353	9
CGTTCAT	90	1.9281899E-4	23.333332	58
CGGGTAG	110	2.922086E-5	22.272728	10
GTACCGT	100	3.9322497E-4	21.0	11
TCAACCG	85	0.0037898135	20.588236	25
GCTTCTA	325	0.0	20.461538	1
ACTCGCA	90	0.005275549	19.444443	21
TGAAGCG	145	1.1165039E-5	19.310345	41
CGAACAG	165	1.5919686E-6	19.09091	46
ATCGTCC	165	1.5919686E-6	19.09091	29
AGCGAAC	150	1.4955836E-5	18.666666	44
GCGTTTG	95	0.007208814	18.421051	19
TATCCGA	95	0.007208814	18.421051	2
TCGCCAG	115	0.0010076684	18.26087	41
TTAATAC	230	6.0699676E-9	18.26087	3
TCGTCCC	135	1.4117912E-4	18.148148	30
TAGTACC	175	2.7941187E-6	18.0	4
TCGTTCA	120	0.0013406054	17.500002	57
>>END_MODULE