FastQCFastQC Report
Wed 25 May 2016
SRR1781152_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781152_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences724870
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGT38360.5291983390125126No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCCG200.006951006652.534
ACGTAGC200.006951006652.57
TATGCCG5600.049.37543
TCGTATG5650.048.93805340
CTCGTAT5400.048.6111139
TCTCGTA4800.047.39583638
CGTATGC5850.047.26495741
CCGTCTT5950.047.05882347
CGTCTTC6150.046.09756548
GCCGTCT6200.045.72580746
ATGCCGT6450.043.95348744
GTATGCC6350.043.54330442
ATCTCGT5250.043.33333237
TATCTCG5250.043.33333236
TGCCGTC6850.041.38686445
CGGTCAT8050.034.78260428
GTCTTCT8550.032.74853549
GCGAAGT450.00430742331.11110932
TCGTGAG450.00430742331.11110926
GGTCATT9050.030.16574529
Produced by FastQC (version 0.11.5)