##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781152_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	724870
Sequences flagged as poor quality	0
Sequence length	76
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.29340985280119	34.0	34.0	34.0	33.0	34.0
2	33.32639231862265	34.0	34.0	34.0	33.0	34.0
3	33.41156069364162	34.0	34.0	34.0	34.0	34.0
4	33.46617876308856	34.0	34.0	34.0	34.0	34.0
5	33.46522548870832	34.0	34.0	34.0	34.0	34.0
6	36.92188668312939	38.0	37.0	38.0	35.0	38.0
7	36.84498323837378	38.0	37.0	38.0	34.0	38.0
8	36.63689351194007	38.0	37.0	38.0	34.0	38.0
9	36.5871811497234	38.0	37.0	38.0	34.0	38.0
10-11	36.53032750700125	38.0	37.0	38.0	34.0	38.0
12-13	36.46887993709217	38.0	37.0	38.0	34.0	38.0
14-15	36.4859809345124	38.0	37.0	38.0	34.0	38.0
16-17	36.43903044683874	38.0	37.0	38.0	34.0	38.0
18-19	36.44595858567743	38.0	37.0	38.0	34.0	38.0
20-21	36.38706043842345	38.0	37.0	38.0	34.0	38.0
22-23	36.427710486018185	38.0	37.0	38.0	34.0	38.0
24-25	36.424941023907735	38.0	37.0	38.0	34.0	38.0
26-27	36.24206547380909	38.0	37.0	38.0	34.0	38.0
28-29	36.22010774345745	38.0	37.0	38.0	34.0	38.0
30-31	36.250126229530814	38.0	37.0	38.0	34.0	38.0
32-33	36.214453626167455	38.0	37.0	38.0	34.0	38.0
34-35	36.2254680149544	38.0	37.0	38.0	34.0	38.0
36-37	36.1966952694966	38.0	37.0	38.0	34.0	38.0
38-39	36.18408542221364	38.0	37.0	38.0	34.0	38.0
40-41	36.1938402748079	38.0	37.0	38.0	34.0	38.0
42-43	36.181891235669845	38.0	37.0	38.0	34.0	38.0
44-45	36.15121401078814	38.0	37.0	38.0	34.0	38.0
46-47	36.16937726764799	38.0	37.0	38.0	34.0	38.0
48-49	36.16424945162581	38.0	37.0	38.0	34.0	38.0
50-51	36.093327079338366	38.0	37.0	38.0	33.5	38.0
52-53	36.121067915626256	38.0	37.0	38.0	34.0	38.0
54-55	36.11929104529088	38.0	37.0	38.0	34.0	38.0
56-57	36.058896767696275	38.0	37.0	38.0	32.0	38.0
58-59	36.07946666298785	38.0	37.0	38.0	33.0	38.0
60-61	36.08591540552099	38.0	37.0	38.0	33.5	38.0
62-63	36.05497537489481	38.0	37.0	38.0	32.5	38.0
64-65	36.00086222357112	38.0	37.0	38.0	32.0	38.0
66-67	35.995817870790624	38.0	37.0	38.0	32.0	38.0
68-69	35.96267123760123	38.0	37.0	38.0	32.0	38.0
70-71	35.912903693076004	38.0	37.0	38.0	32.0	38.0
72-73	35.867204464248765	38.0	37.0	38.0	32.0	38.0
74-75	35.87514588822823	38.0	37.0	38.0	32.0	38.0
76	35.08777573909804	38.0	35.0	38.0	27.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	1.0
19	3.0
20	7.0
21	26.0
22	88.0
23	181.0
24	354.0
25	757.0
26	1414.0
27	2646.0
28	4625.0
29	7749.0
30	12109.0
31	18495.0
32	27162.0
33	39218.0
34	54778.0
35	78563.0
36	123599.0
37	353094.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	49.219998068619205	17.124036034047485	8.785989211858677	24.869976685474636
2	24.251383006608084	22.581428394056864	29.589857491688164	23.577331107646888
3	20.30322678549257	22.848372811676576	30.806489439485702	26.04191096334515
4	14.109702429401134	18.15111675196932	36.99035689158056	30.748823927048992
5	13.511664160470152	32.98425924648558	36.839019410377034	16.665057182667237
6	28.89373266930622	38.34411687612951	19.45728199539228	13.30486845917199
7	27.514864734366164	31.70968587470857	21.766523652517	19.008925738408266
8	24.728847931353208	36.32361664850249	20.668119800791864	18.279415619352434
9	24.360368065998042	18.24120187068026	21.400389035275293	35.99804102804641
10-11	23.95788210299778	25.69260695021176	28.2878309214066	22.06168002538386
12-13	24.704084870390552	23.951536137514314	27.721522479892947	23.622856512202187
14-15	22.665650392484167	24.34684840040283	24.97185702263854	28.015644184474457
16-17	23.071378316111854	28.403437857822784	25.31040048560432	23.214783340461047
18-19	22.319243450549752	27.675376274366435	27.114241174279524	22.89113910080428
20-21	22.122173631133858	27.825817043055995	27.056092816642984	22.99591650916716
22-23	22.109481700166928	27.973567674203647	27.271165864223928	22.645784761405494
24-25	22.61101990701781	27.809676217804576	27.04871218287417	22.530591692303446
26-27	22.29668768192917	27.685653979334223	27.096031012457406	22.921627326279197
28-29	22.382910039041484	27.32076096403493	27.562114586063707	22.73421441085988
30-31	22.40215486914895	27.53355774138811	27.758011781422876	22.306275608040064
32-33	21.598838412404984	28.1369762854029	27.591637121138962	22.672548181053152
34-35	22.02119000648392	27.231434602066578	28.49531640156166	22.25205898988784
36-37	22.242057196462813	27.688826962075957	27.570184998689417	22.49893084277181
38-39	21.864886117510725	27.59777615296536	27.80926235049043	22.728075379033484
40-41	21.830259218894422	26.950625629423207	28.379295597831334	22.839819553851033
42-43	22.062093892698	27.101135375998453	28.190503124698225	22.64626760660532
44-45	21.617255507884174	27.450163477589086	28.422130864844732	22.51045014968201
46-47	22.05374756852953	26.999461972491616	27.966600907748973	22.980189551229877
48-49	21.688302730144716	26.858747085684332	28.19705602383876	23.255894160332197
50-51	21.36320995488846	26.84502048643205	28.649344020307087	23.142425538372397
52-53	20.96582835542925	26.737414984755887	29.27490446562832	23.021852194186543
54-55	20.755583759846594	26.973250375929474	28.947190530715854	23.323975333508077
56-57	20.994799067418985	26.747968601266436	28.648792197221574	23.60844013409301
58-59	20.631492543490555	26.600011036461712	29.27145557134383	23.497040848703907
60-61	20.917543835446356	27.18494350711162	28.192227571840466	23.705285085601556
62-63	20.344406583249413	27.48106557037814	28.242443472622675	23.932084373749777
64-65	20.28653413715563	27.478168499179162	28.102487342557975	24.132810021107236
66-67	20.328127802226607	27.277856719135844	27.921420392622124	24.47259508601542
68-69	20.514161159932126	27.61915929752921	27.5343164981307	24.332363044407963
70-71	20.964655731372524	27.325727371804597	27.187495688882144	24.522121207940735
72-73	20.790279636348586	26.974078110557752	27.312483617752147	24.92315863534151
74-75	20.800281429773616	26.83253548912219	27.32538248237615	25.04180059872805
76	20.931201456812946	26.903030888297213	26.983735014554334	25.18203264033551
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	1.0
9	0.5
10	1.5
11	5.0
12	7.0
13	14.0
14	29.0
15	64.5
16	182.0
17	272.0
18	375.5
19	666.5
20	1109.5
21	1365.0
22	1763.0
23	2742.0
24	3892.5
25	4462.0
26	5413.5
27	7147.0
28	9212.0
29	10495.0
30	11677.5
31	14188.5
32	17174.5
33	18832.0
34	20408.5
35	23746.0
36	27421.0
37	29335.0
38	30715.0
39	34021.5
40	37563.0
41	39303.5
42	39429.0
43	39329.0
44	38877.0
45	37847.0
46	37169.0
47	36281.0
48	33766.5
49	31120.0
50	30100.0
51	28577.0
52	25211.5
53	22619.0
54	21869.0
55	20703.5
56	18628.5
57	16354.0
58	14989.0
59	13546.5
60	11130.5
61	9308.0
62	8459.0
63	7658.5
64	6378.5
65	5359.0
66	4630.0
67	4441.0
68	4211.5
69	3718.0
70	3303.5
71	3153.0
72	3063.0
73	2761.5
74	2413.0
75	2276.0
76	2079.5
77	1811.0
78	1505.0
79	1271.0
80	1135.5
81	894.5
82	669.0
83	549.0
84	455.5
85	283.0
86	178.5
87	153.0
88	111.0
89	54.5
90	30.0
91	16.5
92	13.0
93	8.5
94	3.5
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	724870.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.78251087720556
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.47277366327118	34.771979136145596
2	22.54616296028164	24.702708352217563
3	8.0316153849577	13.1997617156393
4	2.665839338934986	5.841654903283533
5	0.9669863445455442	2.6486969969087757
6	0.4307655389425792	1.4159050693588293
7	0.26968110261312045	1.034166555609604
8	0.21657825868854727	0.9491760649897263
9	0.165180908070693	0.8144122403780487
>10	1.1817125450873691	12.086851013856474
>50	0.049677947194515695	1.757906658482038
>100	0.002773840128180638	0.24686255944371815
>500	0.0	0.0
>1k	2.521672839940636E-4	0.5299187336868273
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGT	3836	0.5291983390125126	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.7591154275939135E-4	0.0
2	0.0	0.0	0.0	2.7591154275939135E-4	0.0
3	0.0	0.0	0.0	2.7591154275939135E-4	0.0
4	0.0	0.0	0.0	0.0013795577137969567	0.0
5	0.0	0.0	0.0	0.0013795577137969567	0.0
6	0.0	0.0	0.0	0.0013795577137969567	0.0
7	0.0	0.0	0.0	0.0013795577137969567	0.0
8	0.0	0.0	0.0	0.0015175134851766524	0.0
9	0.0	0.0	0.0	0.0017934250279360436	0.0
10	0.0	0.0	0.0	0.002069336570695435	0.0
11	1.3795577137969568E-4	0.0	0.0	0.002483203884834522	0.0
12	1.3795577137969568E-4	0.0	0.0	0.0031729827417330005	0.0
13	1.3795577137969568E-4	0.0	0.0	0.0034488942844923915	0.0
14	1.3795577137969568E-4	0.0	0.0	0.0035868500558720873	0.0
15	1.3795577137969568E-4	0.0	0.0	0.004276628912770566	0.0
16	1.3795577137969568E-4	0.0	0.0	0.004276628912770566	0.0
17	1.3795577137969568E-4	0.0	0.0	0.004690496226909653	0.0
18	1.3795577137969568E-4	0.0	0.0	0.004966407769669044	0.0
19	1.3795577137969568E-4	0.0	0.0	0.005380275083808131	0.0
20	1.3795577137969568E-4	0.0	0.0	0.005932098169326914	0.0
21	1.3795577137969568E-4	0.0	0.0	0.0060700539407066096	0.0
22	1.3795577137969568E-4	0.0	0.0	0.006897788568984783	0.0
23	1.3795577137969568E-4	0.0	0.0	0.007587567425883262	0.0
24	1.3795577137969568E-4	0.0	0.0	0.007863478968642652	0.0
25	1.3795577137969568E-4	0.0	0.0	0.008691213596920827	0.0
26	1.3795577137969568E-4	0.0	0.0	0.009794859767958393	0.0
27	1.3795577137969568E-4	0.0	0.0	0.014485355994868046	0.0
28	1.3795577137969568E-4	0.0	0.0	0.022072923420751307	0.0
29	1.3795577137969568E-4	0.0	0.0	0.035178721701822395	0.0
30	1.3795577137969568E-4	0.0	0.0	0.049526121925310744	0.0
31	1.3795577137969568E-4	0.0	0.0	0.06483921254845697	0.0
32	1.3795577137969568E-4	0.0	0.0	0.08056617048574227	0.0
33	1.3795577137969568E-4	0.0	0.0	0.09946611116476058	0.0
34	1.3795577137969568E-4	0.0	0.0	0.1335411866955454	0.0
35	1.3795577137969568E-4	0.0	0.0	0.17258266999599928	0.0
36	1.3795577137969568E-4	0.0	0.0	0.23962917488653138	0.0
37	1.3795577137969568E-4	0.0	0.0	0.3695835115262047	0.0
38	1.3795577137969568E-4	0.0	0.0	0.5529267316898202	0.0
39	1.3795577137969568E-4	0.0	0.0	0.786485852635645	0.0
40	1.3795577137969568E-4	0.0	0.0	1.0550857395119124	0.0
41	1.3795577137969568E-4	0.0	0.0	1.3287899899292286	0.0
42	1.3795577137969568E-4	0.0	0.0	1.6139445693710597	0.0
43	1.3795577137969568E-4	0.0	0.0	1.9044794238966987	0.0
44	1.3795577137969568E-4	0.0	0.0	2.2078441651606493	0.0
45	1.3795577137969568E-4	0.0	0.0	2.5741167381737413	0.0
46	1.3795577137969568E-4	0.0	0.0	2.9861906272848926	0.0
47	1.3795577137969568E-4	0.0	0.0	3.4295804764992344	0.0
48	1.3795577137969568E-4	0.0	0.0	3.9249796515237216	0.0
49	1.3795577137969568E-4	0.0	0.0	4.44272766151172	0.0
50	1.3795577137969568E-4	0.0	0.0	4.96820119469698	0.0
51	1.3795577137969568E-4	0.0	0.0	5.44428656172831	0.0
52	1.3795577137969568E-4	0.0	0.0	5.891125305227145	0.0
53	1.3795577137969568E-4	0.0	0.0	6.31451156759143	0.0
54	1.3795577137969568E-4	0.0	0.0	6.748382468580573	0.0
55	1.3795577137969568E-4	0.0	0.0	7.188875246595941	0.0
56	1.3795577137969568E-4	0.0	0.0	7.6532343730599965	0.0
57	1.3795577137969568E-4	0.0	0.0	8.104349745471602	0.0
58	1.3795577137969568E-4	0.0	0.0	8.557258542911143	0.0
59	1.3795577137969568E-4	0.0	0.0	9.06203871038945	0.0
60	1.3795577137969568E-4	0.0	0.0	9.575923958778816	0.0
61	1.3795577137969568E-4	0.0	0.0	10.085670534026791	0.0
62	1.3795577137969568E-4	0.0	0.0	10.55334059900396	0.0
63	1.3795577137969568E-4	0.0	0.0	10.985969898050685	0.0
64	1.3795577137969568E-4	0.0	0.0	11.425496985666395	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTCCG	20	0.0069510066	52.5	34
ACGTAGC	20	0.0069510066	52.5	7
TATGCCG	560	0.0	49.375	43
TCGTATG	565	0.0	48.938053	40
CTCGTAT	540	0.0	48.61111	39
TCTCGTA	480	0.0	47.395836	38
CGTATGC	585	0.0	47.264957	41
CCGTCTT	595	0.0	47.058823	47
CGTCTTC	615	0.0	46.097565	48
GCCGTCT	620	0.0	45.725807	46
ATGCCGT	645	0.0	43.953487	44
GTATGCC	635	0.0	43.543304	42
ATCTCGT	525	0.0	43.333332	37
TATCTCG	525	0.0	43.333332	36
TGCCGTC	685	0.0	41.386864	45
CGGTCAT	805	0.0	34.782604	28
GTCTTCT	855	0.0	32.748535	49
GCGAAGT	45	0.004307423	31.111109	32
TCGTGAG	45	0.004307423	31.111109	26
GGTCATT	905	0.0	30.165745	29
>>END_MODULE