FastQCFastQC Report
Wed 25 May 2016
SRR1781151_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781151_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93690
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCGGA150.002218511270.045
TACCGCT150.002218511270.059
TACCACG200.00693221452.540
ACCGCTA200.00693221452.560
TTAGAGT200.00693221452.54
CAACCTA200.00693221452.568
GAGACGC200.00693221452.565
TAGATGC200.00693221452.54
TGTAACG200.00693221452.536
CTTGAGT350.001249872640.012
GTATAGA400.00240814235.01
ACGTACA400.00240814235.043
CTGGGCG400.00240814235.028
TCTACAC552.972655E-431.818183
GCCGAGA450.00428847831.11110948
GGTATTG450.00428847831.1111091
CGTACAT450.00428847831.11110944
GCCAACA450.00428847831.11110943
CTTCTAG604.9498305E-429.1666661
GTCTATT500.007177128527.9999982