##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781151_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	93690
Sequences flagged as poor quality	0
Sequence length	76
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.193670615860817	34.0	32.0	34.0	21.0	34.0
2	31.277959227238767	34.0	33.0	34.0	23.0	34.0
3	31.202412210481373	34.0	33.0	34.0	23.0	34.0
4	31.332415412530686	34.0	33.0	34.0	23.0	34.0
5	31.354669655246024	34.0	33.0	34.0	23.0	34.0
6	34.06167146974063	37.0	34.0	38.0	24.0	38.0
7	34.052961895613194	37.0	34.0	38.0	24.0	38.0
8	33.844391076955915	37.0	34.0	38.0	23.0	38.0
9	34.12664105027218	37.0	34.0	38.0	24.0	38.0
10-11	33.93471555128616	37.0	34.0	38.0	23.0	38.0
12-13	33.90786103105988	37.0	34.0	38.0	23.0	38.0
14-15	33.785494716618636	37.0	34.0	38.0	22.5	38.0
16-17	33.73594834027111	37.0	34.0	38.0	22.5	38.0
18-19	33.761890276443594	37.0	34.0	38.0	22.5	38.0
20-21	33.6897427687053	37.0	34.0	38.0	22.5	38.0
22-23	33.75794108229267	37.0	34.0	38.0	22.5	38.0
24-25	33.73043547870637	37.0	34.0	38.0	22.5	38.0
26-27	33.644401750453625	37.0	34.0	38.0	22.0	38.0
28-29	33.75102999252855	37.0	34.0	38.0	22.5	38.0
30-31	33.79894866047604	37.0	34.0	38.0	23.0	38.0
32-33	33.75446152204077	37.0	34.0	38.0	22.5	38.0
34-35	33.7464083680222	37.0	34.0	38.0	22.5	38.0
36-37	33.625130750346884	37.0	34.0	38.0	22.0	38.0
38-39	33.649583733589495	37.0	34.0	38.0	22.0	38.0
40-41	33.63619383071833	37.0	34.0	38.0	22.0	38.0
42-43	33.70769559184545	37.0	34.0	38.0	22.0	38.0
44-45	33.73317323086775	37.0	34.0	38.0	22.5	38.0
46-47	33.7212562706799	37.0	34.0	38.0	22.5	38.0
48-49	33.69614686732842	37.0	34.0	38.0	22.5	38.0
50-51	33.56326182089871	37.0	34.0	38.0	22.0	38.0
52-53	33.63453943857402	37.0	34.0	38.0	22.0	38.0
54-55	33.62768171629843	37.0	34.0	38.0	22.0	38.0
56-57	33.58892624613085	37.0	34.0	38.0	22.0	38.0
58-59	33.53365353826449	37.0	34.0	38.0	22.0	38.0
60-61	33.56571138862205	37.0	34.0	38.0	22.0	38.0
62-63	33.520546483082505	37.0	34.0	38.0	22.0	38.0
64-65	33.53012594727292	37.0	34.0	38.0	22.0	38.0
66-67	33.41333653538264	37.0	34.0	38.0	21.0	38.0
68-69	33.153687693457144	37.0	33.5	38.0	21.0	38.0
70-71	33.30987298537731	37.0	34.0	38.0	21.0	38.0
72-73	33.309931689614686	37.0	33.5	38.0	21.0	38.0
74-75	33.32652364179742	37.0	33.0	38.0	21.0	38.0
76	32.05267371117515	36.0	31.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	5.0
13	15.0
14	88.0
15	229.0
16	467.0
17	867.0
18	1057.0
19	1038.0
20	931.0
21	906.0
22	903.0
23	921.0
24	1111.0
25	1283.0
26	1452.0
27	1872.0
28	2155.0
29	2554.0
30	3119.0
31	3679.0
32	4451.0
33	5544.0
34	7184.0
35	9398.0
36	14203.0
37	28258.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.63688760806916	17.428754402817802	9.021240260433345	25.91311772867969
2	23.816842779378803	19.89646707225958	30.535809584800937	25.75088056356068
3	20.612658768278365	21.035329277404205	28.808837656099907	29.54317429821753
4	14.955704984523429	16.0540078983883	35.04963176432917	33.940655352759094
5	15.045362365247092	31.70135553420856	35.32821005443484	17.92507204610951
6	30.559291279752376	35.279111964990925	18.221795282314016	15.939801472942683
7	29.607215284448714	28.257017824741165	21.622371651190093	20.513395239620024
8	26.28882484790266	33.01206105240687	19.711815561959654	20.987298537730815
9	24.985590778097983	15.979293414451917	20.38424591738713	38.65086989006297
10-11	26.033194577863167	23.84192549898602	26.52470914718753	23.600170775963285
12-13	25.976625040025613	21.844380403458214	25.973956665599317	26.205037890916856
14-15	24.133311986337922	22.899455651617036	23.380830398121464	29.586401963923574
16-17	24.332906393425123	26.088696765930198	24.279005230013876	25.299391610630806
18-19	23.841391824100757	25.571565802113355	25.372505069911412	25.21453730387448
20-21	24.113566015583306	25.32447433023802	25.49151456932437	25.07044508485431
22-23	24.322766570605186	24.883658875013342	25.797843953463552	24.99573060091792
24-25	24.26032660902978	25.26150069377735	25.391717365780764	25.086455331412104
26-27	23.626320845341017	25.392784715551286	25.85868289038318	25.122211548724515
28-29	23.99082079197353	25.303661009712886	26.198100117408472	24.507418080905115
30-31	24.03725050699114	25.024549044721955	25.738606041199702	25.1995944070872
32-33	23.694097555769027	25.400789838830185	25.84267264382538	25.062439961575407
34-35	24.143451809157863	24.718753335468033	26.092432490127017	25.045362365247094
36-37	24.14665385846942	24.78653004589604	25.826662397267587	25.240153698366957
38-39	23.50997972035436	24.749172803927845	26.7061586081759	25.03468886754189
40-41	23.88355214003629	24.486071085494718	26.76112712135767	24.869249653111325
42-43	23.18657273988686	24.5741274415626	26.435051766463868	25.80424805208667
44-45	22.98857935745544	24.67552566976198	27.02636353933184	25.309531433450744
46-47	23.056356067883446	24.74490340484577	27.221688547336964	24.977051979933822
48-49	22.60646813960935	24.73636460668161	27.222755897107483	25.43441135660156
50-51	22.51147401003309	24.73156153271427	27.260113139075674	25.496851318176965
52-53	22.325755149962642	24.724090084320633	27.715337816202368	25.234816949514354
54-55	22.138435265236417	25.154232041840114	27.357775643078234	25.349557049845235
56-57	21.930835734870318	25.337816202369517	27.1346995410396	25.596648521720567
58-59	22.364179741701356	24.713950261500695	26.951649055395453	25.9702209414025
60-61	21.997545095527805	24.61201835841605	27.248905966485214	26.141530579570926
62-63	21.538584694204292	24.61628775749813	27.42715337816202	26.417974170135555
64-65	22.172056783007793	24.708613512648096	26.864126374212834	26.255203330131284
66-67	22.346568470487778	24.763582025829862	26.238659408688225	26.65119009499413
68-69	22.139502615006936	24.813213790159036	26.121784608816306	26.925498986017722
70-71	22.546162877574982	24.90073647134166	26.47881310705518	26.07428754402818
72-73	22.40900843206319	24.655779699007365	26.238659408688225	26.696552460241218
74-75	21.91269078877148	25.04216031593553	26.445725264169067	26.59942363112392
76	22.39193083573487	24.703810438680758	26.238659408688225	26.66559931689615
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	1.0
7	0.5
8	0.0
9	0.0
10	0.0
11	0.5
12	1.0
13	1.5
14	3.0
15	10.0
16	22.0
17	28.0
18	38.5
19	63.5
20	93.0
21	108.0
22	112.5
23	166.0
24	270.5
25	326.0
26	379.0
27	490.0
28	646.5
29	745.0
30	910.0
31	1124.5
32	1428.5
33	1683.0
34	1822.5
35	2164.0
36	2519.5
37	2673.0
38	3087.5
39	3695.0
40	4155.5
41	4692.5
42	4962.0
43	4985.0
44	5016.5
45	4990.0
46	4955.0
47	4935.0
48	4795.5
49	4346.0
50	4016.0
51	3764.0
52	3218.0
53	2838.5
54	2753.0
55	2590.0
56	2262.0
57	1957.5
58	1818.0
59	1728.0
60	1532.5
61	1401.5
62	1376.0
63	1308.5
64	1237.0
65	1210.0
66	1182.0
67	1177.0
68	1160.5
69	1151.0
70	1162.0
71	1166.0
72	1126.5
73	1081.5
74	1024.5
75	973.0
76	897.5
77	765.0
78	643.0
79	578.0
80	494.0
81	350.0
82	242.5
83	195.0
84	154.5
85	102.5
86	63.5
87	36.0
88	29.5
89	19.0
90	11.0
91	6.5
92	6.0
93	4.0
94	2.0
95	1.0
96	0.0
97	0.5
98	0.5
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	93690.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.43046216245064
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.88941239819614	46.86839577329491
2	14.73379551726459	18.691429181342727
3	5.46207175068991	10.393852065321806
4	2.2834354176482465	5.793574554381471
5	1.108904893316282	3.516917493862739
6	0.649525476206502	2.471982068523855
7	0.4644275425725247	2.062119756644252
8	0.32308002961566934	1.6394492475184117
9	0.2540889816248233	1.4505283381364074
>10	0.831257992865316	7.1117515209734234
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0010673497705197994	0.0
2	0.0	0.0	0.0	0.002134699541039599	0.0
3	0.0	0.0	0.0	0.002134699541039599	0.0
4	0.0	0.0	0.0	0.002134699541039599	0.0
5	0.0	0.0	0.0	0.002134699541039599	0.0
6	0.0	0.0	0.0	0.004269399082079198	0.0
7	0.0	0.0	0.0	0.004269399082079198	0.0
8	0.0	0.0	0.0	0.004269399082079198	0.0
9	0.0	0.0	0.0	0.005336748852598996	0.0
10	0.0	0.0	0.0	0.005336748852598996	0.0
11	0.0	0.0	0.0	0.006404098623118796	0.0
12	0.0	0.0	0.0	0.007471448393638595	0.0
13	0.0	0.0	0.0	0.007471448393638595	0.0
14	0.0	0.0	0.0	0.007471448393638595	0.0
15	0.0	0.0	0.0	0.007471448393638595	0.0
16	0.0	0.0	0.0	0.007471448393638595	0.0
17	0.0	0.0	0.0	0.007471448393638595	0.0
18	0.0	0.0	0.0	0.008538798164158395	0.0
19	0.0	0.0	0.0	0.011740847475717792	0.0
20	0.0	0.0	0.0	0.011740847475717792	0.0
21	0.0	0.0	0.0	0.011740847475717792	0.0
22	0.0	0.0	0.0	0.01814494609883659	0.0
23	0.0	0.0	0.0	0.02027964563987619	0.0
24	0.0	0.0	0.0	0.02027964563987619	0.0
25	0.0	0.0	0.0	0.022414345180915785	0.0
26	0.0	0.0	0.0	0.023481694951435584	0.0
27	0.0	0.0	0.0	0.039491941509232575	0.0
28	0.0	0.0	0.0	0.060838936919628564	0.0
29	0.0	0.0	0.0	0.08859003095314334	0.0
30	0.0	0.0	0.0	0.11527377521613832	0.0
31	0.0	0.0	0.0	0.14195751947913332	0.0
32	0.0	0.0	0.0	0.1750453623652471	0.0
33	0.0	0.0	0.0	0.23161490020279646	0.0
34	0.0	0.0	0.0	0.3170028818443804	0.0
35	0.0	0.0	0.0	0.4450848543067563	0.0
36	0.0	0.0	0.0	0.6404098623118796	0.0
37	0.0	0.0	0.0	0.9179208026470275	0.0
38	0.0	0.0	0.0	1.1890276443590564	0.0
39	0.0	0.0	0.0	1.5348489700074714	0.0
40	0.0	0.0	0.0	1.8603906500160103	0.0
41	0.0	0.0	0.0	2.186999679795069	0.0
42	0.0	0.0	0.0	2.584053794428434	0.0
43	0.0	0.0	0.0	3.016330451488953	0.0
44	0.0	0.0	0.0	3.5542747358309317	0.0
45	0.0	0.0	0.0	4.171202903191376	0.0
46	0.0	0.0	0.0	4.725157434091152	0.0
47	0.0	0.0	0.0	5.290852812466645	0.0
48	0.0	0.0	0.0	5.859750240153699	0.0
49	0.0	0.0	0.0	6.371010780232682	0.0
50	0.0	0.0	0.0	6.843846728572953	0.0
51	0.0	0.0	0.0	7.3209520759953035	0.0
52	0.0	0.0	0.0	7.800192122958694	0.0
53	0.0	0.0	0.0	8.308250613726118	0.0
54	0.0	0.0	0.0	8.83445405059238	0.0
55	0.0	0.0	0.0	9.355320738606041	0.0
56	0.0	0.0	0.0	9.923150816522574	0.0
57	0.0	0.0	0.0	10.457893051552993	0.0
58	0.0	0.0	0.0	10.979827089337176	0.0
59	0.0	0.0	0.0	11.501761127121357	0.0
60	0.0	0.0	0.0	11.986337922937347	0.0
61	0.0	0.0	0.0	12.403671683210588	0.0
62	0.0	0.0	0.0	12.848756537517344	0.0
63	0.0	0.0	0.0	13.323727185398655	0.0
64	0.0	0.0	0.0	13.825381577542961	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCGGA	15	0.0022185112	70.0	45
TACCGCT	15	0.0022185112	70.0	59
TACCACG	20	0.006932214	52.5	40
ACCGCTA	20	0.006932214	52.5	60
TTAGAGT	20	0.006932214	52.5	4
CAACCTA	20	0.006932214	52.5	68
GAGACGC	20	0.006932214	52.5	65
TAGATGC	20	0.006932214	52.5	4
TGTAACG	20	0.006932214	52.5	36
CTTGAGT	35	0.0012498726	40.0	12
GTATAGA	40	0.002408142	35.0	1
ACGTACA	40	0.002408142	35.0	43
CTGGGCG	40	0.002408142	35.0	28
TCTACAC	55	2.972655E-4	31.81818	3
GCCGAGA	45	0.004288478	31.111109	48
GGTATTG	45	0.004288478	31.111109	1
CGTACAT	45	0.004288478	31.111109	44
GCCAACA	45	0.004288478	31.111109	43
CTTCTAG	60	4.9498305E-4	29.166666	1
GTCTATT	50	0.0071771285	27.999998	2
>>END_MODULE