FastQCFastQC Report
Wed 25 May 2016
SRR1781151_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781151_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87678
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT25032.8547640229019824No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG2850.32505303496886334No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCC2610.29768014781359065No Hit
CTTATACACATCTCCGAGCCCACGAGACTACACCTCACCTCGTATGCCGT1640.1870480622276968No Hit
CTTATACACATCTCCGAGCCCACGAGACTACACCTCAACTCGTATGCCGT920.1049294007618787No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACTA150.002218035370.01
AACTCGT452.6651833E-854.44444737
CTTCGCA200.00693073952.527
TGTGGCC200.00693073952.521
ACCCTGC200.00693073952.56
GATATTC200.00693073952.536
TGGTCTA200.00693073952.515
CGTATGC3900.050.2564141
TATGCCG3850.050.00000443
TCGTATG3850.050.00000440
CTCGTAT3900.049.35897439
ACTCGTA506.113805E-848.99999638
TTGAAAA3650.048.9041158
CCGTCTT3800.048.8157947
CTTGAAA3700.048.24324457
ATCTCGT3200.048.12537
TCTCGTA3200.048.12538
CTGCTTG3800.047.89473354
CGTCTTC3950.047.84810348
GTATGCC4100.047.8048842
Produced by FastQC (version 0.11.5)