##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781151_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	87678
Sequences flagged as poor quality	0
Sequence length	76
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.22979538766852	34.0	34.0	34.0	32.0	34.0
2	33.26524327653459	34.0	34.0	34.0	33.0	34.0
3	33.35076073815552	34.0	34.0	34.0	33.0	34.0
4	33.41679782841762	34.0	34.0	34.0	34.0	34.0
5	33.40858596227104	34.0	34.0	34.0	34.0	34.0
6	36.812130751157646	38.0	37.0	38.0	34.0	38.0
7	36.70648281210794	38.0	37.0	38.0	34.0	38.0
8	36.46513378498597	38.0	37.0	38.0	34.0	38.0
9	36.48444307580009	38.0	37.0	38.0	34.0	38.0
10-11	36.385108008850565	38.0	37.0	38.0	34.0	38.0
12-13	36.340290608818634	38.0	37.0	38.0	34.0	38.0
14-15	36.33608773010333	38.0	37.0	38.0	34.0	38.0
16-17	36.269662857273204	38.0	37.0	38.0	34.0	38.0
18-19	36.2538949337348	38.0	37.0	38.0	34.0	38.0
20-21	36.20175528638883	38.0	37.0	38.0	33.5	38.0
22-23	36.27639772805037	38.0	37.0	38.0	34.0	38.0
24-25	36.23400967175346	38.0	37.0	38.0	34.0	38.0
26-27	36.02880996373092	38.0	36.5	38.0	32.5	38.0
28-29	36.00373525855973	38.0	37.0	38.0	32.0	38.0
30-31	36.03910330983827	38.0	37.0	38.0	32.0	38.0
32-33	35.852425922124134	38.0	36.0	38.0	31.0	38.0
34-35	35.90403521978148	38.0	36.0	38.0	31.5	38.0
36-37	35.93314172312324	38.0	36.5	38.0	32.0	38.0
38-39	35.671171787677636	38.0	36.0	38.0	31.0	38.0
40-41	35.8372909966012	38.0	36.0	38.0	31.5	38.0
42-43	35.899410342389196	38.0	36.5	38.0	31.5	38.0
44-45	35.9015488491982	38.0	36.0	38.0	32.0	38.0
46-47	35.915126941764186	38.0	37.0	38.0	32.0	38.0
48-49	35.89480827573622	38.0	36.0	38.0	32.0	38.0
50-51	35.77018750427702	38.0	36.0	38.0	31.0	38.0
52-53	35.802208079563854	38.0	36.0	38.0	31.0	38.0
54-55	35.80857227582746	38.0	36.0	38.0	31.0	38.0
56-57	35.74065329957344	38.0	36.0	38.0	31.0	38.0
58-59	35.69062934829718	38.0	36.0	38.0	31.0	38.0
60-61	35.6850065010607	38.0	36.0	38.0	31.0	38.0
62-63	35.65536394534547	38.0	36.0	38.0	31.0	38.0
64-65	35.63254180067976	38.0	36.0	38.0	31.0	38.0
66-67	35.55885170738384	38.0	36.0	38.0	31.0	38.0
68-69	35.40474235269965	38.0	36.0	38.0	31.0	38.0
70-71	35.14671867515226	38.0	36.0	38.0	28.0	38.0
72-73	34.92129724674376	38.0	36.0	38.0	27.0	38.0
74-75	34.90497046009261	38.0	36.0	38.0	27.0	38.0
76	34.04737790551792	37.0	34.0	38.0	24.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	3.0
21	3.0
22	16.0
23	29.0
24	95.0
25	149.0
26	298.0
27	507.0
28	818.0
29	1287.0
30	1987.0
31	2829.0
32	3859.0
33	5547.0
34	7441.0
35	10253.0
36	15101.0
37	37456.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.67597344829946	17.33844293893565	8.974885376035036	29.010698236729855
2	23.17000843997354	19.5031820981318	32.3433472478843	24.98346221401036
3	20.56502201236342	20.753210611555918	30.494536827938596	28.187230548142068
4	14.775656379023244	19.174707452268528	34.11688222815302	31.932753940555216
5	14.406122402427062	32.135769520290154	36.835922352243436	16.622185725039348
6	30.332580578936565	38.26387463217683	17.072697826136547	14.330846962750062
7	29.1030817308789	27.768653482059353	20.089418098040557	23.03884668902119
8	25.65181687538493	36.087730103332646	17.923538401879604	20.336914619402815
9	24.294577887269327	15.578594402244578	19.22489107871986	40.90193663176623
10-11	25.53091995711581	24.853441000022812	26.89329136157303	22.72234768128835
12-13	25.224115513583794	21.52820547913958	26.19186112821917	27.055817879057457
14-15	23.410661739546978	22.254727525719108	22.12356577476676	32.21104495996715
16-17	25.814913661351763	26.81744565341363	22.773671844704488	24.593968840530124
18-19	25.636989894842493	24.928146171217406	24.004311229726955	25.430552704213145
20-21	23.43404274732544	24.87283012842446	23.671844704486873	28.021282419763228
22-23	23.756244439882295	26.082369579598076	24.01742740482219	26.14395857569744
24-25	25.837724400647826	26.25687173521294	23.76764980953033	24.13775405460891
26-27	24.996008120623188	26.694267661214898	24.317388626565386	23.99233559159652
28-29	23.27208649832341	24.761627774356167	26.47129268459591	25.494993042724513
30-31	23.44430758000867	26.258582540660143	24.65669837359429	25.6404115057369
32-33	23.06108715983485	27.21150117475307	23.699217591642142	26.028194073769935
34-35	23.734003969068638	24.00317069276215	26.37662811651726	25.88619722165195
36-37	23.59257738543306	25.66721412440977	24.600812062318937	26.139396427838225
38-39	22.821574397226215	25.028513424120074	26.538014097036882	25.611898081616825
40-41	22.966992860238598	23.62622322589475	27.42877346654805	25.9780104473186
42-43	25.155683295695614	23.615388124729122	26.966855995802824	24.26207258377244
44-45	22.539291498437464	25.930107894796873	27.569059513218825	23.961541093546842
46-47	24.55576085220922	23.93758981728598	25.897602591299986	25.609046739204818
48-49	23.949565455416412	23.616528661693927	25.679760031022607	26.75414585186706
50-51	22.060836241702596	23.841214443760123	27.754967038481716	26.342982276055565
52-53	21.593786354615755	23.73913638541025	30.29038071123885	24.376696548735143
54-55	21.175209288533043	23.854330618855357	28.585277948858323	26.385182143753276
56-57	23.40666986017017	24.12235680558407	25.951207828645728	26.519765505600034
58-59	21.568694541390087	23.988913980702115	29.105362804808504	25.337028673099294
60-61	23.167727366043934	25.6392709687721	26.161636898651885	25.031364766532082
62-63	20.817650950067293	28.227719610392572	25.610187276169622	25.344442163370516
64-65	20.928283035653187	28.560756404115057	24.959510937749492	25.551449622482263
66-67	21.194598416934692	29.05803052076918	24.2843130545861	25.463058007710032
68-69	21.030361094003055	29.081981797030043	24.34133990282625	25.54631720614065
70-71	21.240790164009216	27.935742147402998	24.803257373571476	26.020210315016314
72-73	21.086247405278407	26.51805470015283	25.197312894910922	27.198384999657836
74-75	20.899199343050707	26.111453272200553	25.273728871552727	27.71561851319601
76	21.532197358516388	26.00196172357946	25.01995939688405	27.445881521020098
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	1.0
14	2.5
15	5.0
16	7.5
17	8.0
18	25.5
19	43.0
20	56.0
21	69.0
22	109.5
23	176.0
24	256.0
25	310.0
26	375.0
27	468.5
28	600.5
29	704.0
30	813.0
31	972.0
32	1275.5
33	1529.0
34	1619.0
35	1924.5
36	2304.0
37	2468.0
38	2658.5
39	3198.0
40	3993.5
41	4804.0
42	5168.0
43	5310.0
44	5414.5
45	5153.0
46	4929.0
47	4843.0
48	4379.5
49	3819.5
50	3637.0
51	3311.5
52	2778.5
53	2373.5
54	2176.0
55	2178.0
56	1991.0
57	1699.5
58	1597.0
59	1519.0
60	1391.5
61	1291.5
62	1241.0
63	1161.0
64	1099.5
65	1109.5
66	1123.0
67	1145.0
68	1120.5
69	1124.5
70	1151.0
71	1149.0
72	1089.0
73	1002.0
74	941.5
75	908.0
76	855.0
77	732.0
78	609.5
79	557.0
80	469.5
81	331.0
82	253.5
83	227.0
84	169.5
85	87.0
86	49.5
87	37.0
88	28.0
89	15.0
90	7.5
91	3.5
92	3.0
93	2.5
94	1.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	87678.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.60751841967198
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.99727671162929	34.30735190127512
2	20.3246670354453	21.791099249526678
3	7.576273350070211	12.184356394990761
4	3.070082124164929	6.583179360843085
5	1.5318497085230416	4.105933073290905
6	0.9403855155099783	3.0247040306576336
7	0.6552912642015234	2.4589976961153313
8	0.49785115526998847	2.135085198111271
9	0.3191353559423003	1.5397249024840896
>10	1.072294795966129	7.955245329501129
>50	0.006382707118846007	0.2497775952918634
>100	0.006382707118846007	0.8097812450101507
>500	0.0	0.0
>1k	0.0021275690396153355	2.8547640229019824
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT	2503	2.8547640229019824	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG	285	0.32505303496886334	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCC	261	0.29768014781359065	No Hit
CTTATACACATCTCCGAGCCCACGAGACTACACCTCACCTCGTATGCCGT	164	0.1870480622276968	No Hit
CTTATACACATCTCCGAGCCCACGAGACTACACCTCAACTCGTATGCCGT	92	0.1049294007618787	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0022810739296060586	0.0
2	0.0	0.0	0.0	0.003421610894409088	0.0
3	0.0	0.0	0.0	0.003421610894409088	0.0
4	0.0	0.0	0.0	0.003421610894409088	0.0
5	0.0	0.0	0.0	0.003421610894409088	0.0
6	0.0	0.0	0.0	0.0057026848240151464	0.0
7	0.0	0.0	0.0	0.0057026848240151464	0.0
8	0.0	0.0	0.0	0.0057026848240151464	0.0
9	0.0	0.0	0.0	0.006843221788818176	0.0
10	0.0	0.0	0.0	0.006843221788818176	0.0
11	0.0	0.0	0.0	0.006843221788818176	0.0
12	0.0	0.0	0.0	0.007983758753621204	0.0
13	0.0	0.0	0.0	0.007983758753621204	0.0
14	0.0	0.0	0.0	0.007983758753621204	0.0
15	0.0	0.0	0.0	0.007983758753621204	0.0
16	0.0	0.0	0.0	0.007983758753621204	0.0
17	0.0	0.0	0.0	0.009124295718424234	0.0
18	0.0	0.0	0.0	0.010264832683227263	0.0
19	0.0	0.0	0.0	0.01596751750724241	0.0
20	0.0	0.0	0.0	0.01596751750724241	0.0
21	0.0	0.0	0.0	0.01710805447204544	0.0
22	0.0	0.0	0.0	0.01710805447204544	0.0
23	0.0	0.0	0.0	0.021670202331257556	0.0
24	0.0	0.0	0.0	0.021670202331257556	0.0
25	0.0	0.0	0.0	0.023951276260863616	0.0
26	0.0	0.0	0.0	0.025091813225666643	0.0
27	0.0	0.0	0.0	0.04334040466251511	0.0
28	0.0	0.0	0.0	0.06158899609936358	0.0
29	0.0	0.0	0.0	0.09124295718424234	0.0
30	0.0	0.0	0.0	0.12203745523392413	0.0
31	0.0	0.0	0.0	0.15397249024840895	0.0
32	0.0	0.0	0.0	0.1870480622276968	0.0
33	0.0	0.0	0.0	0.2497775952918634	0.0
34	0.0	0.0	0.0	0.33645840461689364	0.0
35	0.0	0.0	0.0	0.466479618604439	0.0
36	0.0	0.0	0.0	0.6672141244097721	0.0
37	0.0	0.0	0.0	0.9546294395401355	0.0
38	0.0	0.0	0.0	1.240904217705696	0.0
39	0.0	0.0	0.0	1.6115787312666803	0.0
40	0.0	0.0	0.0	1.9400533771299528	0.0
41	0.0	0.0	0.0	2.2810739296060585	0.0
42	0.0	0.0	0.0	2.6825429412167248	0.0
43	0.0	0.0	0.0	3.121649672665891	0.0
44	0.0	0.0	0.0	3.662264193982527	0.0
45	0.0	0.0	0.0	4.278154154976162	0.0
46	0.0	0.0	0.0	4.856406396131298	0.0
47	0.0	0.0	0.0	5.446064006934464	0.0
48	0.0	0.0	0.0	6.030018932913616	0.0
49	0.0	0.0	0.0	6.556947010652615	0.0
50	0.0	0.0	0.0	7.032550924975478	0.0
51	0.0	0.0	0.0	7.505873765368736	0.0
52	0.0	0.0	0.0	7.9917425123748265	0.0
53	0.0	0.0	0.0	8.5095462943954	0.0
54	0.0	0.0	0.0	9.051301352676841	0.0
55	0.0	0.0	0.0	9.57822943041584	0.0
56	0.0	0.0	0.0	10.154200597641369	0.0
57	0.0	0.0	0.0	10.76438787381099	0.0
58	0.0	0.0	0.0	11.298159173338808	0.0
59	0.0	0.0	0.0	11.851319601268278	0.0
60	0.0	0.0	0.0	12.370263920253656	0.0
61	0.0	0.0	0.0	12.802527429914004	0.0
62	0.0	0.0	0.0	13.266725974588837	0.0
63	0.0	0.0	0.0	13.7423298889117	0.0
64	0.0	0.0	0.0	14.24758776431944	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTACTA	15	0.0022180353	70.0	1
AACTCGT	45	2.6651833E-8	54.444447	37
CTTCGCA	20	0.006930739	52.5	27
TGTGGCC	20	0.006930739	52.5	21
ACCCTGC	20	0.006930739	52.5	6
GATATTC	20	0.006930739	52.5	36
TGGTCTA	20	0.006930739	52.5	15
CGTATGC	390	0.0	50.25641	41
TATGCCG	385	0.0	50.000004	43
TCGTATG	385	0.0	50.000004	40
CTCGTAT	390	0.0	49.358974	39
ACTCGTA	50	6.113805E-8	48.999996	38
TTGAAAA	365	0.0	48.90411	58
CCGTCTT	380	0.0	48.81579	47
CTTGAAA	370	0.0	48.243244	57
ATCTCGT	320	0.0	48.125	37
TCTCGTA	320	0.0	48.125	38
CTGCTTG	380	0.0	47.894733	54
CGTCTTC	395	0.0	47.848103	48
GTATGCC	410	0.0	47.80488	42
>>END_MODULE