FastQCFastQC Report
Wed 25 May 2016
SRR1781150_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781150_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161295
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGCT150.002221427670.045
GATCCAT200.00694125852.543
AGGACCG200.00694125852.55
ACTTAGA200.00694125852.527
ACGGGCT200.00694125852.540
CCCAACG200.00694125852.554
GGGAACG200.00694125852.568
GGAACGT200.00694125852.569
TAGATAA200.00694125852.516
GTCTTTC200.00694125852.58
CTCTCGC200.00694125852.544
CCTCTCG305.8642955E-446.66666443
GTTAAGC350.001252561540.021
GTTCTAC459.166753E-538.88888535
CGTCCAA459.166753E-538.88888540
CGAACGA400.002413291535.014
TGGTTCG400.002413291535.035
CTTGCGG400.002413291535.034
GTACGAA400.002413291535.01
TCTTGGA552.9821353E-431.81818448
Produced by FastQC (version 0.11.5)