##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781150_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	161295
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.181040949812456	34.0	32.0	34.0	21.0	34.0
2	31.397327877491552	34.0	33.0	34.0	23.0	34.0
3	31.45559998760036	34.0	33.0	34.0	23.0	34.0
4	31.48709507424285	34.0	33.0	34.0	23.0	34.0
5	31.565975386713784	34.0	33.0	34.0	23.0	34.0
6	34.477305558138816	37.0	34.0	38.0	26.0	38.0
7	34.421829566942556	37.0	34.0	38.0	25.0	38.0
8	34.19707988468335	37.0	34.0	38.0	24.0	38.0
9	34.4825258067516	37.0	34.0	38.0	26.0	38.0
10-11	34.335537369416286	37.0	34.0	38.0	24.5	38.0
12-13	34.32097709166434	37.0	34.0	38.0	24.5	38.0
14-15	34.20902073839859	37.0	34.0	38.0	24.0	38.0
16-17	34.16578319228742	37.0	34.0	38.0	24.0	38.0
18-19	34.23002572925385	37.0	34.0	38.0	24.0	38.0
20-21	34.15333705322546	37.0	34.0	38.0	24.0	38.0
22-23	34.22917945379584	37.0	34.0	38.0	24.5	38.0
24-25	34.2217644688304	37.0	34.0	38.0	24.0	38.0
26-27	34.13450819926222	37.0	34.0	38.0	24.0	38.0
28-29	34.22131188195542	37.0	34.0	38.0	24.0	38.0
30-31	34.28290089587402	37.0	34.0	38.0	24.0	38.0
32-33	34.24399082426609	37.0	34.0	38.0	24.0	38.0
34-35	34.28125794351964	37.0	34.0	38.0	24.0	38.0
36-37	34.12598654639015	37.0	34.0	38.0	24.0	38.0
38-39	34.132372361201526	37.0	34.0	38.0	24.0	38.0
40-41	34.12776899469915	37.0	34.0	38.0	24.0	38.0
42-43	34.19087696456803	37.0	34.0	38.0	24.0	38.0
44-45	34.19859884063362	37.0	34.0	38.0	24.0	38.0
46-47	34.195666325676555	37.0	34.0	38.0	24.0	38.0
48-49	34.164419231842274	37.0	34.0	38.0	24.0	38.0
50-51	34.00907343687033	37.0	34.0	38.0	24.0	38.0
52-53	34.09372268204223	37.0	34.0	38.0	24.0	38.0
54-55	34.062835177779846	37.0	34.0	38.0	24.0	38.0
56-57	34.04701323661614	37.0	34.0	38.0	24.0	38.0
58-59	34.04998295049444	37.0	34.0	38.0	24.0	38.0
60-61	34.001171766018786	37.0	34.0	38.0	24.0	38.0
62-63	33.95958337208221	37.0	34.0	38.0	23.5	38.0
64-65	33.99566012585635	37.0	34.0	38.0	24.0	38.0
66-67	33.90328590470877	37.0	34.0	38.0	23.0	38.0
68-69	33.67626398834434	37.0	34.0	38.0	22.5	38.0
70-71	33.8596918689358	37.0	34.0	38.0	23.5	38.0
72-73	33.838885272327104	37.0	34.0	38.0	23.0	38.0
74-75	33.87635078582721	37.0	34.0	38.0	23.5	38.0
76	32.520183514678074	37.0	31.0	38.0	21.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	19.0
14	66.0
15	191.0
16	389.0
17	640.0
18	923.0
19	1053.0
20	1142.0
21	1201.0
22	1391.0
23	1602.0
24	1878.0
25	2102.0
26	2592.0
27	3223.0
28	3777.0
29	4484.0
30	5369.0
31	6606.0
32	7901.0
33	9786.0
34	12524.0
35	16699.0
36	25185.0
37	50549.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.5879909482625	17.156762453888838	8.852103289004619	25.403143308844044
2	24.393812579435195	20.61936203850088	30.15344555007905	24.83337983198487
3	20.043398741436498	21.704950556433865	29.64754022133358	28.60411048079606
4	14.049412567035555	16.87219070646951	36.947828512973125	32.13056821352181
5	13.73756161071329	33.020862394990544	36.42146377755045	16.820112216745713
6	28.42679562292694	37.867881831426885	19.25912148547692	14.446201060169257
7	29.295390433677422	29.52416379924982	22.200936172850987	18.979509594221767
8	26.322576645277284	34.08227161412319	19.703028612170247	19.892123128429276
9	25.1005920828296	16.823212126848322	21.251743699432716	36.824452090889366
10-11	25.334325304566168	24.2896555999876	27.723116029635143	22.652903065811092
12-13	25.66229579342199	22.786819182243715	26.444403112309743	25.106481912024552
14-15	23.83427880591463	23.868997799063827	23.95734523698813	28.33937815803342
16-17	23.942465668495615	26.83189187513562	25.152670572553397	24.07297188381537
18-19	23.187637558510804	26.635667565640595	26.568399516414026	23.608295359434575
20-21	23.224836479742088	26.74385442822158	26.50578133234136	23.52552775969497
22-23	23.45174989925292	26.265228308379058	26.705415542949257	23.577606249418768
24-25	23.19445736073654	26.639697448773987	26.57986918379367	23.585976006695805
26-27	22.39685049133575	26.69921572274404	26.940698719737128	23.96323506618308
28-29	23.27071514926067	26.344586007005798	27.503332403360304	22.88136644037323
30-31	23.440590222883536	26.22647943209647	27.1744319414737	23.158498403546297
32-33	22.690101987042375	26.92333922316253	26.855761182925697	23.5307976068694
34-35	23.6104652965064	25.832480858055117	27.232710251402708	23.324343594035774
36-37	23.76143091850336	25.96081713630305	26.558789795095944	23.718962150097646
38-39	23.0614712173347	26.5938187792554	27.186831581884125	23.157878421525776
40-41	23.09836014755572	25.917418394866548	27.68684708143464	23.297374376143093
42-43	23.06240119036548	26.29653740041539	27.03865587897951	23.602405530239622
44-45	22.58966489971791	26.127282308813044	27.25967946929539	24.023373322173658
46-47	22.774109550823027	25.585418022877338	27.460553643944323	24.179918782355312
48-49	22.434669394587555	25.590997861062032	28.08487553860938	23.88945720574103
50-51	22.54688614030193	25.537679407297187	27.66018785455222	24.25524659784866
52-53	22.13552806968598	25.281626832821846	28.317988778325425	24.264856319166743
54-55	21.610403298304348	26.084813540407325	27.990328280479865	24.314454880808455
56-57	21.474317244799902	25.877429554542918	28.064726122942435	24.583527077714745
58-59	21.729439846244457	25.57270839145665	27.837192721411082	24.860659040887814
60-61	21.519885923308223	25.454291825537062	28.024737282618805	25.001084968535913
62-63	20.889984190458478	25.164760221953564	28.657428934560897	25.28782665302706
64-65	21.5973836758734	25.291236554139928	28.183762670882544	24.927617099104125
66-67	21.665891689141013	25.29836634737593	27.409405127251308	25.62633683623175
68-69	21.413868997799064	25.164760221953564	27.51945193589386	25.901918844353517
70-71	21.944883598375647	25.20908893642084	27.472333302334235	25.373694162869278
72-73	22.048420595802725	24.66474472240305	27.29873833658824	25.988096345205992
74-75	21.794227967388945	25.192659412877028	27.112433739421554	25.900678880312473
76	22.099879103505998	25.11857156142472	26.731144796800894	26.05040453826839
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.5
11	0.5
12	0.0
13	0.5
14	3.5
15	12.5
16	19.0
17	19.0
18	35.0
19	57.5
20	102.0
21	140.0
22	160.5
23	227.0
24	323.5
25	374.0
26	474.5
27	677.5
28	965.0
29	1150.0
30	1322.5
31	1665.0
32	2245.0
33	2655.0
34	2954.5
35	3705.0
36	4739.5
37	5323.0
38	5729.5
39	6789.5
40	8275.5
41	9515.5
42	9923.0
43	10286.5
44	10586.5
45	10620.5
46	10718.0
47	10410.0
48	9594.5
49	8562.0
50	8037.0
51	7542.5
52	6240.5
53	5039.5
54	4646.0
55	4388.0
56	3714.5
57	2877.5
58	2456.0
59	2196.5
60	1808.5
61	1588.5
62	1497.0
63	1413.0
64	1276.5
65	1216.0
66	1192.5
67	1177.0
68	1155.0
69	1171.5
70	1200.5
71	1191.0
72	1143.0
73	1085.0
74	1013.5
75	952.0
76	896.5
77	751.5
78	615.5
79	569.0
80	521.0
81	410.5
82	280.0
83	212.0
84	175.5
85	113.0
86	76.0
87	65.0
88	54.0
89	31.0
90	19.5
91	13.0
92	6.0
93	5.5
94	3.5
95	1.5
96	1.0
97	1.5
98	1.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	161295.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.72568895502031
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.46449436554303	36.75811401469357
2	12.956806570681268	13.144858799094827
3	5.2311227358283015	7.960569143494839
4	2.4200053777897286	4.910257602529526
5	1.394558654574788	3.5369974270746147
6	1.0963357696350435	3.336743234446201
7	0.7296682881517514	2.590904863758951
8	0.5817790706201569	2.3608915341455097
9	0.482778850619668	2.204036082953594
>10	2.6167835928524283	22.367091354350723
>50	0.025666723703830455	0.8295359434576398
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0024799280820856197	0.0
2	0.0	0.0	0.0	0.0024799280820856197	0.0
3	0.0	0.0	0.0	0.0024799280820856197	0.0
4	0.0	0.0	0.0	0.0030999101026070244	0.0
5	0.0	0.0	0.0	0.0030999101026070244	0.0
6	0.0	0.0	0.0	0.0030999101026070244	0.0
7	0.0	0.0	0.0	0.0030999101026070244	0.0
8	0.0	0.0	0.0	0.0030999101026070244	0.0
9	0.0	0.0	0.0	0.004339874143649834	0.0
10	0.0	0.0	0.0	0.0049598561641712394	0.0
11	0.0	0.0	0.0	0.0049598561641712394	0.0
12	0.0	0.0	0.0	0.0049598561641712394	0.0
13	0.0	0.0	0.0	0.0049598561641712394	0.0
14	0.0	0.0	0.0	0.0049598561641712394	0.0
15	0.0	0.0	0.0	0.0049598561641712394	0.0
16	0.0	0.0	0.0	0.0049598561641712394	0.0
17	0.0	0.0	0.0	0.0049598561641712394	0.0
18	0.0	0.0	0.0	0.0049598561641712394	0.0
19	0.0	0.0	0.0	0.005579838184692644	0.0
20	0.0	0.0	0.0	0.006199820205214049	0.0
21	0.0	0.0	0.0	0.006819802225735454	0.0
22	0.0	0.0	0.0	0.008059766266778264	0.0
23	0.0	0.0	0.0	0.008059766266778264	0.0
24	0.0	0.0	0.0	0.010539694348863883	0.0
25	0.0	0.0	0.0	0.010539694348863883	0.0
26	0.0	0.0	0.0	0.011159676369385288	0.0
27	0.0	0.0	0.0	0.014879568492513717	0.0
28	0.0	0.0	0.0	0.024799280820856195	0.0
29	0.0	0.0	0.0	0.03347902910815587	0.0
30	0.0	0.0	0.0	0.039678849313369915	0.0
31	0.0	0.0	0.0	0.04835859760066958	0.0
32	0.0	0.0	0.0	0.06695805821631173	0.0
33	0.0	0.0	0.0	0.08617750085247528	0.0
34	0.0	0.0	0.0	0.127096314206888	0.0
35	0.0	0.0	0.0	0.1673951455407793	0.0
36	0.0	0.0	0.0	0.23683313183917667	0.0
37	0.0	0.0	0.0	0.3378902011841657	0.0
38	0.0	0.0	0.0	0.47862611984252457	0.0
39	0.0	0.0	0.0	0.6640007439784247	0.0
40	0.0	0.0	0.0	0.8884342354071731	0.0
41	0.0	0.0	0.0	1.1184475650206145	0.0
42	0.0	0.0	0.0	1.4141789888093246	0.0
43	0.0	0.0	0.0	1.7582690101987042	0.0
44	0.0	0.0	0.0	2.211475867199851	0.0
45	0.0	0.0	0.0	2.741560494745652	0.0
46	0.0	0.0	0.0	3.387581760128956	0.0
47	0.0	0.0	0.0	4.031743079450696	0.0
48	0.0	0.0	0.0	4.639325459561673	0.0
49	0.0	0.0	0.0	5.253727641898385	0.0
50	0.0	0.0	0.0	5.778232431259493	0.0
51	0.0	0.0	0.0	6.31513686103103	0.0
52	0.0	0.0	0.0	6.865060913233516	0.0
53	0.0	0.0	0.0	7.426764623825909	0.0
54	0.0	0.0	0.0	8.007067795033944	0.0
55	0.0	0.0	0.0	8.665488700827677	0.0
56	0.0	0.0	0.0	9.351188815524349	0.0
57	0.0	0.0	0.0	10.00030999101026	0.0
58	0.0	0.0	0.0	10.668650609132335	0.0
59	0.0	0.0	0.0	11.321491676741374	0.0
60	0.0	0.0	0.0	11.987972348801884	0.0
61	0.0	0.0	0.0	12.570755448092005	0.0
62	0.0	0.0	0.0	13.13803899686909	0.0
63	0.0	0.0	0.0	13.719582132118168	0.0
64	0.0	0.0	0.0	14.329024458290709	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTCGCT	15	0.0022214276	70.0	45
GATCCAT	20	0.006941258	52.5	43
AGGACCG	20	0.006941258	52.5	5
ACTTAGA	20	0.006941258	52.5	27
ACGGGCT	20	0.006941258	52.5	40
CCCAACG	20	0.006941258	52.5	54
GGGAACG	20	0.006941258	52.5	68
GGAACGT	20	0.006941258	52.5	69
TAGATAA	20	0.006941258	52.5	16
GTCTTTC	20	0.006941258	52.5	8
CTCTCGC	20	0.006941258	52.5	44
CCTCTCG	30	5.8642955E-4	46.666664	43
GTTAAGC	35	0.0012525615	40.0	21
GTTCTAC	45	9.166753E-5	38.888885	35
CGTCCAA	45	9.166753E-5	38.888885	40
CGAACGA	40	0.0024132915	35.0	14
TGGTTCG	40	0.0024132915	35.0	35
CTTGCGG	40	0.0024132915	35.0	34
GTACGAA	40	0.0024132915	35.0	1
TCTTGGA	55	2.9821353E-4	31.818184	48
>>END_MODULE