FastQCFastQC Report
Wed 25 May 2016
SRR1781150_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781150_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146300
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGT15611.0669856459330143No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCG1770.12098427887901571No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTATA150.00222101570.048
TTCGTAC150.00222101570.064
ACTCGTA150.00222101570.038
CAGTCGT150.00222101570.01
ATCTCGT2400.061.2537
AGATCTC2450.060.035
TCTCGTA2400.059.79166438
CGCAGAT2550.059.01960432
CTCGTAT2850.058.9473739
CAGATCT2500.058.834
GATCTCG2500.058.836
CCGTCTT3000.058.33333247
CGTATGC2950.058.1355941
TCGTATG2950.058.1355940
GCCGTCT2950.058.1355946
TATGCCG3000.057.16666843
CGCAGAA252.384122E-456.00000432
TCGCAGA3250.055.99999631
CGTCTTC2950.055.7627148
GTATGCC3050.055.0819742
Produced by FastQC (version 0.11.5)