##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781150_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	146300
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.207060833902936	34.0	34.0	34.0	32.0	34.0
2	33.24195488721804	34.0	34.0	34.0	32.0	34.0
3	33.33293233082707	34.0	34.0	34.0	33.0	34.0
4	33.38359535201641	34.0	34.0	34.0	33.0	34.0
5	33.38687628161313	34.0	34.0	34.0	34.0	34.0
6	36.77094326725906	38.0	37.0	38.0	34.0	38.0
7	36.70501708817498	38.0	37.0	38.0	34.0	38.0
8	36.470123034859874	38.0	37.0	38.0	34.0	38.0
9	36.43531784005468	38.0	37.0	38.0	34.0	38.0
10-11	36.35933356117567	38.0	37.0	38.0	34.0	38.0
12-13	36.28277511961723	38.0	37.0	38.0	34.0	38.0
14-15	36.298045112781956	38.0	37.0	38.0	34.0	38.0
16-17	36.22777511961722	38.0	37.0	38.0	34.0	38.0
18-19	36.225095693779906	38.0	37.0	38.0	34.0	38.0
20-21	36.169039644565956	38.0	37.0	38.0	33.0	38.0
22-23	36.22813397129187	38.0	37.0	38.0	34.0	38.0
24-25	36.243831168831164	38.0	37.0	38.0	34.0	38.0
26-27	36.01794600136705	38.0	37.0	38.0	32.0	38.0
28-29	35.98262474367738	38.0	37.0	38.0	32.0	38.0
30-31	36.04512303485988	38.0	37.0	38.0	32.0	38.0
32-33	35.968885850991114	38.0	37.0	38.0	32.0	38.0
34-35	35.95972317156527	38.0	37.0	38.0	32.0	38.0
36-37	35.912286397812714	38.0	36.5	38.0	31.5	38.0
38-39	35.82547846889952	38.0	36.0	38.0	31.0	38.0
40-41	35.89816473000683	38.0	36.5	38.0	32.0	38.0
42-43	35.9253998632946	38.0	36.5	38.0	32.0	38.0
44-45	35.87580314422419	38.0	36.0	38.0	31.5	38.0
46-47	35.89913875598086	38.0	36.5	38.0	32.0	38.0
48-49	35.90767600820233	38.0	36.0	38.0	32.0	38.0
50-51	35.79504442925496	38.0	36.0	38.0	31.0	38.0
52-53	35.82334928229665	38.0	36.0	38.0	31.0	38.0
54-55	35.83245727956254	38.0	36.0	38.0	31.0	38.0
56-57	35.77935064935065	38.0	36.0	38.0	31.0	38.0
58-59	35.785352016404644	38.0	36.0	38.0	31.0	38.0
60-61	35.777036910457966	38.0	36.0	38.0	31.0	38.0
62-63	35.744309637730694	38.0	36.0	38.0	31.0	38.0
64-65	35.68727956254272	38.0	36.0	38.0	31.0	38.0
66-67	35.67609364319891	38.0	36.0	38.0	31.0	38.0
68-69	35.60393369788107	38.0	36.0	38.0	31.0	38.0
70-71	35.50085099111415	38.0	36.0	38.0	31.0	38.0
72-73	35.42430963773069	38.0	36.0	38.0	31.0	38.0
74-75	35.44326042378674	38.0	36.0	38.0	31.0	38.0
76	34.50595352016405	37.0	34.0	38.0	25.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	3.0
21	7.0
22	25.0
23	56.0
24	96.0
25	230.0
26	403.0
27	725.0
28	1253.0
29	2066.0
30	3052.0
31	4562.0
32	6414.0
33	9065.0
34	12218.0
35	16805.0
36	25817.0
37	63500.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.88926862611073	17.555707450444295	9.101161995898838	26.45386192754614
2	24.661654135338345	20.91045796308954	29.822282980177718	24.605604921394395
3	20.3568010936432	22.04101161995899	29.604921394395078	27.99726589200273
4	14.024606971975393	18.092959671907042	36.74572795625427	31.136705399863295
5	13.673274094326725	33.194121667805874	36.63431305536569	16.49829118250171
6	28.60218728639781	38.95352016404648	18.522214627477783	13.922077922077921
7	28.422419685577584	29.887901572112096	21.440874914559124	20.248803827751196
8	25.72043745727956	35.26110731373889	19.157894736842103	19.860560492139438
9	24.415584415584416	17.120984278879014	20.080656185919345	38.38277511961722
10-11	24.661995898838004	25.23069036226931	27.727272727272727	22.38004101161996
12-13	25.0228981544771	23.130553656869445	26.60287081339713	25.243677375256325
14-15	23.144565960355436	23.59876965140123	23.580656185919345	29.676008202323988
16-17	24.196855775803144	27.25085440874915	24.42071086807929	24.13157894736842
18-19	23.794600136705398	26.766575529733423	25.580997949419004	23.85782638414217
20-21	22.706766917293233	26.508544087491455	25.6596035543404	25.125085440874916
22-23	23.090567327409435	26.99350649350649	25.99521531100478	23.92071086807929
24-25	23.545796308954202	26.984278879015722	26.132604237867398	23.33732057416268
26-27	23.112781954887218	27.13192071086808	26.32775119617225	23.42754613807245
28-29	22.69788106630212	26.462747778537256	26.902255639097746	23.937115516062885
30-31	22.917976760082023	26.14217361585783	26.956596035543406	23.983253588516746
32-33	23.012303485987694	26.749487354750514	26.13568010936432	24.10252904989747
34-35	23.33697881066302	26.72693096377307	26.151059466848942	23.78503075871497
36-37	24.111756664388242	26.583048530416953	25.876965140123037	23.428229665071772
38-39	22.604921394395078	26.77272727272727	27.008885850991117	23.613465481886532
40-41	22.57928913192071	25.55775803144224	27.837662337662337	24.025290498974712
42-43	23.31852358168148	25.935064935064933	27.33526999316473	23.41114149008886
44-45	22.439507860560493	26.61449077238551	27.28298017771702	23.66302118933698
46-47	23.223855092276143	25.44053315105947	26.927204374572796	24.408407382091593
48-49	22.85645933014354	25.317156527682844	27.185235816814764	24.641148325358852
50-51	22.091592617908407	25.30451127819549	27.773069036226932	24.830827067669173
52-53	21.746753246753247	24.989747095010255	29.24436090225564	24.019138755980862
54-55	21.231373889268625	25.51537935748462	28.477785372522213	24.77546138072454
56-57	21.642857142857146	25.640806561859193	27.500683526999314	25.215652768284347
58-59	21.031442241968556	25.48838004101162	28.60423786739576	24.87593984962406
60-61	21.439166097060834	26.234791524265212	27.38004101161996	24.946001367054
62-63	20.028024606971975	26.742993848257008	27.907723855092275	25.321257689678745
64-65	20.40772385509228	26.9377990430622	26.913533834586467	25.74094326725906
66-67	20.449419002050583	26.813055365686942	26.784688995215312	25.95283663704716
68-69	20.295283663704716	27.284688995215312	26.2323991797676	26.18762816131237
70-71	20.772043745727956	26.38004101161996	26.173957621326043	26.673957621326043
72-73	20.641148325358852	25.471975393028025	26.14217361585783	27.744702665755298
74-75	20.54442925495557	25.7053998632946	25.969583048530414	27.78058783321941
76	21.13192071086808	25.55639097744361	25.76213260423787	27.549555707450445
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	1.0
10	2.0
11	1.5
12	1.0
13	3.0
14	4.0
15	12.5
16	27.0
17	32.0
18	48.0
19	64.5
20	92.5
21	120.0
22	137.0
23	209.0
24	313.5
25	363.0
26	476.0
27	634.5
28	829.5
29	979.0
30	1132.5
31	1431.0
32	1968.5
33	2361.0
34	2644.5
35	3277.5
36	4059.5
37	4492.0
38	5130.0
39	6331.0
40	7470.5
41	8305.5
42	8564.0
43	9128.5
44	9747.0
45	9913.0
46	10025.0
47	9890.5
48	9112.5
49	7825.5
50	7182.0
51	6670.5
52	5534.5
53	4588.5
54	4267.0
55	3954.0
56	3285.5
57	2531.0
58	2132.0
59	1880.5
60	1541.5
61	1352.5
62	1251.0
63	1219.0
64	1146.0
65	1127.5
66	1175.5
67	1201.0
68	1211.0
69	1177.5
70	1073.5
71	1013.0
72	1025.5
73	1016.0
74	931.0
75	868.0
76	811.0
77	696.0
78	593.5
79	549.0
80	477.0
81	353.0
82	255.5
83	210.0
84	173.0
85	112.0
86	68.5
87	49.0
88	43.0
89	24.0
90	12.0
91	9.5
92	6.0
93	3.5
94	1.0
95	1.5
96	2.0
97	1.0
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	146300.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.731373889268625
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.69140699980805	26.361585782638414
2	18.18894363043061	15.544771018455227
3	7.43969543796788	9.537252221462749
4	3.467912214473095	5.9275461380724535
5	2.0842664277944847	4.453178400546822
6	1.3100646234563953	3.358851674641148
7	1.0029432465288888	3.0
8	0.7454091752511357	2.5481886534518114
9	0.5934480772922133	2.2822966507177034
>10	3.4375199948813107	24.71701982228298
>50	0.0303922195917845	0.8462064251537936
>100	0.006398362019323054	0.35611756664388244
>500	0.0	0.0
>1k	0.0015995905048307635	1.0669856459330143
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGT	1561	1.0669856459330143	No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCG	177	0.12098427887901571	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.002050580997949419	0.0
2	0.0	0.0	0.0	0.002050580997949419	0.0
3	0.0	0.0	0.0	0.002050580997949419	0.0
4	0.0	0.0	0.0	0.002734107997265892	0.0
5	0.0	0.0	0.0	0.002734107997265892	0.0
6	0.0	0.0	0.0	0.002734107997265892	0.0
7	0.0	0.0	0.0	0.002734107997265892	0.0
8	0.0	0.0	0.0	0.002734107997265892	0.0
9	0.0	0.0	0.0	0.004101161995898838	0.0
10	0.0	0.0	0.0	0.004784688995215311	0.0
11	0.0	0.0	0.0	0.004784688995215311	0.0
12	0.0	0.0	0.0	0.004784688995215311	0.0
13	0.0	0.0	0.0	0.004784688995215311	0.0
14	0.0	0.0	0.0	0.004784688995215311	0.0
15	0.0	0.0	0.0	0.004784688995215311	0.0
16	0.0	0.0	0.0	0.004784688995215311	0.0
17	0.0	0.0	0.0	0.004784688995215311	0.0
18	0.0	0.0	0.0	0.004784688995215311	0.0
19	0.0	0.0	0.0	0.005468215994531784	0.0
20	0.0	0.0	0.0	0.006151742993848257	0.0
21	0.0	0.0	0.0	0.00683526999316473	0.0
22	0.0	0.0	0.0	0.00683526999316473	0.0
23	0.0	0.0	0.0	0.007518796992481203	0.0
24	0.0	0.0	0.0	0.009569377990430622	0.0
25	0.0	0.0	0.0	0.009569377990430622	0.0
26	0.0	0.0	0.0	0.010252904989747095	0.0
27	0.0	0.0	0.0	0.012987012987012988	0.0
28	0.0	0.0	0.0	0.02460697197539303	0.0
29	0.0	0.0	0.0	0.03417634996582365	0.0
30	0.0	0.0	0.0	0.041695146958304855	0.0
31	0.0	0.0	0.0	0.05194805194805195	0.0
32	0.0	0.0	0.0	0.0710868079289132	0.0
33	0.0	0.0	0.0	0.0936431989063568	0.0
34	0.0	0.0	0.0	0.14080656185919344	0.0
35	0.0	0.0	0.0	0.18660287081339713	0.0
36	0.0	0.0	0.0	0.2631578947368421	0.0
37	0.0	0.0	0.0	0.38072453861927547	0.0
38	0.0	0.0	0.0	0.5393028024606972	0.0
39	0.0	0.0	0.0	0.7361585782638415	0.0
40	0.0	0.0	0.0	0.9788106630211894	0.0
41	0.0	0.0	0.0	1.2303485987696514	0.0
42	0.0	0.0	0.0	1.5584415584415585	0.0
43	0.0	0.0	0.0	1.9316473000683527	0.0
44	0.0	0.0	0.0	2.421736158578264	0.0
45	0.0	0.0	0.0	3.0054682159945316	0.0
46	0.0	0.0	0.0	3.6855775803144226	0.0
47	0.0	0.0	0.0	4.388243335611756	0.0
48	0.0	0.0	0.0	5.028708133971292	0.0
49	0.0	0.0	0.0	5.700615174299385	0.0
50	0.0	0.0	0.0	6.252221462747778	0.0
51	0.0	0.0	0.0	6.817498291182502	0.0
52	0.0	0.0	0.0	7.398496240601504	0.0
53	0.0	0.0	0.0	8.015037593984962	0.0
54	0.0	0.0	0.0	8.63362952836637	0.0
55	0.0	0.0	0.0	9.32946001367054	0.0
56	0.0	0.0	0.0	10.033492822966506	0.0
57	0.0	0.0	0.0	10.74436090225564	0.0
58	0.0	0.0	0.0	11.444976076555024	0.0
59	0.0	0.0	0.0	12.150375939849624	0.0
60	0.0	0.0	0.0	12.829801777170198	0.0
61	0.0	0.0	0.0	13.436090225563909	0.0
62	0.0	0.0	0.0	14.03691045796309	0.0
63	0.0	0.0	0.0	14.630211893369788	0.0
64	0.0	0.0	0.0	15.257006151742994	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTGTATA	15	0.002221015	70.0	48
TTCGTAC	15	0.002221015	70.0	64
ACTCGTA	15	0.002221015	70.0	38
CAGTCGT	15	0.002221015	70.0	1
ATCTCGT	240	0.0	61.25	37
AGATCTC	245	0.0	60.0	35
TCTCGTA	240	0.0	59.791664	38
CGCAGAT	255	0.0	59.019604	32
CTCGTAT	285	0.0	58.94737	39
CAGATCT	250	0.0	58.8	34
GATCTCG	250	0.0	58.8	36
CCGTCTT	300	0.0	58.333332	47
CGTATGC	295	0.0	58.13559	41
TCGTATG	295	0.0	58.13559	40
GCCGTCT	295	0.0	58.13559	46
TATGCCG	300	0.0	57.166668	43
CGCAGAA	25	2.384122E-4	56.000004	32
TCGCAGA	325	0.0	55.999996	31
CGTCTTC	295	0.0	55.76271	48
GTATGCC	305	0.0	55.08197	42
>>END_MODULE