FastQCFastQC Report
Wed 25 May 2016
SRR1781149_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781149_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117743
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGACTT150.002219932270.000015
CATAGGG200.006936620552.52
AGACAAT200.006936620552.516
CCGAAAT200.006936620552.547
ATACGGT200.006936620552.56
TACCGGG200.006936620552.553
GTAGCAG305.857801E-446.6666689
CATGTAC305.857801E-446.6666683
TAGAACC350.001251182540.0000044
GCATGTA350.001251182540.0000042
GGAATCT400.002410650735.015
GGTGTTC400.002410650735.03
AAATAGC400.002410650735.053
GTACTGC501.7011902E-435.06
TGAATGC400.002410650735.03
GGTTCGA400.002410650735.044
GCAGAAA400.002410650735.048
GGTTACT400.002410650735.067
AGGATTG751.4768211E-632.6666685
TGTACTG552.9772733E-431.818185
Produced by FastQC (version 0.11.5)