##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781149_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	117743
Sequences flagged as poor quality	0
Sequence length	76
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.83519190100473	34.0	32.0	34.0	21.0	34.0
2	30.99234774041769	34.0	32.0	34.0	21.0	34.0
3	31.001647656336257	34.0	32.0	34.0	23.0	34.0
4	31.126546801083716	34.0	33.0	34.0	23.0	34.0
5	31.101704559931378	34.0	32.0	34.0	23.0	34.0
6	33.775825314456064	37.0	34.0	38.0	23.0	38.0
7	33.78802136857393	37.0	34.0	38.0	22.0	38.0
8	33.518196410826974	37.0	34.0	38.0	21.0	38.0
9	33.86986912173123	37.0	34.0	38.0	23.0	38.0
10-11	33.67713154922161	37.0	34.0	38.0	22.0	38.0
12-13	33.64837824753913	37.0	34.0	38.0	22.0	38.0
14-15	33.50585172791588	37.0	34.0	38.0	21.0	38.0
16-17	33.44765293902822	37.0	34.0	38.0	21.0	38.0
18-19	33.50205107734642	37.0	34.0	38.0	21.5	38.0
20-21	33.42062797788404	37.0	34.0	38.0	21.0	38.0
22-23	33.474053659240894	37.0	34.0	38.0	21.0	38.0
24-25	33.48706080191604	37.0	34.0	38.0	21.0	38.0
26-27	33.39956515461641	37.0	34.0	38.0	21.0	38.0
28-29	33.47632555650867	37.0	34.0	38.0	21.0	38.0
30-31	33.54655053803623	37.0	34.0	38.0	21.5	38.0
32-33	33.49870480623052	37.0	34.0	38.0	21.5	38.0
34-35	33.48744723677841	37.0	34.0	38.0	21.0	38.0
36-37	33.3478635672609	37.0	34.0	38.0	21.0	38.0
38-39	33.37596714879016	37.0	34.0	38.0	21.0	38.0
40-41	33.368782008272255	37.0	34.0	38.0	21.0	38.0
42-43	33.43149486593683	37.0	34.0	38.0	21.0	38.0
44-45	33.47962086918118	37.0	34.0	38.0	21.5	38.0
46-47	33.46841850470941	37.0	34.0	38.0	21.5	38.0
48-49	33.44214093406827	37.0	34.0	38.0	21.0	38.0
50-51	33.303134793575836	37.0	34.0	38.0	21.0	38.0
52-53	33.37542783859762	37.0	34.0	38.0	21.0	38.0
54-55	33.3416041717979	37.0	34.0	38.0	21.0	38.0
56-57	33.31559413298455	37.0	34.0	38.0	21.0	38.0
58-59	33.314834002870654	37.0	34.0	38.0	21.0	38.0
60-61	33.259752172103646	37.0	34.0	38.0	21.0	38.0
62-63	33.25432934441963	37.0	34.0	38.0	21.0	38.0
64-65	33.2532464774976	37.0	34.0	38.0	21.0	38.0
66-67	33.131859218807065	37.0	33.5	38.0	21.0	38.0
68-69	32.9241695896996	37.0	32.5	38.0	20.0	38.0
70-71	33.07404261824482	37.0	33.0	38.0	21.0	38.0
72-73	33.06079342296357	37.0	33.0	38.0	21.0	38.0
74-75	33.05524319917107	37.0	32.0	38.0	21.0	38.0
76	31.685255174405274	36.0	28.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	6.0
13	23.0
14	99.0
15	277.0
16	592.0
17	1091.0
18	1435.0
19	1504.0
20	1441.0
21	1376.0
22	1312.0
23	1461.0
24	1618.0
25	1866.0
26	2158.0
27	2497.0
28	2896.0
29	3403.0
30	3997.0
31	4822.0
32	5616.0
33	6651.0
34	8579.0
35	11131.0
36	16701.0
37	35189.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.91100957169429	18.6533382026957	9.26254639341617	26.173105832193844
2	22.660370467883443	19.006650076862318	32.537815411531895	25.795164043722345
3	20.542197837663387	21.132466473590785	28.29382638458337	30.03150930416246
4	14.72614083215138	15.660378960957338	34.7477132398529	34.86576696703838
5	15.204300892622067	31.935656472146963	34.656837349141774	18.203205286089194
6	32.48261043119336	34.64664566046389	16.926696279184323	15.944047629158423
7	29.79455254240167	27.361286870557063	21.447559515215342	21.396601071825927
8	26.43808973781881	33.06523530061235	19.23086722777575	21.26580773379309
9	24.667283830036606	14.321870514595346	20.988084217320775	40.02276143804727
10-11	27.036426793949538	22.920683182864373	26.11917481294005	23.923715210246044
12-13	26.30644709239615	21.10274071494696	25.773082051586933	26.817730141069955
14-15	24.612928157087897	22.437002624359835	22.60813806340929	30.34193115514298
16-17	24.974308451457837	25.08514306582982	23.995057030991227	25.94549145172112
18-19	24.187849808481186	24.70889989213796	25.19045718216794	25.912793117212914
20-21	24.6095309275286	24.778543098103498	25.01295193769481	25.598974036673095
22-23	24.8507342262385	24.171712968074534	25.15733419396482	25.82021861172214
24-25	24.54073702895289	24.350916827327314	24.949678537152952	26.158667606566844
26-27	24.146233746379828	24.570887441291628	25.339510629081985	25.943368183246562
28-29	24.66133867830784	24.23668498339604	25.620631375113597	25.481344963182522
30-31	24.752214569018964	24.433299644140202	25.130156357490467	25.684329429350367
32-33	24.52927137919027	24.80274835871347	25.061787112609668	25.606193149486593
34-35	25.146293197897112	23.864688346653303	25.401510068539107	25.587508386910475
36-37	24.810816778916795	24.053234587194144	25.134827548134496	26.00112108575457
38-39	24.250273901633218	24.150055629634036	26.055052105008365	25.544618363724382
40-41	24.404423192886203	24.13009690597318	26.117051544465486	25.34842835667513
42-43	24.059179738922907	23.885921031398894	25.865231903382792	26.189667326295407
44-45	23.385678978792797	24.23201379275201	26.32003601063333	26.062271217821863
46-47	23.6485396159432	24.228191909497802	26.39562436832763	25.727644106231367
48-49	23.26465267574293	24.043467552211172	26.66145758134241	26.030422190703483
50-51	23.3822817492335	24.51270988508871	26.121722735109522	25.983285630568272
52-53	23.276967632895374	24.291465310039662	26.52386978419099	25.90769727287397
54-55	22.8387250197464	24.47703897471612	26.42917201022566	26.255063995311822
56-57	22.95093105717986	24.40877338259337	26.464530649510586	26.175764910716182
58-59	22.997120847948498	24.200589419328537	26.520047900936788	26.28224183178618
60-61	22.77332835072998	24.353464749496787	26.4270487417511	26.446158158022133
62-63	22.55217954434465	24.10514470136102	26.74055672335818	26.602119030936155
64-65	22.972163832091216	24.14421300719791	26.273435675308406	26.610187485402463
66-67	22.934369492749006	24.398581650635922	25.655986580886257	27.011062275728815
68-69	22.67617894982695	24.576936959891288	25.826698091173533	26.920185999108227
70-71	23.112822951877835	24.788520663824293	25.702807834077902	26.395848550219974
72-73	23.28480915900868	24.49338383924173	25.616602401861698	26.605204599887887
74-75	23.145096906796216	24.630972804946406	25.60343802551341	26.620492262743966
76	22.8208895645601	24.70889989213796	25.31955190542113	27.15065863788081
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	3.5
15	9.5
16	22.0
17	32.0
18	43.0
19	60.5
20	80.5
21	94.0
22	147.0
23	234.5
24	348.5
25	428.0
26	490.5
27	612.5
28	752.5
29	833.0
30	984.0
31	1208.5
32	1566.0
33	1850.0
34	2044.5
35	2434.0
36	2985.0
37	3341.0
38	3579.5
39	4122.0
40	4733.0
41	5287.0
42	5534.0
43	5635.5
44	5769.5
45	5687.0
46	5572.0
47	5575.5
48	5380.5
49	4905.0
50	4628.0
51	4483.0
52	4048.0
53	3650.0
54	3542.0
55	3386.0
56	3150.5
57	2928.5
58	2786.0
59	2758.5
60	2693.5
61	2658.5
62	2661.0
63	2620.5
64	2544.5
65	2444.0
66	2272.0
67	2165.0
68	2070.0
69	1905.5
70	1711.0
71	1586.0
72	1508.5
73	1334.0
74	1125.0
75	1013.0
76	968.0
77	815.5
78	606.5
79	505.0
80	459.0
81	340.5
82	227.0
83	186.0
84	152.5
85	83.5
86	42.0
87	36.0
88	25.5
89	13.0
90	7.0
91	3.5
92	4.0
93	2.5
94	1.0
95	0.5
96	0.0
97	0.0
98	1.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	4.246536949117994E-4
58-59	0.0
60-61	0.0
62-63	4.246536949117994E-4
64-65	4.246536949117994E-4
66-67	4.246536949117994E-4
68-69	4.246536949117994E-4
70-71	8.493073898235988E-4
72-73	8.493073898235988E-4
74-75	8.493073898235988E-4
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	117743.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.17063434768946
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.91602468438673	46.693221677721816
2	14.583417362427568	18.424874514833153
3	5.670955511636349	10.74713571082782
4	2.307102811277377	5.829645923749183
5	1.0863281302518184	3.4312018548873393
6	0.6399655816830019	2.4256219053361985
7	0.42485110044501806	1.8786679462898006
8	0.266204170532005	1.3453029054805805
9	0.2312480673308326	1.314727839446931
>10	0.872558114521572	7.863737122376701
>50	0.001344465507737399	0.04586259905047434
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.004246536949117995	0.0
2	0.0	0.0	0.0	0.004246536949117995	0.0
3	0.0	0.0	0.0	0.005095844338941593	0.0
4	0.0	0.0	0.0	0.005945151728765192	0.0
5	0.0	0.0	0.0	0.005945151728765192	0.0
6	0.0	0.0	0.0	0.005945151728765192	0.0
7	0.0	0.0	0.0	0.005945151728765192	0.0
8	0.0	0.0	0.0	0.005945151728765192	0.0
9	0.0	0.0	0.0	0.005945151728765192	0.0
10	0.0	0.0	0.0	0.006794459118588791	0.0
11	0.0	0.0	0.0	0.00764376650841239	0.0
12	0.0	0.0	0.0	0.00764376650841239	0.0
13	0.0	0.0	0.0	0.00764376650841239	0.0
14	0.0	0.0	0.0	0.00849307389823599	0.0
15	0.0	0.0	0.0	0.009342381288059587	0.0
16	0.0	0.0	0.0	0.009342381288059587	0.0
17	0.0	0.0	0.0	0.010191688677883186	0.0
18	0.0	0.0	0.0	0.010191688677883186	0.0
19	0.0	0.0	8.493073898235988E-4	0.012739610847353982	0.0
20	0.0	0.0	8.493073898235988E-4	0.01443822562700118	0.0
21	0.0	0.0	8.493073898235988E-4	0.01443822562700118	0.0
22	0.0	0.0	8.493073898235988E-4	0.020383377355766372	0.0
23	0.0	0.0	8.493073898235988E-4	0.020383377355766372	0.0
24	0.0	0.0	8.493073898235988E-4	0.02293129952523717	0.0
25	0.0	0.0	8.493073898235988E-4	0.024629914304884367	0.0
26	0.0	0.0	8.493073898235988E-4	0.024629914304884367	0.0
27	0.0	0.0	8.493073898235988E-4	0.03652021776241475	0.0
28	0.0	0.0	8.493073898235988E-4	0.05265705816906313	0.0
29	0.0	0.0	8.493073898235988E-4	0.0798348946434183	0.0
30	0.0	0.0	8.493073898235988E-4	0.09936896460936107	0.0
31	0.0	0.0	8.493073898235988E-4	0.12569749369389263	0.0
32	0.0	0.0	8.493073898235988E-4	0.1477794858293062	0.0
33	0.0	0.0	8.493073898235988E-4	0.19024485532048616	0.0
34	0.0	0.0	8.493073898235988E-4	0.2734769795231988	0.0
35	0.0	0.0	8.493073898235988E-4	0.3821883254206195	0.0
36	0.0	0.0	8.493073898235988E-4	0.5401594999278089	0.0
37	0.0	0.0	8.493073898235988E-4	0.7991982538240066	0.0
38	0.0	0.0	8.493073898235988E-4	1.0760724629064997	0.0
39	0.0	0.0	8.493073898235988E-4	1.3368098315823447	0.0
40	0.0	0.0	8.493073898235988E-4	1.622177114563074	0.0
41	0.0	0.0	8.493073898235988E-4	1.9440646153062178	0.0
42	0.0	0.0	8.493073898235988E-4	2.2897327229644224	0.0
43	0.0	0.0	8.493073898235988E-4	2.6583321301478646	0.0
44	0.0	0.0	8.493073898235988E-4	3.063451755093721	0.0
45	0.0	0.0	8.493073898235988E-4	3.4770644539378135	0.0
46	0.0	0.0	8.493073898235988E-4	3.918704296646085	0.0
47	0.0	0.0	8.493073898235988E-4	4.374782364981358	0.0
48	0.0	0.0	8.493073898235988E-4	4.837654892435219	0.0
49	0.0	0.0	8.493073898235988E-4	5.342143481990437	0.0
50	0.0	0.0	8.493073898235988E-4	5.771043713851355	0.0
51	0.0	0.0	8.493073898235988E-4	6.154930654051621	0.0
52	0.0	0.0	8.493073898235988E-4	6.577036426793949	0.0
53	0.0	0.0	8.493073898235988E-4	6.979608129570336	0.0
54	0.0	0.0	8.493073898235988E-4	7.4034125170923115	0.0
55	0.0	0.0	8.493073898235988E-4	7.906202491867882	0.0
56	0.0	0.0	8.493073898235988E-4	8.421732077490807	0.0
57	0.0	0.0	8.493073898235988E-4	8.843837850233134	0.0
58	0.0	0.0	8.493073898235988E-4	9.28632700033123	0.0
59	0.0	0.0	8.493073898235988E-4	9.669364633141672	0.0
60	0.0	0.0	8.493073898235988E-4	10.068539106358765	0.0
61	0.0	0.0	8.493073898235988E-4	10.470261501745327	0.0
62	0.0	0.0	8.493073898235988E-4	10.888120737538538	0.0
63	0.0	0.0	8.493073898235988E-4	11.290692440314924	0.0
64	0.0	0.0	8.493073898235988E-4	11.664387691837307	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGGACTT	15	0.0022199322	70.00001	5
CATAGGG	20	0.0069366205	52.5	2
AGACAAT	20	0.0069366205	52.5	16
CCGAAAT	20	0.0069366205	52.5	47
ATACGGT	20	0.0069366205	52.5	6
TACCGGG	20	0.0069366205	52.5	53
GTAGCAG	30	5.857801E-4	46.666668	9
CATGTAC	30	5.857801E-4	46.666668	3
TAGAACC	35	0.0012511825	40.000004	4
GCATGTA	35	0.0012511825	40.000004	2
GGAATCT	40	0.0024106507	35.0	15
GGTGTTC	40	0.0024106507	35.0	3
AAATAGC	40	0.0024106507	35.0	53
GTACTGC	50	1.7011902E-4	35.0	6
TGAATGC	40	0.0024106507	35.0	3
GGTTCGA	40	0.0024106507	35.0	44
GCAGAAA	40	0.0024106507	35.0	48
GGTTACT	40	0.0024106507	35.0	67
AGGATTG	75	1.4768211E-6	32.666668	5
TGTACTG	55	2.9772733E-4	31.81818	5
>>END_MODULE