FastQCFastQC Report
Wed 25 May 2016
SRR1781149_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781149_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106262
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGT34093.2081082607140843No Hit
TCTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCG3460.3256102840149819No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCC2210.20797651088818206No Hit
CTTATACACATCTCCGAGCCCACGAGACAACGCCATACCTCGTATGCCGT2190.20609437051815324No Hit
CTTATACACATCTCCGAGCCCACGAGACAACGCCATAACTCGTATGCCGT1290.12139805386685738No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATATCG150.002219335970.0000147
TACGGAC207.895599E-570.038
TCGTATG4750.060.42105540
CGTATGC4700.060.3191541
CTCGTAT4650.060.21505739
GTATGCC4800.059.06250442
CGTCTTC4650.058.70968248
ATATCTC4300.058.60465235
TATCTCG4300.058.60465236
TCTCGTA4300.058.60465238
TATGCCG4850.058.4536143
GCCGTCT4750.058.2105346
CCGTCTT4700.058.08510647
ATGCCGT4950.057.27272844
ATCTCGT4400.057.27272437
GTCTTCT4750.056.73684349
TCTTCTG4800.056.14583650
TTATGAC252.3811404E-455.99999650
ACTTATG252.3811404E-455.99999648
TCTGCTT4950.055.8585953
Produced by FastQC (version 0.11.5)