##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781149_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	106262
Sequences flagged as poor quality	0
Sequence length	76
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.242137358604204	34.0	34.0	34.0	32.0	34.0
2	33.3037115808097	34.0	34.0	34.0	33.0	34.0
3	33.36893715533304	34.0	34.0	34.0	33.0	34.0
4	33.42781050610755	34.0	34.0	34.0	34.0	34.0
5	33.4216841392031	34.0	34.0	34.0	34.0	34.0
6	36.83607498447234	38.0	37.0	38.0	34.0	38.0
7	36.73355479851687	38.0	37.0	38.0	34.0	38.0
8	36.5363347198434	38.0	37.0	38.0	34.0	38.0
9	36.50629575953775	38.0	37.0	38.0	34.0	38.0
10-11	36.43405450678512	38.0	37.0	38.0	34.0	38.0
12-13	36.361093335340954	38.0	37.0	38.0	34.0	38.0
14-15	36.358731249176564	38.0	37.0	38.0	34.0	38.0
16-17	36.28323859893471	38.0	37.0	38.0	34.0	38.0
18-19	36.255001788033354	38.0	37.0	38.0	34.0	38.0
20-21	36.20562854077657	38.0	37.0	38.0	33.5	38.0
22-23	36.28052831680186	38.0	37.0	38.0	34.0	38.0
24-25	36.2682426455365	38.0	37.0	38.0	34.0	38.0
26-27	36.07671604148237	38.0	37.0	38.0	32.5	38.0
28-29	36.043053960964414	38.0	37.0	38.0	32.0	38.0
30-31	36.09983813592818	38.0	37.0	38.0	33.0	38.0
32-33	35.99648510285897	38.0	37.0	38.0	32.0	38.0
34-35	35.99046225367488	38.0	37.0	38.0	32.0	38.0
36-37	35.94058553386911	38.0	36.5	38.0	32.0	38.0
38-39	35.72189964427547	38.0	36.0	38.0	31.0	38.0
40-41	35.90352618998325	38.0	36.5	38.0	31.5	38.0
42-43	35.979169411454706	38.0	37.0	38.0	32.0	38.0
44-45	35.95197248310779	38.0	36.5	38.0	32.0	38.0
46-47	35.957943573431706	38.0	37.0	38.0	32.0	38.0
48-49	35.93657657488096	38.0	36.5	38.0	32.0	38.0
50-51	35.831816641885155	38.0	36.0	38.0	31.0	38.0
52-53	35.837533643259114	38.0	36.0	38.0	31.0	38.0
54-55	35.83675726035648	38.0	36.0	38.0	31.0	38.0
56-57	35.779479023545576	38.0	36.0	38.0	31.0	38.0
58-59	35.740843387099815	38.0	36.0	38.0	31.0	38.0
60-61	35.743365455195644	38.0	36.0	38.0	31.0	38.0
62-63	35.73381829816868	38.0	36.0	38.0	31.0	38.0
64-65	35.689409196137845	38.0	36.0	38.0	31.0	38.0
66-67	35.61492349099396	38.0	36.0	38.0	31.0	38.0
68-69	35.46569799175623	38.0	36.0	38.0	31.0	38.0
70-71	35.15924319135721	38.0	36.0	38.0	28.0	38.0
72-73	34.93566844215242	38.0	36.0	38.0	27.0	38.0
74-75	34.946984811127216	38.0	36.0	38.0	27.0	38.0
76	34.085524458414106	37.0	34.0	38.0	24.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	6.0
22	28.0
23	44.0
24	95.0
25	163.0
26	340.0
27	612.0
28	1011.0
29	1522.0
30	2292.0
31	3391.0
32	4844.0
33	6401.0
34	8809.0
35	12194.0
36	17509.0
37	47000.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.63271912819258	17.712823022341006	8.956164950782028	29.698292898684386
2	22.129265400613576	18.46285595979748	34.16367092657771	25.244207713011235
3	20.621670964220513	20.174662626338673	30.76734862886074	28.43631778058008
4	14.481188007001562	18.65765748809546	33.77971429109183	33.08144021381115
5	14.809621501571588	32.21659671378291	36.729969321111966	16.24381246353353
6	32.011443413449776	37.51105757467392	15.879618301932958	14.597880709943349
7	28.593476501477483	27.465133349645214	19.620372287365193	24.32101786151211
8	25.87001938604581	36.30836987822551	17.155709472812482	20.66590126291619
9	24.01517005138243	13.904311983587736	19.169599668743295	42.91091829628654
10-11	26.28503133763716	24.585458583501158	26.21915642468615	22.910353654175527
12-13	25.379721819653312	20.449925655455385	26.510888182040617	27.659464342850693
14-15	23.81236942651183	21.20983982985451	21.70531328226459	33.27247746136907
16-17	26.655812990532834	26.042235229903447	22.18808228717698	25.11386949238674
18-19	26.450659690199696	24.215147465697992	23.191263104402328	26.142929739699987
20-21	24.127157403399146	23.882008620202893	23.068923980350455	28.921909996047507
22-23	24.16103593005966	25.455948504639476	23.33712898307956	27.0458865822213
24-25	26.381961566693647	25.849315841975496	23.05010257665017	24.718620014680695
26-27	25.95518623778961	26.045528975550997	23.331953097061977	24.66733168959741
28-29	24.004818279347273	25.71380173533342	23.917298752140933	26.36408123317837
30-31	24.065046771188197	26.098228905911803	23.352186106039788	26.484538216860216
32-33	25.814025710037452	23.823662268732	23.514520712954774	26.84779130827577
34-35	24.147860947469464	25.273380888746683	23.89988895371817	26.678869210065688
36-37	24.187856430332573	25.60651973424178	25.550055523140912	24.655568312284732
38-39	23.754493610133444	24.117276166456495	25.890722930116127	26.237507293293934
40-41	23.562044757297997	23.078334682200598	26.80309047448759	26.55653008601381
42-43	25.868137245675783	23.2223184205078	26.105757467391914	24.8037868664245
44-45	23.32865935141443	25.279968380041783	26.85343772938586	24.53793453915793
46-47	25.405601249741206	23.30795580734411	24.812256498089628	26.474186444825055
48-49	25.01740979842277	23.083510568218177	25.07340347443112	26.82567615892793
50-51	22.721669082080144	23.32865935141443	27.164461425533116	26.785210140972314
52-53	22.33253656057669	23.015753514897142	29.54066364269447	25.1110462818317
54-55	22.19937512939715	23.332423632154487	27.40302271743427	27.0651785210141
56-57	24.016581656659955	23.22608270124786	25.48935649620749	27.267979145884702
58-59	22.212079576894844	23.17855865690463	29.209406937569405	25.39995482863112
60-61	24.562402363968307	25.014116052775215	24.99200090342738	25.4314806798291
62-63	21.491690350266325	27.969546968812935	24.91906796408876	25.61969471683198
64-65	21.977282565733752	28.437729385857597	24.076810148500876	25.508177899907775
66-67	21.676140106529147	28.5228962376014	23.78037304022134	26.020590615648114
68-69	21.539214394609548	28.946377820857876	23.529577835915003	25.984829948617566
70-71	22.22948937531761	27.619468859987578	24.071634262483297	26.079407502211517
72-73	21.810713142986206	26.076584291656474	24.503585477404904	27.609117087952416
74-75	21.87188270501214	26.041764694810936	24.4052436430709	27.68110895710602
76	22.1960813837496	25.936835369181832	24.14127345617436	27.725809790894207
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	2.5
12	5.0
13	3.5
14	7.0
15	9.5
16	12.5
17	18.0
18	43.5
19	76.5
20	96.5
21	109.0
22	130.0
23	192.5
24	299.5
25	365.0
26	421.0
27	521.5
28	707.5
29	849.0
30	949.5
31	1043.0
32	1329.0
33	1622.0
34	1753.0
35	2048.0
36	2506.5
37	2801.0
38	2898.5
39	3376.5
40	4259.0
41	5206.0
42	5651.0
43	5955.5
44	6144.5
45	5762.0
46	5495.0
47	5358.0
48	4770.0
49	4110.5
50	3902.0
51	3672.5
52	3153.5
53	2868.0
54	2872.0
55	2762.0
56	2617.5
57	2504.5
58	2426.0
59	2358.5
60	2375.0
61	2313.5
62	2168.0
63	2300.5
64	2335.0
65	2247.5
66	2153.0
67	2048.0
68	1933.0
69	1739.5
70	1609.0
71	1557.0
72	1421.0
73	1274.5
74	1130.5
75	997.0
76	922.5
77	746.5
78	551.5
79	458.0
80	433.5
81	330.5
82	202.5
83	153.0
84	132.0
85	88.0
86	46.0
87	27.0
88	21.0
89	14.5
90	8.5
91	2.0
92	1.0
93	0.5
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	106262.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.657582202480654
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.043517111964974	33.19719184656792
2	20.76490036636583	21.86858895936459
3	8.05468680189438	12.72420997157968
4	3.2115092485032615	6.764412489883495
5	1.5405236350638907	4.056012497412057
6	0.8524707354123849	2.6933428695112083
7	0.5861853274953087	2.1606971447930587
8	0.4467875971763024	1.8821403700287966
9	0.3181127691895273	1.5075944363930662
>10	1.1687963542132072	8.951459599856957
>50	0.003574300777410419	0.12516233460691498
>100	0.007148601554820838	0.8610792192881744
>500	0.0	0.0
>1k	0.0017871503887052094	3.2081082607140843
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGT	3409	3.2081082607140843	No Hit
TCTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCG	346	0.3256102840149819	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCC	221	0.20797651088818206	No Hit
CTTATACACATCTCCGAGCCCACGAGACAACGCCATACCTCGTATGCCGT	219	0.20609437051815324	No Hit
CTTATACACATCTCCGAGCCCACGAGACAACGCCATAACTCGTATGCCGT	129	0.12139805386685738	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0037642807400575934	0.0
2	0.0	0.0	0.0	0.0037642807400575934	0.0
3	0.0	0.0	0.0	0.004705350925071992	0.0
4	0.0	0.0	0.0	0.00564642111008639	0.0
5	0.0	0.0	0.0	0.00564642111008639	0.0
6	0.0	0.0	0.0	0.00564642111008639	0.0
7	0.0	0.0	0.0	0.00564642111008639	0.0
8	0.0	0.0	0.0	0.00564642111008639	0.0
9	0.0	0.0	0.0	0.00564642111008639	0.0
10	0.0	0.0	0.0	0.006587491295100788	0.0
11	0.0	0.0	0.0	0.007528561480115187	0.0
12	0.0	0.0	0.0	0.007528561480115187	0.0
13	0.0	0.0	0.0	0.007528561480115187	0.0
14	0.0	0.0	0.0	0.008469631665129585	0.0
15	0.0	0.0	0.0	0.008469631665129585	0.0
16	0.0	0.0	0.0	0.008469631665129585	0.0
17	0.0	0.0	0.0	0.009410701850143983	0.0
18	0.0	0.0	0.0	0.009410701850143983	0.0
19	0.0	0.0	0.0	0.009410701850143983	0.0
20	0.0	0.0	0.0	0.01129284222017278	0.0
21	0.0	0.0	0.0	0.01129284222017278	9.410701850143983E-4
22	0.0	0.0	0.0	0.012233912405187179	9.410701850143983E-4
23	0.0	0.0	0.0	0.013174982590201577	9.410701850143983E-4
24	0.0	0.0	0.0	0.015057122960230374	9.410701850143983E-4
25	0.0	0.0	0.0	0.01693926333025917	9.410701850143983E-4
26	0.0	0.0	0.0	0.01693926333025917	9.410701850143983E-4
27	0.0	0.0	0.0	0.02917317573544635	9.410701850143983E-4
28	0.0	0.0	0.0	0.04611243906570552	9.410701850143983E-4
29	0.0	0.0	0.0	0.07246240424610867	9.410701850143983E-4
30	0.0	0.0	0.0	0.09693022905648303	9.410701850143983E-4
31	0.0	0.0	0.0	0.12516233460691498	9.410701850143983E-4
32	0.0	0.0	0.0	0.15245336997233253	9.410701850143983E-4
33	0.0	0.0	0.0	0.20515330033313883	9.410701850143983E-4
34	0.0	0.0	0.0	0.2983192486495643	9.410701850143983E-4
35	0.0	0.0	0.0	0.4197173025164217	9.410701850143983E-4
36	0.0	0.0	0.0	0.5900510060040278	9.410701850143983E-4
37	0.0	0.0	0.0	0.8723720615083473	9.410701850143983E-4
38	0.0	0.0	0.0	1.1753966610829836	9.410701850143983E-4
39	0.0	0.0	0.0	1.4614819973273607	9.410701850143983E-4
40	0.0	0.0	0.0	1.7626244565319682	9.410701850143983E-4
41	0.0	0.0	0.0	2.1192900566524253	9.410701850143983E-4
42	0.0	0.0	0.0	2.491953849918127	9.410701850143983E-4
43	0.0	0.0	0.0	2.889085467994203	9.410701850143983E-4
44	0.0	0.0	0.0	3.314449191620711	9.410701850143983E-4
45	0.0	0.0	0.0	3.768045020797651	9.410701850143983E-4
46	0.0	0.0	0.0	4.22352299034462	9.410701850143983E-4
47	0.0	0.0	0.0	4.70723306544202	9.410701850143983E-4
48	0.0	0.0	0.0	5.196589561649508	9.410701850143983E-4
49	0.0	0.0	0.0	5.732058496922701	9.410701850143983E-4
50	0.0	0.0	0.0	6.165891852214338	9.410701850143983E-4
51	0.0	0.0	0.0	6.5677288212154865	9.410701850143983E-4
52	0.0	0.0	0.0	7.016619299467354	9.410701850143983E-4
53	0.0	0.0	0.0	7.435395531798761	9.410701850143983E-4
54	0.0	0.0	0.0	7.894637782085788	9.410701850143983E-4
55	0.0	0.0	0.0	8.417872804953793	9.410701850143983E-4
56	0.0	0.0	0.0	8.951459599856957	9.410701850143983E-4
57	0.0	0.0	0.0	9.409760779958969	9.410701850143983E-4
58	0.0	0.0	0.0	9.882178012836198	9.410701850143983E-4
59	0.0	0.0	0.0	10.30660066627769	9.410701850143983E-4
60	0.0	0.0	0.0	10.7385518811993	9.410701850143983E-4
61	0.0	0.0	0.0	11.158269183715722	9.410701850143983E-4
62	0.0	0.0	0.0	11.604336451412546	9.410701850143983E-4
63	0.0	0.0	0.0	12.02687696448401	9.410701850143983E-4
64	0.0	0.0	0.0	12.423067512375074	9.410701850143983E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATATCG	15	0.0022193359	70.00001	47
TACGGAC	20	7.895599E-5	70.0	38
TCGTATG	475	0.0	60.421055	40
CGTATGC	470	0.0	60.31915	41
CTCGTAT	465	0.0	60.215057	39
GTATGCC	480	0.0	59.062504	42
CGTCTTC	465	0.0	58.709682	48
ATATCTC	430	0.0	58.604652	35
TATCTCG	430	0.0	58.604652	36
TCTCGTA	430	0.0	58.604652	38
TATGCCG	485	0.0	58.45361	43
GCCGTCT	475	0.0	58.21053	46
CCGTCTT	470	0.0	58.085106	47
ATGCCGT	495	0.0	57.272728	44
ATCTCGT	440	0.0	57.272724	37
GTCTTCT	475	0.0	56.736843	49
TCTTCTG	480	0.0	56.145836	50
TTATGAC	25	2.3811404E-4	55.999996	50
ACTTATG	25	2.3811404E-4	55.999996	48
TCTGCTT	495	0.0	55.85859	53
>>END_MODULE