FastQCFastQC Report
Wed 25 May 2016
SRR1781148_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781148_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences155686
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAT150.002221282370.037
CGCGATA150.002221282370.038
ACTGATC252.3845978E-455.99999624
CTTCGTG200.00694080752.57
GAGTCGA200.00694080752.558
GTGCGTT200.00694080752.531
ATTTGCG200.00694080752.536
TACTGAT352.0790874E-550.00000423
CGCAGTA305.863664E-446.66666418
CTACCCG305.863664E-446.66666410
CATGTAA305.863664E-446.66666435
CGCAAAG305.863664E-446.66666412
CCGCAGT305.863664E-446.66666417
TACCCGC350.001252427140.00000411
CCGTGCG400.002413034735.09
GCAGTAC450.004297135431.11111319
CACTACG450.004297135431.11111345
GTATAGA450.004297135431.1111131
GTCCAAG802.4505553E-630.6251
CCTATAC604.964754E-429.1666663
Produced by FastQC (version 0.11.5)