##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781148_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	155686
Sequences flagged as poor quality	0
Sequence length	76
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.797464126511056	34.0	32.0	34.0	21.0	34.0
2	30.78877998021659	34.0	32.0	34.0	21.0	34.0
3	30.667156969798185	34.0	32.0	34.0	21.0	34.0
4	30.869795614249195	34.0	32.0	34.0	21.0	34.0
5	30.900093778502885	34.0	32.0	34.0	21.0	34.0
6	33.404834089128116	37.0	34.0	38.0	21.0	38.0
7	33.32311190473132	37.0	34.0	38.0	21.0	38.0
8	33.08783705663965	37.0	33.0	38.0	21.0	38.0
9	33.54063949231145	37.0	34.0	38.0	21.0	38.0
10-11	33.371391775753764	37.0	34.0	38.0	21.0	38.0
12-13	33.32723880117673	37.0	34.0	38.0	21.0	38.0
14-15	33.224753670850305	37.0	34.0	38.0	21.0	38.0
16-17	33.17595994501754	37.0	33.5	38.0	21.0	38.0
18-19	33.204016417661194	37.0	33.5	38.0	21.0	38.0
20-21	33.158787559575046	37.0	33.5	38.0	21.0	38.0
22-23	33.22855940803926	37.0	34.0	38.0	21.0	38.0
24-25	33.212013283146845	37.0	34.0	38.0	21.0	38.0
26-27	33.14055534858626	37.0	34.0	38.0	21.0	38.0
28-29	33.229378364143216	37.0	34.0	38.0	21.0	38.0
30-31	33.30111249566435	37.0	34.0	38.0	21.0	38.0
32-33	33.25909844173529	37.0	34.0	38.0	21.0	38.0
34-35	33.28359004663233	37.0	34.0	38.0	21.0	38.0
36-37	33.11375139704276	37.0	33.5	38.0	21.0	38.0
38-39	33.130583353673416	37.0	33.5	38.0	21.0	38.0
40-41	33.14848477062806	37.0	33.5	38.0	21.0	38.0
42-43	33.212716621918474	37.0	34.0	38.0	21.0	38.0
44-45	33.23609701578819	37.0	34.0	38.0	21.0	38.0
46-47	33.17439911103118	37.0	34.0	38.0	21.0	38.0
48-49	33.1513784155287	37.0	34.0	38.0	21.0	38.0
50-51	33.009262232956075	37.0	32.5	38.0	21.0	38.0
52-53	33.088068291304296	37.0	33.0	38.0	21.0	38.0
54-55	33.0570282491682	37.0	33.0	38.0	21.0	38.0
56-57	33.028939660598894	37.0	33.0	38.0	21.0	38.0
58-59	33.01746785195843	37.0	32.5	38.0	21.0	38.0
60-61	33.00481738884678	37.0	33.0	38.0	21.0	38.0
62-63	32.964951890343386	37.0	32.5	38.0	21.0	38.0
64-65	32.97561437765759	37.0	32.5	38.0	21.0	38.0
66-67	32.872117595673345	37.0	32.0	38.0	21.0	38.0
68-69	32.611204604139104	37.0	31.5	38.0	16.0	38.0
70-71	32.80285639042688	37.0	32.0	38.0	21.0	38.0
72-73	32.78074136402759	37.0	32.0	38.0	21.0	38.0
74-75	32.79933327338361	37.0	31.0	38.0	21.0	38.0
76	31.43332091517542	36.0	27.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	16.0
14	79.0
15	201.0
16	441.0
17	914.0
18	1324.0
19	1614.0
20	1860.0
21	1998.0
22	2166.0
23	2403.0
24	2695.0
25	3060.0
26	3605.0
27	4197.0
28	4700.0
29	5425.0
30	6152.0
31	7004.0
32	8171.0
33	9825.0
34	11927.0
35	15129.0
36	21642.0
37	39137.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.873283403774266	17.56034582428735	9.016867284148864	25.549503487789526
2	24.205773158793985	19.928574181365054	30.83642716750382	25.029225492337144
3	20.315249926133372	20.849016610356745	29.283301003301514	29.552432460208365
4	14.51447143609573	16.362421797721055	35.62748095525609	33.49562581092712
5	14.31149878601801	32.5385712266999	35.76557943553049	17.384350551751602
6	30.640520021068046	35.542052593039834	18.60604036329535	15.211387022596764
7	29.650707192682706	28.72255694153617	21.71421964723867	19.91251621854245
8	26.547024138329718	33.22649435402027	19.442981385609496	20.783500122040518
9	24.899477152730494	15.69184127024909	21.07960895648934	38.329072620531065
10-11	25.976324139614356	23.671364156057706	27.07790038924502	23.274411315082926
12-13	25.926223295607826	22.05496961833434	26.194712434001772	25.82409465205606
14-15	24.21123286615367	23.17260383078761	23.526842490654264	29.089320812404456
16-17	24.52950168929769	25.91337692534974	24.48068548231697	25.0764359030356
18-19	23.649525326618964	25.781380470947934	25.94870444355947	24.620389758873632
20-21	23.628649974949578	26.066248731420938	25.776563082101152	24.528538211528332
22-23	24.067353519263133	25.439024703570006	25.918515473452974	24.575106303713888
24-25	23.97646544968719	25.57937129863957	25.694988630962325	24.749174620710917
26-27	23.41540022866539	25.76692830440759	26.01133049856763	24.80634096835939
28-29	23.869198258032192	25.50422003262978	26.80395154349139	23.82263016584664
30-31	24.0467993268502	25.505825828912045	26.153925208432355	24.293449635805402
32-33	23.389386328892773	26.223616767082458	26.12791130865974	24.25908559536503
34-35	24.11038885962771	25.33529026373598	26.286563981347072	24.267756895289235
36-37	24.28831108770217	25.286474056755264	25.806109733694743	24.619105121847824
38-39	23.514317279652634	25.712652390067188	26.652685533702453	24.12034479657773
40-41	23.56152769035109	25.107909510167904	26.949436686664185	24.381126112816823
42-43	23.746194262811045	25.205220764872887	26.49403286101512	24.55455211130095
44-45	22.92049381447272	25.306707089911747	26.86625643924309	24.90654265637244
46-47	23.27987102244261	25.12300399522115	26.806520817543007	24.790604164793237
48-49	22.679624372133652	25.05845098467428	27.487057281964983	24.774867361227084
50-51	22.928843955140472	25.093457343627556	27.25646493583238	24.721233765399585
52-53	22.39796770422517	25.05652402913557	27.410621378929385	25.13488688770988
54-55	21.951235178500315	25.41076268900222	27.682322109887853	24.95568002260961
56-57	21.873835797695364	25.32565548604242	27.5519314517683	25.248577264493914
58-59	22.19788548745552	25.15704687640507	27.451087445242344	25.193980190897065
60-61	22.145536528653828	25.072903151214625	27.18388294387421	25.597677376257337
62-63	21.708759939878988	24.866718908572384	27.52527523348278	25.89924591806585
64-65	22.127872769548965	24.8847038269337	27.26866898757756	25.718754415939777
66-67	22.251519083283018	24.948614518967666	26.798170676875248	26.001695720874068
68-69	21.875120434721172	25.11850776563082	26.750960266176794	26.255411533471218
70-71	22.558226173194765	25.130390658119552	26.766375910486488	25.5450072581992
72-73	22.546985599218942	24.651863366005937	26.354649743714916	26.44650129106021
74-75	22.427514355818765	24.968205233611243	26.46609200570379	26.138188404866202
76	22.38736944876225	25.029225492337144	26.17897563043562	26.404429428464987
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	2.0
11	1.5
12	0.0
13	0.5
14	3.0
15	10.5
16	26.5
17	37.0
18	40.5
19	62.0
20	101.0
21	122.0
22	142.5
23	229.0
24	384.0
25	473.0
26	585.0
27	740.0
28	975.5
29	1168.0
30	1292.0
31	1615.0
32	2133.0
33	2452.0
34	2760.5
35	3491.0
36	4246.5
37	4580.0
38	5024.5
39	6034.0
40	7029.5
41	7900.0
42	8340.0
43	8441.5
44	8859.0
45	9002.5
46	8830.0
47	8724.0
48	8289.5
49	7678.5
50	7396.0
51	6971.0
52	5990.5
53	5238.0
54	5041.0
55	4739.0
56	4183.0
57	3710.5
58	3492.0
59	3242.5
60	2812.0
61	2496.5
62	2362.0
63	2262.0
64	2065.0
65	1911.0
66	1804.5
67	1755.0
68	1697.5
69	1577.0
70	1455.5
71	1397.0
72	1328.0
73	1194.5
74	1097.5
75	1065.0
76	956.5
77	800.5
78	662.0
79	571.0
80	501.5
81	375.0
82	267.0
83	216.0
84	181.0
85	121.5
86	78.5
87	60.0
88	55.5
89	38.5
90	18.5
91	9.5
92	8.0
93	8.0
94	5.5
95	2.5
96	2.0
97	1.5
98	0.5
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	155686.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.86123350847218
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.69708216408678	50.25692740516167
2	11.81718515836376	15.093200416222396
3	3.9648773422648684	7.59605873360482
4	1.6525351276867526	4.221317266806264
5	0.9142753688784285	2.919337641149493
6	0.6075053056133892	2.3277622907647446
7	0.4787624593906843	2.140205284996724
8	0.37013568289027693	1.8909857019899028
9	0.28967140400108626	1.6648895854476315
>10	1.2059583798517446	11.814806726359468
>50	0.0020116069722297654	0.07450894749688476
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	6.423185129041789E-4	0.0
15	0.0	0.0	0.0	6.423185129041789E-4	0.0
16	0.0	0.0	0.0	6.423185129041789E-4	0.0
17	0.0	0.0	0.0	6.423185129041789E-4	0.0
18	0.0	0.0	0.0	0.0019269555387125368	0.0
19	0.0	0.0	0.0	0.0025692740516167155	0.0
20	0.0	0.0	0.0	0.0025692740516167155	0.0
21	0.0	0.0	0.0	0.0038539110774250735	0.0
22	0.0	0.0	0.0	0.0038539110774250735	0.0
23	0.0	0.0	0.0	0.0038539110774250735	0.0
24	0.0	0.0	0.0	0.0044962295903292525	0.0
25	0.0	0.0	0.0	0.0057808666161376105	0.0
26	0.0	0.0	0.0	0.007707822154850147	0.0
27	0.0	0.0	0.0	0.014773325796796116	0.0
28	0.0	0.0	0.0	0.02376578497745462	0.0
29	0.0	0.0	0.0	0.037896792261346554	0.0
30	0.0	0.0	0.0	0.04817388846781342	0.0
31	0.0	0.0	0.0	0.06487416980332207	0.0
32	0.0	0.0	0.0	0.07836285857430983	0.0
33	0.0	0.0	0.0	0.10469791760338117	0.0
34	0.0	0.0	0.0	0.1432370283776319	0.0
35	0.0	0.0	0.0	0.21838829438742083	0.0
36	0.0	0.0	0.0	0.33079403414565217	0.0
37	0.0	0.0	0.0	0.49715452898783447	0.0
38	0.0	0.0	0.0	0.7200390529655846	0.0
39	0.0	0.0	0.0	0.9795357321788729	0.0
40	0.0	0.0	0.0	1.2660097889341366	0.0
41	0.0	0.0	0.0	1.5865267268733219	0.0
42	0.0	0.0	0.0	1.906401346299603	0.0
43	0.0	0.0	0.0	2.2924347725550147	0.0
44	0.0	0.0	0.0	2.7054455763524015	0.0
45	0.0	0.0	0.0	3.167272587130506	0.0
46	0.0	0.0	0.0	3.741505337666842	0.0
47	0.0	0.0	0.0	4.313168814151561	0.0
48	0.0	0.0	0.0	4.877766786994335	0.0
49	0.0	0.0	0.0	5.450072581991958	0.0
50	0.0	0.0	0.0	5.935665377747517	0.0
51	0.0	0.0	0.0	6.461081921303136	0.0
52	0.0	0.0	0.0	6.939609213416749	0.0
53	0.0	0.0	0.0	7.407859409323896	0.0
54	0.0	0.0	0.0	7.862620916460054	0.0
55	0.0	0.0	0.0	8.384825867451152	0.0
56	0.0	0.0	0.0	8.90253458885192	0.0
57	0.0	0.0	0.0	9.439512865639813	0.0
58	0.0	0.0	0.0	10.02466503089552	0.0
59	0.0	0.0	0.0	10.507688552599463	0.0
60	0.0	0.0	0.0	11.051090014516399	0.0
61	0.0	0.0	0.0	11.573937284020401	0.0
62	0.0	0.0	0.0	12.062099353827575	0.0
63	0.0	0.0	0.0	12.544480557018614	0.0
64	0.0	0.0	0.0	13.005022930770911	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAT	15	0.0022212823	70.0	37
CGCGATA	15	0.0022212823	70.0	38
ACTGATC	25	2.3845978E-4	55.999996	24
CTTCGTG	20	0.006940807	52.5	7
GAGTCGA	20	0.006940807	52.5	58
GTGCGTT	20	0.006940807	52.5	31
ATTTGCG	20	0.006940807	52.5	36
TACTGAT	35	2.0790874E-5	50.000004	23
CGCAGTA	30	5.863664E-4	46.666664	18
CTACCCG	30	5.863664E-4	46.666664	10
CATGTAA	30	5.863664E-4	46.666664	35
CGCAAAG	30	5.863664E-4	46.666664	12
CCGCAGT	30	5.863664E-4	46.666664	17
TACCCGC	35	0.0012524271	40.000004	11
CCGTGCG	40	0.0024130347	35.0	9
GCAGTAC	45	0.0042971354	31.111113	19
CACTACG	45	0.0042971354	31.111113	45
GTATAGA	45	0.0042971354	31.111113	1
GTCCAAG	80	2.4505553E-6	30.625	1
CCTATAC	60	4.964754E-4	29.166666	3
>>END_MODULE