FastQCFastQC Report
Wed 25 May 2016
SRR1781148_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781148_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141256
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGT21871.5482528175794303No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG2710.19185025768816902No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCC1530.10831398312284081No Hit
CTTATACACATCTCCGAGCCCACGAGACGAAATGCCACCTCGTATGCCGT1450.10265050688112362No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC2200.060.45454441
TATGCCG2250.059.1111143
TCTCGTA1900.058.94736538
CTCGTAT2200.058.86363639
TCGTATG2200.058.86363640
CATCTCG1950.057.43589836
ATCTCGT2000.056.00000437
CCCAACG252.3838389E-456.00000454
CCGTCTT2250.056.047
GTATGCC2500.053.19999742
ATGCCGT2500.053.19999744
GCCGTCT2400.052.546
CAGACGG200.00693948452.59
GTCTTCT2350.052.1276649
CTTGAAA2500.051.857
TGCCGTC2550.050.78431345
CGTCTTC2500.050.39999848
TCTTCTG2550.049.41176650
GCCATCT2450.047.14285734
CTGCTTG2850.044.21052654
Produced by FastQC (version 0.11.5)