FastQCFastQC Report
Wed 25 May 2016
SRR1781147_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781147_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70204
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGGT150.002216189470.032
GGTAACT150.002216189470.030
AACTTGT150.002216189470.033
GGGTAAC150.002216189470.029
AGTTGTG150.002216189470.035
GTTATTG252.3755521E-455.99999652
ACCATAG200.006925013452.553
CGTTGGT200.006925013452.542
TGGCGGC200.006925013452.546
TGTTCCG200.006925013452.537
GTTCCGT200.006925013452.538
TCCGTTG200.006925013452.540
ATTGGAT200.006925013452.555
GTCTAGG350.00124773340.0000041
ACACTGA350.00124773340.0000046
AAATCAG400.002404044835.05
GGCGTGA400.002404044835.025
GTGTAAA501.6942093E-435.01
GGATTGC400.002404044835.09
TCCTAAA450.00428122531.1111092
Produced by FastQC (version 0.11.5)