##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781147_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	70204
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.03170759500883	34.0	34.0	34.0	27.0	34.0
2	32.20233889806848	34.0	34.0	34.0	27.0	34.0
3	32.20837843997493	34.0	34.0	34.0	27.0	34.0
4	32.33755911344083	34.0	34.0	34.0	31.0	34.0
5	32.29036807019543	34.0	34.0	34.0	30.0	34.0
6	35.28477009856988	38.0	36.0	38.0	29.0	38.0
7	35.20568628568173	38.0	36.0	38.0	27.0	38.0
8	35.253974132528064	38.0	36.0	38.0	27.0	38.0
9	35.474559854139365	38.0	37.0	38.0	31.0	38.0
10-11	35.40642271095664	38.0	36.0	38.0	31.0	38.0
12-13	35.37448008660475	38.0	36.0	38.0	30.5	38.0
14-15	35.30357956811578	38.0	36.0	38.0	29.0	38.0
16-17	35.28245541564583	38.0	36.0	38.0	29.0	38.0
18-19	35.30402826049797	38.0	36.0	38.0	29.5	38.0
20-21	35.25468634265854	38.0	36.0	38.0	28.0	38.0
22-23	35.30467637171671	38.0	36.0	38.0	29.0	38.0
24-25	35.30404250470059	38.0	36.0	38.0	29.0	38.0
26-27	35.24159592046037	38.0	36.0	38.0	27.0	38.0
28-29	35.31021736653182	38.0	36.0	38.0	30.0	38.0
30-31	35.40192154293203	38.0	36.0	38.0	31.0	38.0
32-33	35.35146857728904	38.0	36.0	38.0	29.5	38.0
34-35	35.38449376103925	38.0	36.0	38.0	31.0	38.0
36-37	35.26455045296564	38.0	36.0	38.0	28.5	38.0
38-39	35.257335764343914	38.0	36.0	38.0	27.0	38.0
40-41	35.26022021537234	38.0	36.0	38.0	27.5	38.0
42-43	35.338278445672614	38.0	36.0	38.0	30.5	38.0
44-45	35.32589311150362	38.0	36.0	38.0	30.5	38.0
46-47	35.316798188137426	38.0	36.0	38.0	29.0	38.0
48-49	35.2605976867415	38.0	36.0	38.0	27.0	38.0
50-51	35.17454133667597	38.0	36.0	38.0	27.0	38.0
52-53	35.232244601447206	38.0	36.0	38.0	27.0	38.0
54-55	35.1916130135035	38.0	36.0	38.0	27.0	38.0
56-57	35.119409150475754	38.0	36.0	38.0	27.0	38.0
58-59	35.120363512050595	38.0	36.0	38.0	27.0	38.0
60-61	35.11140390860919	38.0	36.0	38.0	27.0	38.0
62-63	35.105513930830156	38.0	36.0	38.0	27.0	38.0
64-65	35.127991282548	38.0	36.0	38.0	27.0	38.0
66-67	35.02453563899493	38.0	36.0	38.0	27.0	38.0
68-69	34.76682952538317	38.0	35.5	38.0	26.0	38.0
70-71	34.981617856532395	38.0	36.0	38.0	27.0	38.0
72-73	34.9928351660874	38.0	36.0	38.0	27.0	38.0
74-75	35.01258475300553	38.0	36.0	38.0	27.0	38.0
76	33.80304541051792	37.0	34.0	38.0	24.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	10.0
14	33.0
15	110.0
16	177.0
17	287.0
18	385.0
19	379.0
20	376.0
21	358.0
22	314.0
23	386.0
24	418.0
25	540.0
26	627.0
27	731.0
28	910.0
29	1150.0
30	1409.0
31	1703.0
32	2341.0
33	3179.0
34	4497.0
35	6768.0
36	11884.0
37	31229.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.940459233092135	19.23822004444191	10.017947695288019	25.80337302717794
2	22.454560993675575	20.377756253204947	32.077944276679396	25.089738476440086
3	19.594325109680362	21.612728619451882	29.01686513588969	29.776081134978067
4	13.939376673693808	16.25833285852658	36.3070480314512	33.49524243632842
5	14.137371089966383	33.384137655974016	35.62190188593243	16.856589368127175
6	30.795966041820975	36.3070480314512	18.1813002108142	14.715685715913624
7	29.374394621389094	28.469887755683438	22.50868896359182	19.64702865933565
8	26.15235599111162	33.86844054469831	19.84502307560823	20.134180388581846
9	24.430231895618483	15.9093498945929	21.26944333656202	38.3909748732266
10-11	25.62104723377585	24.25858925417355	27.294028830266083	22.826334681784513
12-13	25.353256224716542	22.531479687767078	26.737080508233145	25.37818357928323
14-15	23.012933735969458	23.97228078172184	24.18736824112586	28.82741724118284
16-17	23.5634721668281	26.816135832716085	25.41379408580708	24.20659791464874
18-19	22.842003304655005	26.794769528801776	26.540510512221527	23.82271665432169
20-21	22.87975044157028	26.731382827189332	26.358184718819437	24.030682012420947
22-23	23.192410688849638	26.11674548458777	26.66087402427212	24.02996980229047
24-25	22.86336960856931	26.583955330180615	26.405902797561392	24.14677226368868
26-27	22.28149393196969	26.598911742920635	27.010569198336277	24.109025126773403
28-29	22.803543957609254	26.3624579796023	27.67292461967979	23.161073443108656
30-31	23.039997720927584	26.596062902398725	26.687938009230244	23.67600136744345
32-33	22.581334396900463	26.795481738932253	26.75987123240841	23.863312631758873
34-35	23.27858811463734	26.078286137542023	26.922967352287618	23.720158395533016
36-37	23.42530340151558	26.07116403623725	26.685801378838814	23.817731183408352
38-39	22.156144949005753	26.845336448065638	27.255569483220327	23.74294911970828
40-41	23.0086604751866	25.858925417355138	27.625206540937835	23.507207566520428
42-43	22.65326762007863	25.944390633012365	27.132357130647826	24.26998461626118
44-45	22.240897954532503	25.82830038174463	27.547575636715855	24.38322602700701
46-47	22.355563785539285	25.94082958235998	27.640162953677855	24.063443678422882
48-49	21.94176969973221	25.86034983761609	28.020483163352516	24.177397299299187
50-51	22.062133211782804	25.79197766509031	27.71708164776936	24.42880747535753
52-53	21.77653694946157	25.528459916813855	28.12090479174976	24.574098341974814
54-55	21.518004672098456	25.810495128482707	28.113070480314512	24.558429719104325
56-57	21.102786166030427	25.889550452965644	28.487693008945357	24.519970372058573
58-59	21.555039598883255	25.740698535695973	28.121617001880235	24.58264486354054
60-61	21.323571306478264	25.561933792946274	28.151529827360267	24.9629650732152
62-63	21.002364537633184	25.566207053729133	28.252663665887983	25.1787647427497
64-65	21.637655974018575	25.626032704689187	27.724203749074128	25.012107572218106
66-67	21.678964161586233	25.804797447438894	27.18719731069455	25.329041080280323
68-69	21.29009743034585	25.551250640989114	27.119537348299243	26.03911458036579
70-71	21.481681955444135	25.737137485043586	27.52407270241012	25.257107857102156
72-73	21.91684234516552	25.431599339069	26.887356845763776	25.76420147000171
74-75	21.683237422369096	25.851091105919892	26.5782576491368	25.887413822574214
76	22.075665204261867	25.67659962395305	26.760583442538888	25.4871517292462
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	0.5
6	0.0
7	0.0
8	0.0
9	0.0
10	0.5
11	1.5
12	2.0
13	3.5
14	3.0
15	5.0
16	13.5
17	18.0
18	23.0
19	40.5
20	65.5
21	78.0
22	103.5
23	158.0
24	241.0
25	295.0
26	358.5
27	476.5
28	600.5
29	670.0
30	795.0
31	979.5
32	1204.5
33	1370.0
34	1497.0
35	1831.5
36	2247.5
37	2456.0
38	2630.0
39	3121.5
40	3631.5
41	3957.5
42	4091.0
43	4143.0
44	4217.5
45	4121.5
46	4003.0
47	3945.5
48	3649.0
49	3256.5
50	3103.0
51	2895.0
52	2453.5
53	2054.5
54	1889.0
55	1747.5
56	1496.0
57	1228.0
58	1070.0
59	1030.5
60	896.0
61	758.5
62	716.0
63	703.0
64	660.0
65	656.0
66	645.5
67	609.0
68	612.5
69	637.0
70	646.0
71	634.0
72	617.0
73	574.0
74	537.5
75	527.0
76	484.0
77	393.0
78	300.5
79	256.0
80	245.0
81	193.0
82	132.5
83	113.0
84	100.5
85	71.0
86	44.0
87	34.0
88	28.5
89	15.5
90	6.0
91	3.5
92	3.0
93	4.0
94	4.0
95	2.5
96	2.0
97	1.5
98	0.5
99	1.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	70204.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.81254629365849
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.56756994351773	48.977266252635175
2	14.067822685215708	18.23542818073044
3	4.709786598096745	9.157597857671927
4	1.9999560449220897	5.184889749871802
5	1.076899408804202	3.4898296393367896
6	0.6900947231928969	2.683607771636944
7	0.5362519505065823	2.4329098057090763
8	0.349442869387486	1.811862571933223
9	0.2681259752532911	1.5640134465272633
>10	0.7340498011032724	6.462594723947353
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0014244202609537918	0.0
10	0.0	0.0	0.0	0.0014244202609537918	0.0
11	0.0	0.0	0.0	0.0014244202609537918	0.0
12	0.0	0.0	0.0	0.0014244202609537918	0.0
13	0.0	0.0	0.0	0.0014244202609537918	0.0
14	0.0	0.0	0.0	0.0014244202609537918	0.0
15	0.0	0.0	0.0	0.0014244202609537918	0.0
16	0.0	0.0	0.0	0.0014244202609537918	0.0
17	0.0	0.0	0.0	0.0014244202609537918	0.0
18	0.0	0.0	0.0	0.0014244202609537918	0.0
19	0.0	0.0	0.0	0.0014244202609537918	0.0
20	0.0	0.0	0.0	0.0014244202609537918	0.0
21	0.0	0.0	0.0	0.0014244202609537918	0.0
22	0.0	0.0	0.0	0.0028488405219075836	0.0
23	0.0	0.0	0.0	0.0028488405219075836	0.0
24	0.0	0.0	0.0	0.004273260782861376	0.0
25	0.0	0.0	0.0	0.004273260782861376	0.0
26	0.0	0.0	0.0	0.004273260782861376	0.0
27	0.0	0.0	0.0	0.008546521565722751	0.0
28	0.0	0.0	0.0	0.018517463392399295	0.0
29	0.0	0.0	0.0	0.029912825480029628	0.0
30	0.0	0.0	0.0	0.04415702808956755	0.0
31	0.0	0.0	0.0	0.05127912939433651	0.0014244202609537918
32	0.0	0.0	0.0	0.06267449148196684	0.0014244202609537918
33	0.0	0.0	0.0	0.08973847644008888	0.0014244202609537918
34	0.0	0.0	0.0	0.12250014244202609	0.0014244202609537918
35	0.0	0.0	0.0	0.17805253261922396	0.0014244202609537918
36	0.0	0.0	0.0	0.27776195088598943	0.0014244202609537918
37	0.0	0.0	0.0	0.44014586063472166	0.0014244202609537918
38	0.0	0.0	0.0	0.6438379579511139	0.0014244202609537918
39	0.0	0.0	0.0	0.8760184604865819	0.0014244202609537918
40	0.0	0.0	0.0	1.14950715058971	0.0014244202609537918
41	0.0	0.0	0.0	1.4415133040852373	0.0014244202609537918
42	0.0	0.0	0.0	1.799042789584639	0.0014244202609537918
43	0.0	0.0	0.0	2.1978804626517006	0.0014244202609537918
44	0.0	0.0	0.0	2.6650903082445443	0.0014244202609537918
45	0.0	0.0	0.0	3.236282832887015	0.0014244202609537918
46	0.0	0.0	0.0	3.8373881830095153	0.0014244202609537918
47	0.0	0.0	0.0	4.468406358612045	0.0014244202609537918
48	0.0	0.0	0.0	5.066662868212638	0.0014244202609537918
49	0.0	0.0	0.0	5.6350065523332	0.0014244202609537918
50	0.0	0.0	0.0	6.139251324710843	0.0014244202609537918
51	0.0	0.0	0.0	6.680531023873284	0.0014244202609537918
52	0.0	0.0	0.0	7.237479345906216	0.0014244202609537918
53	0.0	0.0	0.0	7.784456726112472	0.0014244202609537918
54	0.0	0.0	0.0	8.419748162497863	0.0014244202609537918
55	0.0	0.0	0.0	9.089225685146145	0.0014244202609537918
56	0.0	0.0	0.0	9.691755455529599	0.0014244202609537918
57	0.0	0.0	0.0	10.307105008261637	0.0014244202609537918
58	0.0	0.0	0.0	10.909634778645092	0.0014244202609537918
59	0.0	0.0	0.0	11.351205059540767	0.0014244202609537918
60	0.0	0.0	0.0	11.869694034527948	0.0014244202609537918
61	0.0	0.0	0.0	12.469374964389493	0.0014244202609537918
62	0.0	0.0	0.0	13.049114010597687	0.0014244202609537918
63	0.0	0.0	0.0	13.560480884280098	0.0014244202609537918
64	0.0	0.0	0.0	14.08324312005014	0.0014244202609537918
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGGT	15	0.0022161894	70.0	32
GGTAACT	15	0.0022161894	70.0	30
AACTTGT	15	0.0022161894	70.0	33
GGGTAAC	15	0.0022161894	70.0	29
AGTTGTG	15	0.0022161894	70.0	35
GTTATTG	25	2.3755521E-4	55.999996	52
ACCATAG	20	0.0069250134	52.5	53
CGTTGGT	20	0.0069250134	52.5	42
TGGCGGC	20	0.0069250134	52.5	46
TGTTCCG	20	0.0069250134	52.5	37
GTTCCGT	20	0.0069250134	52.5	38
TCCGTTG	20	0.0069250134	52.5	40
ATTGGAT	20	0.0069250134	52.5	55
GTCTAGG	35	0.001247733	40.000004	1
ACACTGA	35	0.001247733	40.000004	6
AAATCAG	40	0.0024040448	35.0	5
GGCGTGA	40	0.0024040448	35.0	25
GTGTAAA	50	1.6942093E-4	35.0	1
GGATTGC	40	0.0024040448	35.0	9
TCCTAAA	45	0.004281225	31.111109	2
>>END_MODULE