FastQCFastQC Report
Wed 25 May 2016
SRR1781147_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781147_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66576
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGT7611.1430545541937034No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCG730.10964912280701754No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACGAA150.002215680270.0000124
TATCGCG150.002215680270.000015
ATCGCGT150.002215680270.000016
TCGCGTG150.002215680270.000017
ATCTCGT1350.062.2222237
CGTATGC1600.061.2541
TCGTATG1600.061.2540
TCTCGTA1400.060.038
TATGCCG1650.059.3939443
CTCGTAT1600.059.062539
CGTCTTC1600.059.062548
TATCTCG1400.057.536
CCGTCTT1750.056.047
GCCGTCT1750.056.046
GTATGCC1800.054.44444742
TGCCGTC1800.054.44444745
GTCTTCT1750.054.049
TTGAAAA1700.053.52940858
CGAAAAT200.006923437652.527
GCTATCG200.006923437652.53
Produced by FastQC (version 0.11.5)