FastQCFastQC Report
Wed 25 May 2016
SRR1781146_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781146_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58627
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATATCG150.002214354570.029
GTCCTAA150.002214354570.020
ATCGGTT150.002214354570.025
AGTAGCG150.002214354570.02
CTAAACT308.28169E-658.33333223
ACCGTAT252.3722957E-455.99999611
ATCCTCG200.006919324452.534
AACGTGT200.006919324452.526
AACCGTA200.006919324452.510
GTACCTA200.006919324452.51
AATTTCG200.006919324452.547
CGGTTGG200.006919324452.552
TAGAACC200.006919324452.54
GGTCCTA200.006919324452.519
CAACCGT200.006919324452.59
ACCTGCA200.006919324452.535
ATCCGGA200.006919324452.51
AAATTTC200.006919324452.546
CACAGGT200.006919324452.515
TGGGGCG200.006919324452.556
Produced by FastQC (version 0.11.5)