##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781146_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	58627
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.11201323622222	34.0	34.0	34.0	27.0	34.0
2	32.411721561737764	34.0	34.0	34.0	31.0	34.0
3	32.44750712129224	34.0	34.0	34.0	31.0	34.0
4	32.57396762583792	34.0	34.0	34.0	31.0	34.0
5	32.58055162297235	34.0	34.0	34.0	31.0	34.0
6	35.69606154161052	38.0	37.0	38.0	31.0	38.0
7	35.60015010148908	38.0	37.0	38.0	31.0	38.0
8	35.416565746157914	38.0	36.0	38.0	31.0	38.0
9	35.70834257253484	38.0	37.0	38.0	31.0	38.0
10-11	35.68469305951183	38.0	37.0	38.0	31.0	38.0
12-13	35.658135330137995	38.0	37.0	38.0	31.0	38.0
14-15	35.65789653231447	38.0	37.0	38.0	31.0	38.0
16-17	35.63868183601413	38.0	37.0	38.0	31.0	38.0
18-19	35.645751957289306	38.0	37.0	38.0	31.0	38.0
20-21	35.61469118324322	38.0	37.0	38.0	31.0	38.0
22-23	35.635432479915394	38.0	37.0	38.0	31.0	38.0
24-25	35.648429904310305	38.0	37.0	38.0	31.0	38.0
26-27	35.595212103638254	38.0	37.0	38.0	31.0	38.0
28-29	35.652685622665324	38.0	37.0	38.0	31.0	38.0
30-31	35.71335732682894	38.0	37.0	38.0	31.0	38.0
32-33	35.69476521056851	38.0	37.0	38.0	31.0	38.0
34-35	35.71387756494448	38.0	37.0	38.0	31.0	38.0
36-37	35.61811963770958	38.0	37.0	38.0	31.0	38.0
38-39	35.62466952086922	38.0	37.0	38.0	31.0	38.0
40-41	35.60919883330206	38.0	37.0	38.0	31.0	38.0
42-43	35.68335408600133	38.0	37.0	38.0	31.0	38.0
44-45	35.663243897862756	38.0	37.0	38.0	31.0	38.0
46-47	35.65737629419892	38.0	37.0	38.0	31.0	38.0
48-49	35.60777457485459	38.0	37.0	38.0	31.0	38.0
50-51	35.55000255854811	38.0	37.0	38.0	31.0	38.0
52-53	35.60670851314241	38.0	37.0	38.0	31.0	38.0
54-55	35.58726354751224	38.0	37.0	38.0	31.0	38.0
56-57	35.53390076244733	38.0	37.0	38.0	31.0	38.0
58-59	35.530224981663736	38.0	37.0	38.0	31.0	38.0
60-61	35.53584525901036	38.0	37.0	38.0	31.0	38.0
62-63	35.51144523854197	38.0	37.0	38.0	31.0	38.0
64-65	35.50569703378989	38.0	37.0	38.0	31.0	38.0
66-67	35.41939720606547	38.0	37.0	38.0	31.0	38.0
68-69	35.16153819912327	38.0	36.0	38.0	27.5	38.0
70-71	35.370639807597186	38.0	36.5	38.0	30.0	38.0
72-73	35.382801439609736	38.0	36.5	38.0	30.0	38.0
74-75	35.41526088662221	38.0	37.0	38.0	31.0	38.0
76	34.28930356320467	37.0	34.0	38.0	25.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	7.0
14	49.0
15	112.0
16	217.0
17	295.0
18	311.0
19	334.0
20	292.0
21	255.0
22	236.0
23	259.0
24	279.0
25	304.0
26	406.0
27	467.0
28	542.0
29	707.0
30	900.0
31	1153.0
32	1543.0
33	2067.0
34	3049.0
35	4910.0
36	9687.0
37	30245.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.789943200231974	18.975898476811025	9.198833302062189	27.035325020894806
2	22.44699541167039	19.462022617565285	32.69653913725758	25.394442833506748
3	19.443259931430912	21.882409128899656	28.89283094819793	29.781499991471506
4	13.923618810445697	15.828884302454501	36.7305166561482	33.51698023095161
5	13.444317464649394	33.834240196496495	35.97318641581524	16.748255923038872
6	30.404080031384854	37.00172275572689	17.788732154126937	14.805465058761321
7	29.222030804919235	28.921827826769235	22.054684701588005	19.801456666723524
8	25.558189912497653	33.76942364439593	19.86797891756358	20.80440752554284
9	24.637112593173793	14.849813225988026	21.072202227642553	39.440871953195625
10-11	25.061831579306464	23.94289320620192	28.248929674040973	22.746345540450648
12-13	24.832415098845242	22.632916574274653	26.992682552407594	25.541985774472515
14-15	23.224794036877206	23.579579374690844	24.050352226789702	29.145274361642247
16-17	23.555699592338	27.00973953980248	24.968870998004334	24.465689869855186
18-19	22.699438825114708	26.78032305934126	26.422126324048644	24.098111791495384
20-21	22.686646084568544	26.656659900728336	26.48012008119126	24.176573933511865
22-23	22.878537192761016	25.95902911627748	27.04300066522251	24.119433025738992
24-25	22.914356866290277	26.281406178040832	26.521909700308733	24.28232725536016
26-27	22.278131236461014	26.208061132242822	27.076261790642537	24.437545840653623
28-29	22.673000494652634	26.23620516144439	27.484776638750063	23.606017705152915
30-31	22.799222201374793	26.51593975472052	26.765824620055607	23.91901342384908
32-33	22.49646067511556	26.727446398417108	27.006328142323504	23.769764784143824
34-35	23.165094580995106	25.970969007453903	27.106964367953335	23.75697204359766
36-37	23.252085216709027	25.977791802411858	26.623398775308303	24.146724205570813
38-39	22.386443106418543	26.830641172156174	27.223804731608304	23.55911098981698
40-41	22.657649205997238	25.922356593378478	27.545328944001913	23.874665256622375
42-43	22.601361147594112	26.121923345898644	27.070291845054328	24.206423661452913
44-45	22.074300237092125	26.306991659133168	27.419960086649496	24.198748017125215
46-47	22.33868354171286	26.154331621948934	27.377317618162277	24.129667218175925
48-49	21.940402886042268	26.24132225766285	27.60417555051427	24.21409930578061
50-51	22.168966517133747	25.980350350521093	27.26900574820475	24.581677384140413
52-53	22.162143722175788	25.85583434253842	27.544476094632163	24.437545840653623
54-55	21.507155406212156	26.571374963753904	27.49245228307776	24.42901734695618
56-57	21.808211233731896	26.13812748392379	27.627202483497364	24.426458798846948
58-59	21.93699148856329	25.903593907244105	27.53338905282549	24.626025551367118
60-61	21.400549234994116	26.09633786480632	27.64425947089225	24.858853429307317
62-63	21.02017841608815	25.868627083084583	28.13464785849523	24.976546642332032
64-65	21.792007095706754	26.32404864652805	27.297149777406315	24.58679448035888
66-67	21.620584372388148	26.091220768587853	27.39949170177563	24.88870315724837
68-69	21.143841574701074	26.16627151312535	27.244273116482166	25.445613795691408
70-71	21.9352857898238	26.246439353881318	27.028502225936858	24.789772630358026
72-73	21.878144882050933	25.713408497791118	27.11122861480205	25.297218005355894
74-75	21.97878110768076	26.133863237075065	26.996946799256317	24.890408855987857
76	21.89776041755505	25.756050966278337	26.989271154928616	25.356917461237998
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.5
11	1.0
12	1.0
13	1.0
14	2.5
15	7.0
16	12.5
17	15.0
18	22.0
19	36.5
20	56.5
21	69.0
22	76.5
23	118.5
24	176.0
25	199.0
26	255.0
27	329.5
28	436.5
29	525.0
30	609.0
31	715.0
32	959.5
33	1182.0
34	1282.0
35	1558.5
36	1862.0
37	1989.0
38	2126.0
39	2530.5
40	3039.0
41	3376.5
42	3473.0
43	3521.5
44	3562.0
45	3520.0
46	3486.0
47	3451.5
48	3174.5
49	2821.0
50	2710.0
51	2481.5
52	2041.0
53	1714.5
54	1600.0
55	1514.5
56	1346.5
57	1138.5
58	1013.0
59	888.0
60	702.0
61	623.5
62	606.0
63	586.5
64	536.0
65	501.5
66	517.0
67	536.0
68	513.0
69	503.5
70	503.5
71	490.0
72	495.0
73	470.5
74	404.0
75	367.0
76	360.0
77	308.0
78	232.5
79	202.0
80	189.5
81	143.0
82	99.0
83	89.0
84	70.5
85	43.5
86	23.0
87	11.0
88	9.5
89	9.0
90	8.0
91	6.0
92	6.0
93	4.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	58627.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.774830709400106
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.37429013939081	47.31608303341464
2	13.539657093171753	16.998993637743702
3	4.556693747792299	8.581370358367305
4	2.219927723283428	5.574223480648848
5	1.2852213134798793	4.033977518890613
6	0.8260196179659267	3.111194500827264
7	0.5407167893921692	2.3760383441076636
8	0.41844414857484447	2.101420847049994
9	0.3043230171453414	1.7193443294045405
>10	0.9319892400076082	8.100363313831512
>50	0.002717169795940548	0.08699063571392021
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0017056987394886314	0.0
15	0.0	0.0	0.0	0.0017056987394886314	0.0
16	0.0	0.0	0.0	0.0017056987394886314	0.0
17	0.0	0.0	0.0	0.0017056987394886314	0.0
18	0.0	0.0	0.0	0.0017056987394886314	0.0
19	0.0	0.0	0.0	0.0017056987394886314	0.0
20	0.0	0.0	0.0	0.003411397478977263	0.0
21	0.0	0.0	0.0	0.003411397478977263	0.0
22	0.0	0.0	0.0	0.0051170962184658945	0.0
23	0.0	0.0	0.0	0.0051170962184658945	0.0
24	0.0	0.0	0.0	0.013645589915909051	0.0
25	0.0	0.0	0.0	0.013645589915909051	0.0
26	0.0	0.0	0.0	0.015351288655397683	0.0
27	0.0	0.0	0.0	0.015351288655397683	0.0
28	0.0	0.0	0.0	0.023879782352840842	0.0
29	0.0	0.0	0.0	0.03411397478977263	0.0
30	0.0	0.0	0.0	0.03581967352926126	0.0
31	0.0	0.0	0.0	0.04434816722670442	0.0
32	0.0	0.0017056987394886314	0.0	0.05799375714261347	0.0
33	0.0	0.0017056987394886314	0.0	0.07163934705852253	0.0
34	0.0	0.0017056987394886314	0.0	0.09551912941136337	0.0
35	0.0	0.0017056987394886314	0.0	0.1398672966380678	0.0
36	0.0	0.0017056987394886314	0.0	0.19274395756221535	0.0
37	0.0	0.0017056987394886314	0.0	0.28996878571306733	0.0
38	0.0	0.0017056987394886314	0.0	0.3991335050403398	0.0
39	0.0	0.0017056987394886314	0.0	0.5219438142835212	0.0
40	0.0	0.0017056987394886314	0.0	0.694219386971873	0.0
41	0.0	0.0017056987394886314	0.0	0.8630835621812476	0.0
42	0.0	0.0017056987394886314	0.0	1.0814130008357923	0.0
43	0.0	0.0017056987394886314	0.0	1.304859535708803	0.0
44	0.0	0.0017056987394886314	0.0	1.5862998277244273	0.0
45	0.0	0.0017056987394886314	0.0	1.9240281781431763	0.0
46	0.0	0.0017056987394886314	0.0	2.300987599570164	0.0
47	0.0	0.0017056987394886314	0.0	2.6643014310812423	0.0
48	0.0	0.0017056987394886314	0.0	3.013969672676412	0.0
49	0.0	0.0017056987394886314	0.0	3.4045746840193085	0.0
50	0.0	0.0017056987394886314	0.0	3.7798284067068075	0.0
51	0.0	0.0017056987394886314	0.0	4.132908045780955	0.0
52	0.0	0.0017056987394886314	0.0	4.506456069728965	0.0
53	0.0	0.0017056987394886314	0.0	4.891943984853395	0.0
54	0.0	0.0017056987394886314	0.0	5.304723079809644	0.0
55	0.0	0.0017056987394886314	0.0	5.765261739471574	0.0
56	0.0	0.0017056987394886314	0.0	6.2684428676207204	0.0
57	0.0	0.0017056987394886314	0.0	6.684633360055947	0.0
58	0.0	0.0017056987394886314	0.0	7.158817609633786	0.0
59	0.0	0.0017056987394886314	0.0	7.557951114674126	0.0
60	0.0	0.0017056987394886314	0.0	7.9741416071093525	0.0
61	0.0	0.0017056987394886314	0.0	8.347689631057362	0.0
62	0.0	0.0017056987394886314	0.0	8.755351629795145	0.0
63	0.0	0.0017056987394886314	0.0	9.142545243659065	0.0
64	0.0	0.0017056987394886314	0.0	9.548501543657359	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATATCG	15	0.0022143545	70.0	29
GTCCTAA	15	0.0022143545	70.0	20
ATCGGTT	15	0.0022143545	70.0	25
AGTAGCG	15	0.0022143545	70.0	2
CTAAACT	30	8.28169E-6	58.333332	23
ACCGTAT	25	2.3722957E-4	55.999996	11
ATCCTCG	20	0.0069193244	52.5	34
AACGTGT	20	0.0069193244	52.5	26
AACCGTA	20	0.0069193244	52.5	10
GTACCTA	20	0.0069193244	52.5	1
AATTTCG	20	0.0069193244	52.5	47
CGGTTGG	20	0.0069193244	52.5	52
TAGAACC	20	0.0069193244	52.5	4
GGTCCTA	20	0.0069193244	52.5	19
CAACCGT	20	0.0069193244	52.5	9
ACCTGCA	20	0.0069193244	52.5	35
ATCCGGA	20	0.0069193244	52.5	1
AAATTTC	20	0.0069193244	52.5	46
CACAGGT	20	0.0069193244	52.5	15
TGGGGCG	20	0.0069193244	52.5	56
>>END_MODULE