FastQCFastQC Report
Wed 25 May 2016
SRR1781146_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781146_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55732
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT5641.0119859326778153No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACT150.002213776370.034
GTTCTGC150.002213776370.024
CATGTAC150.002213776370.03
GGACCGT150.002213776370.06
GCTCGGA207.862675E-570.011
CCGTCTT1200.058.33333247
TATGCCG1200.055.41666443
ATGCCGT1200.055.41666444
GCCGTCT1200.055.41666446
CGTCTTC1300.053.84615348
CGTATGC1250.053.19999741
GTATGCC1250.053.19999742
CTTGAAA1250.053.19999757
TCGCCCC200.006917531652.538
CGAAATT200.006917531652.548
CCAGGAC200.006917531652.53
ATCAAAT200.006917531652.528
AATGCAG200.006917531652.558
GTAGCCC200.006917531652.59
GTTCGTA200.006917531652.525
Produced by FastQC (version 0.11.5)