##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781146_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	55732
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.83666475274528	34.0	34.0	34.0	31.0	34.0
2	32.87603172324697	34.0	34.0	34.0	31.0	34.0
3	32.99694968779158	34.0	34.0	34.0	31.0	34.0
4	33.13633101270365	34.0	34.0	34.0	31.0	34.0
5	33.10198808583937	34.0	34.0	34.0	31.0	34.0
6	36.12665972870165	38.0	36.0	38.0	32.0	38.0
7	36.00008971506495	38.0	36.0	38.0	32.0	38.0
8	35.6313966841312	38.0	36.0	38.0	31.0	38.0
9	35.72491566783894	38.0	36.0	38.0	31.0	38.0
10-11	35.63607981052178	38.0	36.0	38.0	31.0	38.0
12-13	35.55784827388215	38.0	36.0	38.0	31.0	38.0
14-15	35.60268786334601	38.0	36.0	38.0	31.0	38.0
16-17	35.47417103279983	38.0	36.0	38.0	31.0	38.0
18-19	35.50216213306538	38.0	36.0	38.0	31.0	38.0
20-21	35.3742284504414	37.0	35.5	38.0	31.0	38.0
22-23	35.48144692456758	37.0	36.0	38.0	31.0	38.0
24-25	35.472870164358	37.0	36.0	38.0	31.0	38.0
26-27	35.21213127108304	37.0	35.5	38.0	29.0	38.0
28-29	35.174890547620755	37.0	35.0	38.0	27.0	38.0
30-31	35.22960776573602	37.0	35.0	38.0	30.0	38.0
32-33	35.16520132060576	37.0	35.0	38.0	28.0	38.0
34-35	35.16582932606043	37.0	35.0	38.0	28.5	38.0
36-37	35.11763439316731	37.0	35.0	38.0	27.0	38.0
38-39	35.025039474628585	37.0	35.0	38.0	27.0	38.0
40-41	35.04659800473695	37.0	35.0	38.0	27.0	38.0
42-43	35.094757051604105	37.0	35.0	38.0	27.0	38.0
44-45	35.01857998995192	37.0	35.0	38.0	27.0	38.0
46-47	35.03677420512452	37.0	35.0	38.0	27.0	38.0
48-49	35.03096964042202	37.0	35.0	38.0	27.0	38.0
50-51	34.89970752888825	37.0	35.0	38.0	27.0	38.0
52-53	34.931206488193496	37.0	35.0	38.0	27.0	38.0
54-55	34.95379674154884	37.0	35.0	38.0	27.0	38.0
56-57	34.878579631091654	37.0	35.0	38.0	27.0	38.0
58-59	34.86270903610134	37.0	35.0	38.0	27.0	38.0
60-61	34.83865642718725	37.0	34.5	38.0	27.0	38.0
62-63	34.830474413263474	37.0	35.0	38.0	27.0	38.0
64-65	34.76535024761358	37.0	34.0	38.0	27.0	38.0
66-67	34.741575755400845	37.0	34.0	38.0	27.0	38.0
68-69	34.649743414914234	37.0	34.0	38.0	27.0	38.0
70-71	34.59721524438384	37.0	34.0	38.0	26.5	38.0
72-73	34.50226081963683	37.0	34.0	38.0	25.0	38.0
74-75	34.51650757195148	37.0	34.0	38.0	25.5	38.0
76	33.506351826598724	37.0	34.0	38.0	23.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	8.0
22	11.0
23	51.0
24	72.0
25	163.0
26	296.0
27	576.0
28	887.0
29	1302.0
30	1880.0
31	2680.0
32	3697.0
33	4729.0
34	5978.0
35	7614.0
36	10450.0
37	15337.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.16435799899519	19.35333381181368	9.502619679896648	25.97968850929448
2	23.4317806646092	21.050742840737815	31.339266489628937	24.17821000502404
3	20.695471183521136	23.029857173616595	29.42474700351683	26.849924639345442
4	14.449508361444055	17.84432641929233	35.97574104643652	31.7304241728271
5	14.133711332807005	34.909926074786476	35.5469030359578	15.409459556448718
6	30.91401708174837	38.451876839158835	16.993827603531184	13.640278475561615
7	29.614942941218693	29.09818416708534	21.725400129189694	19.56147276250628
8	26.193210363884305	35.67250412689299	18.542309624632168	19.59197588459054
9	25.28708820785186	15.300007177205197	20.175123806789635	39.2377808081533
10-11	25.264659441613436	24.971291179214813	27.874470681116776	21.889578698054976
12-13	25.61993109883012	22.581281848848057	26.921696691308405	24.877090361013423
14-15	23.538541591904114	23.395894638627716	23.70451446206847	29.3610493073997
16-17	24.363920189478215	27.773092657719083	24.338799971291177	23.524187181511518
18-19	24.159369841383764	26.620254073063947	25.629799755975025	23.59057632957726
20-21	23.115983635972153	26.622945525012558	25.78590396899447	24.475166870020814
22-23	23.43716356850642	26.69561472762506	26.13758702361301	23.72963468025551
24-25	23.779875116629583	27.1199669848561	26.008397330079667	23.09176056843465
26-27	23.212875906122157	27.58738247326491	26.124129763869945	23.075611856742984
28-29	23.298105217828176	26.892987870523218	26.648065743199602	23.160841168449007
30-31	23.691057202325414	27.76232684992464	25.986865714490776	22.55975023325917
32-33	22.93296490346659	27.685171894064453	26.573602239288018	22.80826096318094
34-35	23.414734802267997	26.936948252350533	26.37802339768894	23.27029354769253
36-37	24.403394818057848	26.749443766597285	25.699777506638917	23.147383908705947
38-39	22.82261537357353	26.852616091294053	27.01500035886026	23.30976817627216
40-41	23.093554869733726	25.51137587023613	27.81525873824733	23.579810521782818
42-43	23.94315653484533	25.737457833919468	27.29401421086629	23.02537142036891
44-45	22.61447642288093	27.153161558888968	27.187253283571376	23.045108734658722
46-47	23.552896002296706	25.88907629369124	26.641785688652835	23.916242015359217
48-49	23.373465872389293	25.84511591186392	27.06075504198665	23.720663173760137
50-51	22.65395105146056	25.89176774563985	27.400775138161197	24.053506064738393
52-53	22.496052537142035	25.427940859829185	28.777004234551068	23.299002368477716
54-55	22.352508433216105	25.492535706595852	28.012631881145484	24.14232397904256
56-57	22.857604248905478	25.513170171535204	27.30298571736166	24.32623986219766
58-59	22.29867939424388	25.46562118710974	28.454927151367258	23.780772267279122
60-61	22.646773846264264	26.67498026268571	26.909136582214888	23.76910930883514
62-63	21.080348812172538	27.091258164070908	27.467164286226943	24.361228737529604
64-65	21.78730352400775	27.58738247326491	26.636402784755614	23.988911217971722
66-67	21.36205411612718	27.62775425249408	26.61576831981626	24.394423311562477
68-69	21.09290892126606	28.026089140888537	26.204873322328286	24.67612861551712
70-71	21.652730926577192	27.154058709538504	26.521567501614875	24.671642862269433
72-73	21.763977607119788	26.819421517261176	26.064020670350967	25.352580205268065
74-75	21.419471757697554	26.807758558817195	26.43364673796024	25.339122945525013
76	21.70207421230173	26.91451948611211	26.401349314576905	24.98205698700926
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	2.0
14	3.0
15	4.5
16	12.5
17	18.0
18	18.0
19	24.5
20	38.0
21	45.0
22	67.0
23	116.5
24	191.5
25	239.0
26	244.0
27	313.0
28	422.0
29	467.0
30	560.0
31	690.0
32	908.5
33	1090.0
34	1171.5
35	1401.0
36	1709.5
37	1870.0
38	2067.0
39	2549.0
40	3011.5
41	3295.5
42	3402.0
43	3473.0
44	3499.5
45	3358.5
46	3262.0
47	3245.0
48	3014.5
49	2671.0
50	2541.0
51	2335.0
52	1908.5
53	1578.5
54	1469.0
55	1393.5
56	1188.5
57	989.5
58	920.0
59	800.0
60	666.5
61	602.5
62	552.0
63	530.5
64	506.0
65	523.0
66	513.5
67	484.0
68	499.0
69	481.5
70	467.0
71	485.0
72	482.0
73	441.5
74	380.5
75	357.0
76	334.5
77	281.5
78	228.5
79	206.0
80	165.0
81	110.5
82	94.0
83	91.0
84	69.0
85	38.0
86	19.5
87	10.0
88	10.0
89	6.0
90	3.0
91	3.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	55732.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.16478863130697
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.7492259182456	47.84683844111103
2	13.638042212311452	17.228881073709896
3	4.46836917308184	8.467307830330869
4	2.06232423373008	5.210650972511304
5	1.224327472090447	3.8667192995047728
6	0.8181120927193706	3.100552644800115
7	0.5368860608470869	2.3738606186750877
8	0.36928670851916034	1.866073351037106
9	0.27270403090645684	1.5502763224000575
>10	0.8578814305598955	7.476853513241943
>50	0.0	0.0
>100	0.0	0.0
>500	0.002840666988608925	1.0119859326778153
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT	564	1.0119859326778153	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0017943012990741406	0.0
20	0.0	0.0	0.0	0.003588602598148281	0.0
21	0.0	0.0	0.0	0.003588602598148281	0.0
22	0.0	0.0	0.0	0.003588602598148281	0.0
23	0.0	0.0	0.0	0.003588602598148281	0.0
24	0.0	0.0	0.0	0.005382903897222422	0.0
25	0.0	0.0	0.0	0.007177205196296562	0.0
26	0.0	0.0	0.0	0.008971506495370703	0.0
27	0.0	0.0	0.0	0.008971506495370703	0.0
28	0.0	0.0	0.0	0.016148711691667265	0.0
29	0.0	0.0	0.0	0.025120218187037968	0.0
30	0.0	0.0	0.0	0.025120218187037968	0.0
31	0.0	0.0	0.0	0.035886025981482814	0.0
32	0.0	0.0	0.0	0.04665183377592765	0.0
33	0.0	0.0	0.0	0.061006244168520775	0.0
34	0.0	0.0	0.0	0.08433216105648461	0.0
35	0.0	0.0	0.0	0.1238067896361157	0.0
36	0.0	0.0	0.0	0.17404722601019162	0.0
37	0.0	0.0	0.0	0.28170530395464005	0.0
38	0.0	0.0	0.0	0.39654058709538503	0.0
39	0.0	0.0	0.0	0.5203473767315008	0.0
40	0.0	0.0	0.0	0.6997775066389148	0.0
41	0.0	0.0	0.0	0.8612646235555874	0.0
42	0.0	0.0	0.0	1.072992176846336	0.0
43	0.0	0.0	0.0	1.2918969353333811	0.0
44	0.0	0.0	0.0	1.5736022392880213	0.0
45	0.0	0.0	0.0	1.8965764731213666	0.0
46	0.0	0.0	0.0	2.2662025407306396	0.0
47	0.0	0.0	0.0	2.6322400057417643	0.0
48	0.0	0.0	0.0	2.974951553864925	0.0
49	0.0	0.0	0.0	3.344577621474198	0.0
50	0.0	0.0	0.0	3.701643579989952	0.0
51	0.0	0.0	0.0	4.051532333309409	0.0
52	0.0	0.0	0.0	4.413981195722386	0.0
53	0.0	0.0	0.0	4.781812962032585	0.0
54	0.0	0.0	0.0	5.190913658221489	0.0
55	0.0	0.0	0.0	5.6502547907844685	0.0
56	0.0	0.0	0.0	6.140099045431709	0.0
57	0.0	0.0	0.0	6.56534845331228	0.0
58	0.0	0.0	0.0	7.02468958587526	0.0
59	0.0	0.0	0.0	7.439173185961387	0.0
60	0.0	0.0	0.0	7.8410966769539945	0.0
61	0.0	0.0	0.0	8.20354553936697	0.0
62	0.0	0.0	0.0	8.605469030359577	0.0
63	0.0	0.0	0.0	8.976889399267925	0.0
64	0.0	0.0	0.0	9.389578698054978	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCACT	15	0.0022137763	70.0	34
GTTCTGC	15	0.0022137763	70.0	24
CATGTAC	15	0.0022137763	70.0	3
GGACCGT	15	0.0022137763	70.0	6
GCTCGGA	20	7.862675E-5	70.0	11
CCGTCTT	120	0.0	58.333332	47
TATGCCG	120	0.0	55.416664	43
ATGCCGT	120	0.0	55.416664	44
GCCGTCT	120	0.0	55.416664	46
CGTCTTC	130	0.0	53.846153	48
CGTATGC	125	0.0	53.199997	41
GTATGCC	125	0.0	53.199997	42
CTTGAAA	125	0.0	53.199997	57
TCGCCCC	20	0.0069175316	52.5	38
CGAAATT	20	0.0069175316	52.5	48
CCAGGAC	20	0.0069175316	52.5	3
ATCAAAT	20	0.0069175316	52.5	28
AATGCAG	20	0.0069175316	52.5	58
GTAGCCC	20	0.0069175316	52.5	9
GTTCGTA	20	0.0069175316	52.5	25
>>END_MODULE