FastQCFastQC Report
Wed 25 May 2016
SRR1781145_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781145_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60300
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGTC150.002214667270.058
TCCGTGA200.006920295352.511
CCGCGTT200.006920295352.553
ATGATGA400.002401361235.026
CATATTG450.004276473531.11111327
TCGTAGT450.004276473531.11111357
CTGGTTC500.00715716128.00000237
CAGTGGT500.00715716128.00000249
CCTTCTT1000.00958743417.5000028
CGACGAG1401.8289682E-417.569
GACGAGA1200.001321202617.570
CCGACGA1553.976255E-415.80645268
TGCCGAC2153.157251E-413.02325668
CTGCCGA2407.9746224E-411.66666667
GCCGACG2200.00556409111.13636367
GCTGCCG2850.0033408829.82456166
CGCTGCC3150.0076216858.88888870
TGACGCT4250.00110572828.23529470
GACGCTG3950.0052917377.97468370
TCTGACG4800.00366037787.291666568
Produced by FastQC (version 0.11.5)