FastQCFastQC Report
Wed 25 May 2016
SRR1781145_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781145_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56785
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGT7031.238002993748349No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCG640.1127058201989962No Hit
CTTATACACATCTCCGAGCCCACGAGACAGACCAGCACCTCGTATGCCGT610.10742273487716826No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTAT150.002213992270.039
ATATCGT150.002213992270.037
TCTATAC301.0068288E-770.03
GCATATC150.002213992270.035
CAGCATA252.3716534E-456.00000433
TCCATAC200.006918202652.52
ACAAAGT200.006918202652.52
CATATCG200.006918202652.536
CGTCTTC1400.052.548
CTCTTGC200.006918202652.51
CATTCCC200.006918202652.525
CCGTCTT1400.052.547
ACCTAAG200.006918202652.538
CGCTCAT200.006918202652.565
CTAAACA200.006918202652.54
ATAACAT200.006918202652.536
GTAAGAC200.006918202652.53
TATCGTA200.006918202652.538
GCCGTCT1400.052.546
CGTATGC1550.051.93548241
Produced by FastQC (version 0.11.5)