##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781145_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	56785
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.990420005283084	34.0	34.0	34.0	31.0	34.0
2	33.01569076340583	34.0	34.0	34.0	31.0	34.0
3	33.08889671568196	34.0	34.0	34.0	31.0	34.0
4	33.20179624900942	34.0	34.0	34.0	32.0	34.0
5	33.16116932288457	34.0	34.0	34.0	32.0	34.0
6	36.31853482433741	38.0	37.0	38.0	34.0	38.0
7	36.22220656863608	38.0	37.0	38.0	34.0	38.0
8	35.9150303777406	38.0	36.0	38.0	31.0	38.0
9	35.92839658360482	38.0	36.0	38.0	32.0	38.0
10-11	35.82287575944352	38.0	36.0	38.0	31.0	38.0
12-13	35.75055912652989	38.0	36.0	38.0	31.0	38.0
14-15	35.787153297525755	38.0	36.0	38.0	31.0	38.0
16-17	35.69770185788501	38.0	36.0	38.0	31.0	38.0
18-19	35.71436118693317	38.0	36.0	38.0	31.0	38.0
20-21	35.619670687681605	38.0	36.0	38.0	31.0	38.0
22-23	35.70061635995421	38.0	36.0	38.0	31.0	38.0
24-25	35.697103108215195	38.0	36.0	38.0	31.0	38.0
26-27	35.421475741833234	38.0	36.0	38.0	31.0	38.0
28-29	35.379184643832	38.0	36.0	38.0	31.0	38.0
30-31	35.456837192920666	38.0	36.0	38.0	31.0	38.0
32-33	35.35117548648411	38.0	36.0	38.0	31.0	38.0
34-35	35.33861935370256	38.0	36.0	38.0	31.0	38.0
36-37	35.28536585365853	38.0	36.0	38.0	30.5	38.0
38-39	35.19166153033372	38.0	36.0	38.0	27.5	38.0
40-41	35.27871797129524	38.0	36.0	38.0	30.5	38.0
42-43	35.279589680373334	38.0	36.0	38.0	30.5	38.0
44-45	35.237069648674826	38.0	36.0	38.0	29.0	38.0
46-47	35.27187637580347	38.0	36.0	38.0	30.0	38.0
48-49	35.26157435942591	38.0	36.0	38.0	30.0	38.0
50-51	35.134445716298316	37.0	35.0	38.0	27.0	38.0
52-53	35.17103988729418	37.5	35.0	38.0	27.0	38.0
54-55	35.21021396495553	37.5	35.5	38.0	28.0	38.0
56-57	35.10068680109184	37.0	35.0	38.0	27.0	38.0
58-59	35.11392092982302	37.0	35.0	38.0	27.0	38.0
60-61	35.1190983534384	37.0	35.0	38.0	27.0	38.0
62-63	35.05498811305802	37.0	35.0	38.0	27.0	38.0
64-65	34.97869155586862	37.0	35.0	38.0	27.0	38.0
66-67	34.9676234921194	37.0	35.0	38.0	27.0	38.0
68-69	34.87724751254733	37.0	35.0	38.0	27.0	38.0
70-71	34.77206128378973	37.0	35.0	38.0	27.0	38.0
72-73	34.670476358193184	37.0	35.0	38.0	27.0	38.0
74-75	34.70354847230783	37.0	35.0	38.0	27.0	38.0
76	33.68190543277274	37.0	34.0	38.0	24.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	2.0
21	6.0
22	18.0
23	32.0
24	73.0
25	137.0
26	309.0
27	503.0
28	769.0
29	1192.0
30	1790.0
31	2489.0
32	3355.0
33	4521.0
34	5715.0
35	7421.0
36	10347.0
37	18106.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	44.906225235537555	19.24804085585982	10.145284846350268	25.700449062252357
2	23.19626661970591	21.070705291890466	32.23210354847231	23.50092453993132
3	20.968565642335125	22.766575680197235	29.941005547239584	26.323853130228052
4	15.229373954389363	18.689794840186668	35.96724487100467	30.113586334419303
5	14.914149863520295	34.102315752399406	35.32799154706348	15.655542837016817
6	31.163159285022452	36.93404948489918	18.032931231839395	13.86985999823897
7	29.231311085674033	29.071057497578582	21.822664436030642	19.87496698071674
8	25.991018754952893	35.63617152417011	19.253323941181648	19.119485779695342
9	25.006603856652287	16.22435502333363	21.093598661618383	37.6754424583957
10-11	25.866866249889934	25.178304129611696	27.153297525755043	21.80153209474333
12-13	25.858941621907196	22.657391916879458	26.23668222241789	25.246984238795456
14-15	23.92797393677908	23.516773795896803	23.448974200933346	29.10627806639077
16-17	25.43277273927974	26.997446508761115	24.45011886941974	23.119661882539404
18-19	24.64647354054768	25.830765166857443	25.732147574183323	23.790613718411553
20-21	23.526459452320157	26.103724575151887	25.405476798450294	24.96433917407766
22-23	24.17011534736286	26.424231751342788	25.699568548032055	23.706084353262305
24-25	24.573390860262393	26.794928238091043	25.76736814299551	22.86431275865105
26-27	24.112881923043055	26.949898740864665	25.917936074667608	23.019283261424672
28-29	23.74042440785419	26.48146517566259	26.5589504270494	23.21915998943383
30-31	24.797041472219778	26.583604825217925	26.000704411376248	22.618649291186053
32-33	23.569604649115085	26.318570044906224	25.68900237738839	24.422822928590296
34-35	25.099938364004583	26.051774236153914	26.012151096240206	22.836136303601304
36-37	24.369992075372018	26.021836752663557	25.585101699392442	24.023069472571983
38-39	23.561680021132343	26.103724575151887	26.96222593994893	23.37236946376684
40-41	23.622435502333364	25.175662586950782	27.512547327639343	23.689354583076515
42-43	24.607730914854276	25.19327287135687	26.928766399577352	23.2702298142115
44-45	22.944439552698775	26.32209210178744	27.49845910011447	23.23500924539931
46-47	24.142819406533416	24.898300607554813	26.915558686272785	24.04332129963899
48-49	23.69992075372017	24.821695870388307	26.890023773883946	24.58835960200757
50-51	22.982301664171874	24.972263802060404	27.452672360658624	24.592762173109094
52-53	22.82997270405917	24.84811129699745	29.108039094831383	23.213876904112002
54-55	22.168706524610375	25.03389979748173	28.28211675618561	24.515276921722286
56-57	22.62921546182971	25.05503213876904	27.145372897772297	25.170379501628954
58-59	21.930967685128113	24.804085585982214	28.715329752575503	24.549616976314166
60-61	22.65210883155763	25.46535176543101	27.458835960200755	24.423703442810602
62-63	20.986175926741215	26.904112001408826	27.177071409703267	24.932640662146692
64-65	21.73197147133926	26.64788236330017	26.537818085762083	25.082328079598486
66-67	21.558510169939243	26.7297701857885	26.19529805406357	25.516421590208683
68-69	21.491591089196092	26.890023773883946	26.080831205423966	25.537553931495992
70-71	22.025182706700715	26.573919168794575	25.753279915470635	25.64761820903407
72-73	21.95650259751695	25.687241348947786	25.728625517302106	26.62763053623316
74-75	21.706436558950426	26.115171260015853	25.59038478471427	26.58800739631945
76	22.306947257198203	25.769129171436116	25.342960288808662	26.580963282557015
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	3.5
15	8.0
16	16.5
17	24.0
18	25.5
19	36.0
20	62.5
21	80.0
22	85.5
23	113.0
24	190.5
25	246.0
26	278.5
27	317.5
28	385.0
29	446.0
30	518.5
31	692.0
32	909.5
33	1026.0
34	1135.5
35	1325.5
36	1612.0
37	1818.0
38	1988.0
39	2351.5
40	2713.5
41	3026.5
42	3171.0
43	3277.0
44	3489.0
45	3572.5
46	3550.0
47	3305.5
48	3028.0
49	2768.5
50	2542.0
51	2363.0
52	1991.0
53	1694.5
54	1591.0
55	1481.5
56	1303.5
57	1158.5
58	1082.0
59	917.0
60	733.0
61	665.5
62	617.0
63	615.0
64	598.5
65	600.0
66	606.5
67	597.0
68	585.5
69	585.0
70	548.5
71	501.0
72	513.5
73	487.5
74	434.0
75	419.0
76	395.0
77	349.0
78	286.0
79	245.0
80	209.5
81	154.5
82	106.5
83	78.0
84	72.5
85	50.5
86	26.5
87	19.0
88	11.0
89	3.0
90	2.5
91	1.0
92	0.0
93	0.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	56785.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.08884388482874
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.79483600837405	46.55630888438848
2	15.296580600139567	19.300871709078102
3	4.926727145847871	9.324645593026327
4	2.322400558269365	5.860702650347803
5	1.1500348918353105	3.6277185876551905
6	0.8234473133286812	3.117020339878489
7	0.43265875785066293	1.9107158580611077
8	0.3098394975575715	1.5637932552610725
9	0.2288904396371249	1.2996389891696751
>10	0.7062107466852757	5.980452584309236
>50	0.005582693649685973	0.2201285550761645
>100	0.0	0.0
>500	0.0027913468248429866	1.238002993748349
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGT	703	1.238002993748349	No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCG	64	0.1127058201989962	No Hit
CTTATACACATCTCCGAGCCCACGAGACAGACCAGCACCTCGTATGCCGT	61	0.10742273487716826	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.001761028440609316	0.0
20	0.0	0.0	0.0	0.001761028440609316	0.0
21	0.0	0.0	0.0	0.001761028440609316	0.0
22	0.0	0.0	0.0	0.001761028440609316	0.0
23	0.0	0.0	0.0	0.003522056881218632	0.0
24	0.0	0.0	0.0	0.003522056881218632	0.0
25	0.0	0.0	0.0	0.003522056881218632	0.0
26	0.0	0.0	0.0	0.003522056881218632	0.0
27	0.0	0.0	0.0	0.010566170643655896	0.0
28	0.0	0.0	0.0	0.02113234128731179	0.0
29	0.0	0.0	0.0	0.02993748349035837	0.0
30	0.0	0.0	0.0	0.03698159725279563	0.0
31	0.0	0.0	0.0	0.05635291009949811	0.0
32	0.0	0.0	0.0	0.0774852513868099	0.0
33	0.0	0.0	0.0	0.10213964955534031	0.0
34	0.0	0.0	0.0	0.14264330368935457	0.0
35	0.0	0.0	0.0	0.22012855507616447	0.0
36	0.0	0.0	0.0	0.30641894866602093	0.0
37	0.0	0.0	0.0	0.43673505327111034	0.0
38	0.0	0.0	0.0	0.6286871532975258	0.0
39	0.0	0.0	0.0	0.8118341111208947	0.0
40	0.0	0.0	0.0	1.0953596900589944	0.0
41	0.0	0.0	0.0	1.4229109800123272	0.0
42	0.0	0.0	0.0	1.762789469049925	0.0
43	0.0	0.0	0.0	2.151976754424584	0.0
44	0.0	0.0	0.0	2.7031786563352997	0.0
45	0.0	0.0	0.0	3.300167297701858	0.0
46	0.0	0.0	0.0	3.858413313375011	0.0
47	0.0	0.0	0.0	4.485339438231928	0.0
48	0.0	0.0	0.0	5.098177335563969	0.0
49	0.0	0.0	0.0	5.644096152152858	0.0
50	0.0	0.0	0.0	6.209386281588448	0.0
51	0.0	0.0	0.0	6.7781984679052565	0.0
52	0.0	0.0	0.0	7.364620938628159	0.0
53	0.0	0.0	0.0	7.907017698335828	0.0
54	0.0	0.0	0.0	8.54627102227701	0.0
55	0.0	0.0	0.0	9.159108919609052	0.0
56	0.0	0.0	0.0	9.708549793079158	0.0
57	0.0	0.0	0.0	10.331953861054856	0.0
58	0.0	0.0	0.0	10.96768512811482	0.0
59	0.0	0.0	0.0	11.522409086906753	0.0
60	0.0	0.0	0.0	12.107070529189047	0.0
61	0.0	0.0	0.0	12.637140089812451	0.0
62	0.0	0.0	0.0	13.25702210090693	0.0
63	0.0	0.0	0.0	13.794135775292771	0.0
64	0.0	0.0	0.0	14.331249449678612	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGTAT	15	0.0022139922	70.0	39
ATATCGT	15	0.0022139922	70.0	37
TCTATAC	30	1.0068288E-7	70.0	3
GCATATC	15	0.0022139922	70.0	35
CAGCATA	25	2.3716534E-4	56.000004	33
TCCATAC	20	0.0069182026	52.5	2
ACAAAGT	20	0.0069182026	52.5	2
CATATCG	20	0.0069182026	52.5	36
CGTCTTC	140	0.0	52.5	48
CTCTTGC	20	0.0069182026	52.5	1
CATTCCC	20	0.0069182026	52.5	25
CCGTCTT	140	0.0	52.5	47
ACCTAAG	20	0.0069182026	52.5	38
CGCTCAT	20	0.0069182026	52.5	65
CTAAACA	20	0.0069182026	52.5	4
ATAACAT	20	0.0069182026	52.5	36
GTAAGAC	20	0.0069182026	52.5	3
TATCGTA	20	0.0069182026	52.5	38
GCCGTCT	140	0.0	52.5	46
CGTATGC	155	0.0	51.935482	41
>>END_MODULE