FastQCFastQC Report
Wed 25 May 2016
SRR1781144_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781144_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61030
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGCCG150.002214791370.064
GCTTGTA150.002214791370.01
CCAGTAA150.002214791370.026
CGTTACT252.3730705E-456.00000417
CGACGTT200.006920677652.50000414
CCGATGC200.006920677652.50000433
ACGTTAC200.006920677652.50000416
GACGTTA200.006920677652.50000415
GACCGGG200.006920677652.50000432
CCGGAAT200.006920677652.5000043
AAGGTGA200.006920677652.50000453
TTCCGGC200.006920677652.50000454
TCGGCCA200.006920677652.50000421
ATCCGGA200.006920677652.5000041
CAAGGGT200.006920677652.50000432
CGCTACA200.006920677652.50000424
GGTATAC200.006920677652.50000439
ACTCGGA200.006920677652.50000421
TACTCGG200.006920677652.50000420
TACACGA200.006920677652.50000427
Produced by FastQC (version 0.11.5)